Thermo Scientific KingFisher Pure RNA Blood Kit
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Thermo Fisher Scientific
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Storage Conditions and
Preparation of the Reagents
Storage Conditions
Upon arrival of the kit, store the DNase I, DNase I Reconstitution Buffer,
and 2 x DNase Buffer at -20°C. The Proteinase K solution is stable at room
temperature until the seal of the vial is broken. After being opened, the vial
should be stored at -20°C. Store the KingFisher Magnetic Beads at +4°C.
Other kit components can be stored at room temperature (15−25°C). The
reagents are stable for up to two years from the manufacturing date.
Preparation of the Lysis Buffer
Before each RNA purification experiment, a fresh aliquot of Lysis Buffer
should be supplemented with 2 M DTT solution (not provided). It is required
to use 200 µl of Lysis Buffer for the purification of one sample. Add 20 µl of
2 M DTT to 1 ml of Lysis Buffer.
Preparation of the Wash Buffers and
Rebinding Buffer
Add 100% isopropanol and 96−100% ethanol to the Wash Buffer 1, Wash
Buffer 2, and Rebinding Buffer, as indicated below in
prior to the
first use.
