Thermo Scientific HM525 NX Series Operator'S Manual Download Page 1

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Thermo Scientific HM525 NX

Product Code 956640, 956641,

956650, 956651

Operator Guide

388159 Issue 10

Summary of Contents for HM525 NX Series

Page 1: ...Thermo Scientific HM525 NX Product Code 956640 956641 956650 956651 Operator Guide 388159 Issue 10 ...

Page 2: ...Thermo Scientific HM525 NX Operator Guide 2 ...

Page 3: ... up to date and relates to the condition of the product If necessary check with your local Thermo Fisher Scientific representative This document may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or other form without prior written consent of Thermo Fisher Scientific All information contained in this manual is proprietary and confidential and the...

Page 4: ...ases Refrigerant R404A R404A GWP value 3992 System charge weight 0 182 Kg System CO2 equivalent charge weight 0 713 tonnes HM525 NX 230V EC Regulation No 517 2014 Fluorinated Greenhouse Gas Regulations Contains fluorinated greenhouse gases Refrigerant R404A R404A GWP value 3992 System charge weight 0 190 Kg System CO2 equivalent charge weight 0 758 tonnes ...

Page 5: ...1326 2 6 2012 and IEC 61326 1 2012 This equipment has been designed and tested to CISPR 11 2009 A1 2010 Class A It is intended for use in a laboratory environment by a trained and qualified professional In a domestic environment it may cause radio interference in which case it may be necessary to take measures to mitigate the interference ...

Page 6: ...are available on this instrument and the instructions contained in this manual are not specific to any one variant Thus some features described may not be available on your instrument This IFU is valid for the variants listed below Product Code Product Variant 956640 HM525 NX 100 120V 50 60 Hz 965641 HM525 NX UV 100 120V 50 60 Hz 956650 HM525 NX 220 230V 50 60Hz 956651 HM525 NX UV 220 230V 50 60Hz...

Page 7: ...nual and on the instrument to indicate that hand injuries are possible due to moving parts Take suitable precautions Cutting hazard sharp edges watch your fingers This symbol is used on the instrument or in a document to indicate that irritants or potentially harmful chemicals are present Refer to the Material Safety Data Sheets for the products and always use Good Laboratory Practice This symbol ...

Page 8: ... Guide 8 Serial number It is stated on the product label sticker It is built up as follows SYYMMXXXX S Production site Y year of production M Month of production X Counter So the serial number states the production date of the device SN ...

Page 9: ...6 Microtome and Sectioning Specifications 16 Factory Settings 17 CHAPTER 2 INSTRUMENT SETUP 18 UNPACKING AND REPACKING 18 Safety Precautions 18 UNPACKING PROCEDURE 18 General Information 18 Unpacking the Instrument 19 Repacking the Instrument 21 LOCATION AND SETUP 22 IDENTIFICATION OF PARTS 23 Overview 23 Connections Panel 23 Cryochamber 23 SOLID KNIFE HOLDER 24 Inserting the Knife 24 Height Adjus...

Page 10: ...FINE SECTION THICKNESS 38 Set the Fine Section Thickness 38 MULTI FUNCTION BAR 39 Trim Button 39 Trimming 39 Time and Date 39 Outstanding Service Message 40 Counter 40 Retraction 41 HANDWHEEL LOCK 42 MENU 42 LANGUAGE 42 Set the Language 42 TIME AND DATE 43 Set the Date 43 Set the Day 43 Set the Month 43 Set the Year 43 Change to ISO Format 44 Setting the Time 44 Set the Minutes 44 Set the Hour 44 ...

Page 11: ...nical Handwheel Brake 57 HOW TO AVOID MALFUNCTIONS 58 CHAPTER 4 TROUBLESHOOTING 59 TROUBLESHOOTING TABLES 59 TIPS FOR SUCCESSFUL SECTIONING 62 TEMPERATURE LIST FOR CRYO SECTIONING 63 CHAPTER 5 MAINTENANCE AND CARE OF THE HM525 NX 64 CLEANING THE TOUCH SCREEN DISPLAY 64 SHUTTING OFF FOR CLEANING 64 CLEANING AND CARE 65 Cleaning and Care during Routine Work 65 Cleaning the Cooling Vent 65 CONDITIONS...

