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Thermo Scientific KingFisher Pure RNA Blood Kit
Thermo Fisher Scientific
Appendix A
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Troubleshooting
Problem
Possible cause and actions
Magnetic particles
remaining in the
sample or elution well
Starting material that is too viscose prevents efficient collection of
the KingFisher Magnetic Beads from the lysed sample.
The magnetic rods will not be able to collect all the particles
unless the viscose samples are diluted before the beginning of
the purification process. Improper lysis may also cause problems
collecting the KingFisher Magnetic Beads.
If the KingFisher Magnetic Beads are inefficiently collected from
the Elution Buffer, the addition of a small amount of detergent
(e.g. Tween™ 20) may improve the results.
KingFisher Magnetic Beads that occasionally remain attached to
the tip combs at the end of the process do not affect the total RNA
yield, as the RNA has already been released from the KingFisher
Magnetic Beads into the Elution Buffer.
If the KingFisher magnetic particle processor does not work
properly, refer to the relevant user manual of the KingFisher
instrument in use.
Cont.
