6
Running an Xcalibur Experiment by Using ETD
Data Dependent NL MS
3
Experiment
50
MS/ETD System Getting Started Guide
Thermo Scientific
12. In the Isolation Width (
m/z
) box, enter a value between
2
and
3
.
The Normalized Collision Energy and Activation Q parameters are unavailable because
they are not applicable to ETD.
13. In the Activation Time (ms) box, leave the default value or enter a different value as
described in
“Optimizing the Reagent Ion Reaction Time”
.
14. In the Tune Plus window, click the
Save
(
) button.
15. Browse to the
drive
:\Xcalibur\methods
folder, and specify a file name.
16. Click
Save
.