Page 12: ...oyed and consideration must be given to the potential for hazard when dealing with these chemicals Do not use the instrument in close proximity to strong electromagnetic radiation as these may interfere with the proper operation The electromagnetic environment should be evaluated prior to operation of the device Good Laboratory Practice must be used when handling tissue samples to prevent cross co...

Page 13: ...ive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific on behalf of Thermo Shandon Limited has contracts with one or more recycling disposal companies in each EU Member State and this product and packaging should be disposed of or recycled through them For further information contact your Thermo Fisher Scientific service representative Warranty Statement Thermo Shandon Limi...

Page 14: ...ent may only be operated within the scope of its intended use as described above and as per the instructions given in this manual Any other use of this instrument is considered as improper operation Instrument Features The HM525 NX is a precision engineered ergonomically designed high performance cryostat in modular design with a stainless steel cooling chamber Some of its features are Colour touc...

Page 15: ...Indoor Use Only Temperature Operating Limits 5 C to 35 C 41 F to 95 F at a max Relative humidity of 60 Temperature Recommended Operation 15 C to 30 C 59 F to 86 F Note Performance may deteriorate when operated outside of this range Temperature Transport and Storage 20 C to 50 C 4 F to 122 F Relative Humidity Max 60 RH up to 35 C Altitude Up to 2000 m 6 500 ft Pollution Degree 2 Over Voltage Catego...

Page 16: ... 50 µm in 5 µm steps 50 µm 100 µm in 10 µm step Trimming Thickness Range 5 500 µm Trimming Thickness Resolution 5 30 µm in 5 µm steps 30 µm 100 µm in 10 µm steps 100 µm 200 µm in 20 µm steps 200 µm 500 µm in 50 µm steps Specimen Retraction Return Travel 20 µm Vertical Specimen Stroke 64 mm Horizontal Specimen Movement 28 mm Specimen Approach Automated via arrow buttons Chuck Size 30 40 mm addition...

Page 17: ...ettings Counter Mode C 0 Retraction ON Language English Chamber Temperature 20 C Fine 1 µm Trim 5 µm Chamber Light 100 Screen Brightness 100 Stepper Motor Initialization Yes Cryobar OFF Defrost Cycle Automatic ON 00 00 Night Section Counter 0 Section Sum 0 ...

Page 18: ...e damage to the unit Note The packing materials should be carefully stored for use in any future instrument shipments Unpacking Procedure General Information Inspect the packaging if it is damaged and or the contents do not match the supplied packing list inform your local Thermo Fisher scientific representative Ensure that these Instructions are retained for future use The HM525 NX is delivered o...

Page 19: ...ng the handle fig 1a item 2 The hinges of the upper wooden cover fig 1 item 1 are inserted into the screws of the bottom plate fig 1a item 3 The correct side for this is marked with arrows The tool fig 2a item 2 for the height adjustment of the setting feet of the HM525 NX is packed together with the accessories of the HM525 NX separate carton on the foot rest of the HM525 NX Turn the setting feet...

Page 20: ...e between the wall and rear panel is approx 15 cm For this install the distance bolts fig 2 item 1 The suction vent areas on either side are kept free fig 2 item 2 The mains switch for separating the instrument from the power supply is accessible at any time Fig 2 The installation site must also be free from Draught by open doors or by air conditioning systems Direct exposure to sunlight into the ...

Page 21: ...h the housing of the HM525 NX It is absolutely necessary to remove this transportation screw before the initial turn on Loosen the transportation screw fig 2b item 1 via the Allan key size 6 Now fix the separately packed handwheel handle on the handwheel with the attached screw by means of the Allan key Fig 2b Repacking the Instrument To repack the instrument follow the above instructions in rever...

Page 22: ...es The mains power socket must be accessible at all times The location must be free from draughts open doors or air conditioning systems The location must not expose the cryo chamber to direct sunlight Note These measures are intended to reduce the build up of frost and maintain consistent cooling temperatures resulting in more favourable working conditions Locations which have high humidity and o...

