Thermo Scientific KingFisher Pure RNA Blood Kit
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19
Thermo Fisher Scientific
Protocols and Pipetting Instructions
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Chapter 5
3. Place a Thermo Scientific™ KingFisher™ Flex 96 tip comb for deep
well magnets on a
Tip Plate
(i.e. an empty KingFisher Flex 96 KF plate).
4. Start the PURE_RNABlood_Flex96 protocol using the KingFisher Flex 96
and load the plates as instructed on the KingFisher Flex 96 instrument
display.
Switch on the KingFisher Flex making sure that you are using the Thermo
Scientific™ KingFisher™ Flex 96 deep well head and heating block.
Connect the PC with BindIt Software 3.2 to the KingFisher Flex. Start the
PURE_RNABlood_Flex96 protocol. Insert the Tip Plate and the filled plates
into the instrument as indicated on the KingFisher Flex display. After all the
plates have been loaded into the instrument, the protocol will start.
5. When the KingFisher Flex is to be run as a standalone instrument,
transfer the PURE_RNABlood_Flex96 protocol to the KingFisher Flex.
The instructions for transferring the protocol can be found in Chapter 4:
“Using the software” in the
BindIt Software for KingFisher Instruments
version 3.2 User Manual.
6. Add the KingFisher Magnetic Beads and isopropanol to the Sample plate
during the first dispense step.
When the KingFisher Flex pauses at the dispense step after the lysis step at
approximately 5 minutes after starting the protocol run, remove the Sample
plate from the instrument, and add the KingFisher Magnetic Beads and
isopropanol to the
Sample plate
.
Plate name
Add
Added reagent volume
per well
Sample
Isopropanol
KingFisher Magnetic Beads*
400 µl
15 µl
* Resuspend the KingFisher Magnetic Beads well by vortexing before use.
7. Place the Sample plate back into the instrument and press
Start
.
8. Add the Rebinding Buffer (supplemented with isopropanol) to the
Sample plate during the second dispense step.
