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Introduction
Types of Experiments
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MS/ETD System Getting Started Guide
Thermo Scientific
LTQ XL and LTQ Velos Experiment Types
As described in the
LTQ Series Getting Started Guide
, the experiment templates available on all
LTQ XL and LTQ Velos mass spectrometers are as follows:
• General MS or MS
n
experiment
• Data-dependent experiments:
–
Data Dependent MS/MS
–
Data Dependent Triple Play
–
Nth Order Double Play
–
FAIMS Nth Order Double Play
–
Nth Order Triple Play
–
Data Dependent NL MS
3
–
Data Dependent product MS
3
–
Data Dependent Zoom Map
• Data Dependent Ion Tree
• Ion Mapping
ETD Experiment Types
With the addition of the ETD module to the LTQ XL or LTQ Velos MS detector, two other
experiment templates are available:
• Nth Order Double Play (ETD)—This experiment is useful as a survey experiment when
you cannot determine whether the CID or ETD mode of dissociation works best. For
example, CID works best for dissociating doubly-charged ions while ETD works best for
ions of a higher charge state.
• Data Dependent NL MS
3
(ETD)—Repeating the MS/MS experiment by using ETD
dissociation might make it easier to identify sites of the neutral loss (modification). ETD
dissociation is also useful when there are multiple sites of modification.
For additional information, see