Page 23: ...hamber Window 3 Touch Screen 4 Connections Panel 5 Handwheel Mechanical Lock 6 Handwheel 7 Water Drain Reservoir Connections Panel 8 USB port 9 Power Switch 10 Power Socket Cryochamber 11 Cryobar and Cooled Storage Area 12 Specimen Head 13 Tool storage 14 Handwheel Mechanical Brake Button 15 Waste Tray ...

Page 24: ...unter clockwise direction to put the anti roll plate against the knife Note Insert the glass anti roll plate parallel towards the blade edge Then tighten the clamping screws to fix the knife into position If the cutting area of the knife is no longer usable the knife can be moved Open the clamping screws and move the knife to the left or right side as required Fix the knife again via the clamping ...

Page 25: ... knurled screw Selecting the clearance angle Loosen the clamping lever on the left side of the knife carrier Swivel the upper part of the knife carrier on the base until the desired clearance angle is reached The clearance angle can be read on the scale at the left side of the base Note Usable cuts are only achieved at a clearance angle of 10 or more ...

Page 26: ...ile blades first loosen the lever and remove the spacer strip For this push in the stop bolt and turn the clamping lever in a counter clockwise direction over the stop bolt Remove the clamping lever together with the eccentric bolt First remove the clamping plate and then the spacer strip if necessary Fix the clamping plate with the clamping lever again in the blade carrier in reverse order To ins...

Page 27: ...g adjustment of specimen always position the knife guards over the blade edge Fine adjustment of the anti roll device The fine adjustment of the anti roll device is carried out via the knurled screw Selecting the clearance angle Loosen the clamping lever Swivel the upper part of the blade carrier on the base until the desired clearance angle is reached The clearance angle can be read on the scale ...

Page 28: ...if necessary Fix the clamping plate with the clamping lever again in the blade carrier in reverse order To insert the blade now loosen the clamping lever Press the clamping plate on its lower area to open the slot behind the clamping plate Insert the blade from the left side into the slot behind the clamping plate as far as possible Tighten the clamping lever fixing the blade in its position To se...

Page 29: ...the base until the desired clearance angle is reached The clearance angle can be read on the scale on the left side of the base Bring the clamping lever into clamping position The selected clearance angle is now fixed in its position Note Usable cuts are only achieved at a clearance angle of 10 or more Specimen Head HM525 NX 1 Microtome base plate 2 Electrical connector 3 Specimen Chuck Release Le...

Page 30: ...es should be stored on the shelves or in the brush tray in the cryo chamber The exceptions to this rule are the specimen chucks which should be kept at room temperature The heated sliding window should be closed during periods of inactivity and while preparing specimens to avoid warm air getting into the chamber This will result in more favourable working conditions and a lower frost build up The ...

Page 31: ...the chuck Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge Use the specimen chuck release lever to orient the specimen on the x and y axes Note The specimen chuck release lever will click into place when the centre position has been reached Tighten the specimen head clamping lever again For further specimen orientation the specimen chuck can ...

Page 32: ...X as the compressor needs high surge currents when started Do not use multi socket power outlets with small wire sizes for the supply of the instrument Insert the supplied power cord into the power socket and then connect the power cord to the mains supply Install the blade holder if necessary Close the heated window Turn on the power switch Note The default setting of the specimen head temperatur...

Page 33: ...switched on 1 Fast forward movement of specimen head 2 Slow forward movement of specimen head 3 Fast Backward movement of specimen head 4 Trim thickness 5 Chamber temperature 6 Section thickness 7 Remaining Travel 8 Cryobar button 9 Menu button 10 Position button 11 Retraction button 12 Counter mode sum of sections number of sections 13 Time Date button 14 TRIM button Press the required button to ...

Page 34: ...djust the value to the required setting 1 Decreases the selected value by multiple steps 2 Decreases the selected value by 1 step 3 Increases the selected value by 1 step 4 Increases the selected value by multiple steps Note The number of steps by which the multiple step increase decrease buttons alters the selected value will vary depending on the function ...

Page 35: ...icating by the timeline display below the cryobar button on the screen The specimen is frozen onto the specimen chuck using a freezing compound and the cryobar Note To ensure optimal adhesion of the specimen to the chuck store the chucks at room temperature prior to use Press the Cryobar button on the Main screen to activate it The Cryobar button is highlighted in blue when the function is activat...

