4
Daily Operation
Turning On the Reagent Ion Source
28
MS/ETD System Getting Started Guide
Thermo Scientific
4. Ensure that there is sufficient gas for the analysis.
Typical nitrogen gas consumption per day (when nitrogen is on 24 hours per day) is
between 5560 L (196.3 ft
3
) and 26700 L (943 ft
3
). If necessary, replace the tank. The
ETD module consumes less than 1 ATM cc/m of reagent carrier gas, so a standard large
bottle (245 ft
3
) of gas should last approximately two years.
For more information about gas requirements, refer to the
LTQ Series Preinstallation
Requirements Guide
.
Turning On the Reagent Ion Source
Even when the ETD module is on, the reagent ion source within it stays off until you turn
it on.
To turn on the reagent ion source
1. Open the Tune Plus window; see the instructions on
2. Choose
Setup > Reagent Ion Source
to open this dialog box (
Figure 18.
Reagent Ion Source dialog box
3. Select the
Reagent Ion Source On
check box.
4. Select the
Filament On
check box.
For tuning, you must manually select the Filament On check box.