Thermo Scientific HM525 NX Operator Guide
54
Heat Extractor
1 Cryobar
2 Cryo-Mold
3 Specimen Chuck
4 Heat Extractor
5 Cryo-Mold handle
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Using a Heat Extractor in combination with a cryo mold or specimen stage promotes a faster
freezing method which freezes from the top and the bottom simultaneously.
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It also reduces the formation of freezing artifacts.
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The specimen stage is inserted into the fast freezing rail and the specimen mounted. A cryo mold
can also be used.
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The Heat Extractor is then placed horizontally on top of the specimen.
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When the specimen has completely frozen remove the Heat Extractor.