Page 36: ... 3 seconds The new position will be stored To reset the position hold down the position button for approx 6 seconds Backward fast movement of specimen head 1mm sec button Press the button for 1 sec to move the Specimen Head backwards a short distance After about 2 sec the head moves back automatically Press the button again to stop the movement immediately Slow forward movement of specimen head 0 ...

Page 37: ...me Chamber Temperature button on the Main screen Either select one of the preset temperatures 35 C 30 C 20 C or 10 C or adjust the temperature using the arrow keys Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Note The new set temperature is displayed in white on the Main screen until the temperatur...

Page 38: ... to the Main screen Press the Back button to exit back to the Main screen without saving the changes Set the Fine Section Thickness Press the Fine Section Thickness button on the Main screen The Fine Section screen will be displayed Either select one of the preset values 100µm 50µm 10µm or 5µm or adjust the value using the arrow buttons For details of the thickness steps see Technical Specificatio...

Page 39: ... set trimming can be carried out using the trimming function Trimming Make sure that the trimming thickness setting is selected Note Trimming thickness is shown highlighted on the display Turn the hand wheel in a clockwise direction to begin trimming Press the Trim button to move forward the blade holder by the preset trim value The hand wheel does not need to be turned to utilize this function No...

Page 40: ... The section counter adds up the number of the produced sections After each downward movement of the specimen head the number on the section counter increases by 1 To reset the section counter Press the Section Counter button for approximately 3 seconds to reset it to zero Section Thickness Sum The Section Thickness Sum adds up the total number of microns that have been cut To reset the section co...

Page 41: ...the OK button to turn off the Retraction and return to the Main screen The button will change to white and display R Off Press the Back button to return to the Main screen without saving the changes Turn on the Retraction Enter the Retraction menu screen as described above Press the OK button on the screen to turn on the Retraction The button will display R On Press the Back button to return to th...

Page 42: ...ton The Menu screen will be displayed Press the Back button to return to the Main screen Press the desired function button to access the respective submenu Language Set the Language Press the Menu button on the Main screen The Menu screen will be displayed Press the Language button The Menu Language screen will be displayed Select the desired language by pressing the respective button Press OK to ...

Page 43: ...ay button to highlight it Use the arrow buttons to select the desired day and date Set the Month Press the Month button to highlight it Use the arrow buttons to select the desired month Set the Year Press the Year button to highlight it Press the Year button to highlight it Press OK to confirm the setting and to return to the Main screen Press the Back button three times to exit back to the Main s...

Page 44: ...e displayed Press the Time button The Time screen will be displayed Set the Minutes Press the Minute button to highlight it Use the arrow keys to select the desired minute Set the Hour Press the Hour button to highlight it Use the arrow keys to select the desired hour Set the Time Format Press the 12 24 button to toggle between the 12 and 24 hour modes Note The 12 24 button is highlighted in yello...

Page 45: ...UV screen will be displayed Select Duration Now or Time Set the Duration of the UV Cycle Press Duration to choose between 4h 3h 2h or 1h or use the arrow buttons to select the duration of the UV cycle To select the Now option Press Now button to start and immediate UV cycle Press abort to stop the cycle Press OK when the Errorcode 007 003 window comes up ...

Page 46: ...e the arrow keys to set the additional minutes to the hour for the UV cycle to start on the indicated day Press OK to confirm the settings and to return to the Main screen Press the Back button three times to exit back to the Main screen without saving the changes Do not open the window during the disinfectant cycle Do not look directly into the UV light Note The average life span of a UVC lamp is...

Page 47: ...ck button twice to exit back to the Main screen without saving the changes Note The chamber illumination can be adjusted from the lowest 0 to the highest 100 level Standby Mode When the HM525 NX is switched on but has been inactive for 1 hour it will automatically enter the Standby mode The HM525 NX maintains the user adjusted temperature during Standby In Standby mode the chamber light switches o...

Page 48: ...he efficiency of the evaporator For this reason the daily defrosting is necessary Remove the specimens from the cryo chamber during defrosting as the temperature inside the chamber will increase Do not leave or store specimens inside the HM525 NX over a long period of time Due to a power failure or other unexpected malfunctions of the instrument specimens might be damaged Note Set the time of the ...

