1. Fill a 250-ml Erlenmeyer flask with approximately 100ml of water and
position it at the approximate geometric center of the shaking
platform.
2. Install a thermocouple inside the flask with the thermocouple junction
in direct contact with the water.
3. Press and hold the “star” (*) key and using the up or down arrow key,
adjust the set point to the desired temperature.
4. Allow the shaker to run until chamber temperature has stabilized.
5. The controller display should now be indicating the set point
temperature. Make note of the thermometer reading.
6. Press and hold both arrow keys until the controller display indicates
“tunE”. Release the arrow keys. Press and release the down arrow key,
the display should now alternate between “LEUL” and “1”. Press and
hold the “star” (*) key and using the up arrow key adjust the display to
read “3”. Release the “star” (*) key. The display should now alternate
between “LEUL” and “3”. Press and release the up arrow key until the
display indicates “Zero”. The display should now alternate between
“Zero” and a numerical value.
7. Using the examples shown in the table
at right and the thermometer value
obtained in Step 5 above, enter the
correct “Zero” value into the controller
by pressing the “star” (*) key and using
the up or down arrow key. If there is
already a “Zero” value present, then add
the new value to the one already
present.
8. When the correct “Zero” value has been entered, press and hold the
two arrow keys together until the display again indicates the chamber
temperature. If the procedure was done correctly, the controller display
should now agree with the thermometer reading to within ±0.5°C.
9. Allow the unit to run for at least an additional 30 minutes.
10. Re-check the thermometer reading. The controller display and the
thermometer should agree to within ±0.5°C. If not, repeat Steps 6, 7
and 8.
Incubated and Refrigerated Shakers 5-3
Thermo Scientific
Section 5
Operation
Temperature
Calibration
Thermometer
=
60°C
Controller Reading
=
65°C
Subtract
=
-5°C
Enter Zero value of -5°C
Thermometer
=
70°C
Controller Reading
=
65°C
Subtract
=
+5°C
Enter Zero value of +5°C