Page 49: ... return to the Main screen without starting the defrosting Press the Now button to start the defrosting immediately The Defrosting screen will appear showing the progress of the defrosting and the actual temperature of the evaporator Abort the Defrost Press the Abort button on the Defrosting progress screen The Main screen will appear displaying an error message Press the OK button to remove the e...

Page 50: ... Main screen The Menu screen will be displayed Press the Defrost button The Defrost screen will be displayed Press the Cyclic button The Defrost Time screen will be displayed Note The standard setting for the daily defrost cycle is daily at 00 00 a m To change the standard setting press the Hour and or the Minute button Adjust the time by using the arrow keys Press the OK button to confirm your se...

Page 51: ...een will be displayed Password has to be provided by service technician After entering the right password Service only Log File screen will be displayed Use the scroll bar on the right side to scroll through the Log File Insert the USB Stick Note The Export button is now highlighted in white The Export button is now highlighted in white Press the Export button to transfer the log data to the origi...

Page 52: ...the Back button three times to return to the Main screen without restoring the factory settings Note A message appears on the Main screen to inform the user that the factory settings have been restored The message disappears after approximately 3 seconds Display Icons with Text The text underneath the Main screen icons can be switched off if required To switch the text off Enter the factory settin...

Page 53: ...lect one of the preset values 25 50 75 or 100 or adjust the brightness using the arrow keys Press the OK button to restore the factory settings and to return the Main screen Press the Back button three times to return to the Main screen without saving the changes Note The chamber illumination can be adjusted from the lowest 0 to the highest 100 levels Service This submenu is for service purposes o...

Page 54: ...en stage promotes a faster freezing method which freezes from the top and the bottom simultaneously It also reduces the formation of freezing artifacts The specimen stage is inserted into the fast freezing rail and the specimen mounted A cryo mold can also be used The Heat Extractor is then placed horizontally on top of the specimen When the specimen has completely frozen remove the Heat Extractor...

Page 55: ...not be changed Trimming and First Cuts After the specimen orientation and the blade angle have been set trimming can be carried out using the trimming function Trimming Make sure that the trimming thickness setting is selected Note Trimming thickness is shown highlighted on the display Turn the hand wheel in a clockwise direction to begin trimming Press the Trim button to move forward the blade ho...

Page 56: ... hand wheel in a clockwise direction to carry out sectioning Note The section slides into the space between the blade and the anti roll plate Use the handle to move the anti roll plate assembly away from the specimen The section should remain on the clamping plate Arrange specimen section gently with a brush Transfer the specimen onto a glass microscope slide with help of adhesion forces ...

Page 57: ...he mechanical handwheel lock acts as an additional safety device especially when the instrument is turned off To mechanical handwheel lock can be activated by the lever on the handwheel To activate the mechanical Handwheel Lock Bring the handwheel handle into a 12 o clock position The switch for the handwheel lock can now be pressed forward into the locked position Note If the lever is not in the ...

Page 58: ...temperature according to the specimen Select the appropriate freezing medium Note If the specimen was frozen with liquid nitrogen or similar freezing techniques the specimen must adapt itself to the cutting temperature Carefully select the required knife material and profile Adjustment of knife height Adjustment of proper clearance angle Usable cuts are only achieved at a clearance angle of 8 12 o...

Page 59: ...otome Draught open doors windows Change site of installation close windows doors Retarded freezing on of the specimen Surface of the fast freezing station is soiled Remove debris Lower part of the chuck is soiled or damaged Remove debris remove damage Static electricity in chamber Chatter while cutting Unfavourable clearance angle Readjust clearance angle Sections tear or crack Insufficient knife ...

Page 60: ...en selected and the anti roll plate has been adjusted correctly Knife and or anti roll plate are not clean Clean with a dry cloth or brush Edge of anti roll plate is damaged Replace anti roll plate Blunt knife Move replace sharpen the knife Sections break fissures in the sections sections are not stretched properly Specimen too cold Select a higher temperature Static charge draught Remove the caus...

Page 61: ...uch and scratches on the specimen Readjust the anti roll plate Thick thin sections Knife sharpness Move the knife if necessary replace or sharpen Knife angle Readjust Clamping on knife carrier Check clamping Clamping on specimen holder Check clamping Tight handwheel punctual during the entire movement Debris and section waste between microtome and base plate Remove and clean Soiled link block Toot...

Page 62: ...ate chamber temperature according to the specimen type see Temperature list for Cryo sectioning Allow time for the temperatures within the chamber to stabilise Select the appropriate freezing compound Neg50 Avoid excessive use of freezing compound Note If the specimen was frozen with liquid nitrogen or similar freezing techniques the specimen must be allowed to adjust to the cutting temperature Ad...

Page 63: ...C Liver Kidney Spleen Thyroid Lymph Node Uterine Curettings Tongue Testicle Range B 20 to 30 C Muscle Breast without fat Brain Bone Marrow Lungs Intestine Prostate Cervix Uterus Pancreas Adrenal Skin without fat Range C 30 to 60 C Adipose Omentum Breast with fat Cheese Butter Industrial Waxes Soft Rubber Additional cooling with liquid nitrogen or dry ice might be necessary Note The indicated cutti...

Page 64: ...e danger of freezing burns from contact with frozen parts and potentially contaminated material it should only be performed by skilled or trained personnel Please proceed as follows Wear protective gloves Remove knife blade from the knife carrier and store it in a knife case Remove the brush shelf tools as well as chucks Bring the handwheel handle into its upper position i e the specimen clamping ...

Page 65: ...lly contaminated parts of the instrument is highly recommended All commercially available HM525 NX disinfection solutions can be used Cleaning the Cooling Vent Open the cleaning door with a coin on the left and right side Remove the dust from the cooling grill vent by means of a commercially available vacuum cleaner Note Carry out this cleaning in regular intervals thus extending the lifetime of t...

Page 66: ...losing down Turn off the instrument Unplug the unit Remove blade and or knife from blade and or knife carrier Remove section waste trays brush shelf blade knife carrier and other accessories as well as tools from the cryo chamber Note Clean and disinfect these accessories according to the lab regulations and transport them in dry condition Clean and disinfect the cryo chamber according to lab regu...

Page 67: ... Insert the wooden cover Attach the tightening strips around the packing Disposal of the Instrument after Final Shutdown After the final shutdown of the instrument we recommend to contact a local recycling company for disposal according to the national applicable regulations To be applied in countries of the European Union and other European countries with a separate collecting system within the w...

Page 68: ...key 6 mm 362120 1 flat brush 334170 1 brush 334169 Open end Wrench 362741 1 brush shelf 415640 Waste Tray Long 415620 Waste Tray Small 415600 Deposit Rack right side 432770 Deposit Rack left side 432780 Heat Extractor 524510 Additional equipment optional Description Cat No Specimen chucks Ø 20 mm 715700 Ø 30 mm 715220 Ø 40 mm 715230 50 x 50 mm 715730 55 x 55 mm 715740 60 x 55 mm 715750 70 x 55 mm ...

Page 69: ...ade carrier EC 70 705470 Disposable Blade Holder EC 449980 Anti Roll Plate ARP for use with EC70 449990 Anti Roll Plate ARP for use with EC Other accessories 524490 Heat Extractor HM525NX Operator Guide 705460 English 388159 German 388160 Italian 388161 French 388162 Russian 388163 Dutch 388164 Chinese 388165 Spanish 388166 Japanese 388167 ...

Page 70: ...e Adjustment of the Anti Roll Device 29 Inserting the Blade 28 Selecting the Clearance Angle 29 Disposal of the Instrument after Final Shutdown 67 E Electrical Specifications 15 EMC Statement 5 Enter the Log File 51 Environmental Specifications 15 F Factory Settings 17 52 Fine adjustment of the anti roll device 25 G General Information 18 H Hand Wheel Lock 57 Handwheel Lock 42 Heat Extractor 54 Ho...

Page 71: ... Section Thickness 38 Set the Language 42 Set the Month 43 Set the Specimen Head Position 36 Set the Year 43 Setting Preferences 35 Setting the Hour 44 Setting the Minutes 44 Setting the Temperature 37 Setting the Time 44 Setting the Time Format 44 Setup 30 Shutting Off for Cleaning 64 Solid Knife Holder 24 Height Adjustment of the Knife 24 Inserting the Knife 24 Specimen Head HM525 NX 29 Specimen...

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