Thermo Scientific Dionex ICS-900, Installation And Quick Start Instructions

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Doc. 065214-02 1/12
17
Microbore systems: Use one or two red
backpressure coils (P/N 045878), supplied in
the microbore tubing kit (P/N 052324).
2. Remove the union connecting the green and
orange lines labeled
CELL OUT
(see Figure 31 ,
item ) and connect the backpressure coils.
Install the Suppressor
1. Install the hydrated suppressor onto the
brackets located below the conductivity cell
(see Figure 31 , item ).
2. Remove the union connecting
REGEN IN
and
REGEN OUT
(see Figure 31 , item ) and
connect these two lines to the corresponding
ports on the suppressor.
Fill the Regenerant Line
1. Turn on the pump and allow it to run until
liquid fills the
REGEN OUT
line and begins
entering the suppressor
REGEN IN
port. Turn
off the pump.
2. Remove the union connecting
ELUENT IN
and
ELUENT OUT
and connect these two lines to
the corresponding ports on the suppressor.
Equilibrate the System
1. Turn on the pump and run at the flow rate
recommended for the column.
2. Allow the system to equilibrate. During
equilibration, the Dionex ICS-900 Control
panel displays the background conductivity
(the conductivity of the eluent without the
offset performed by the autozero command).
3. Offset the background and zero the
conductivity reading by clicking the Autozero
button on the Control panel.
4. Monitor the system pressure to make sure it is
at the expected pressure for the installed
column (refer to the column manual for
details) and is stable. Column manuals
typically provide pressure specifications for
systems that do not include a guard column.
Expect the Dionex ICS-900 system pressure
(with a guard column) to be 15% to 20%
higher than the specification in the column
manual.
•
If the pressure is less than expected, air
may be trapped in the system. Release the
air by removing the pump fitting (
P ) on
the injection valve (see Figure 5 ). Allow
the air to escape and then reconnect the
fitting.
•
If the pressure is too high, there may be a
restriction in the system plumbing. Refer
to the Dionex ICS-900 operator’s manual
for troubleshooting information.
5. Check for leaks in the regenerant bottle.
6. Check that liquid is flowing out of the
suppressor
REGEN OUT
waste line.
7. Monitor the baseline conductivity. In general,
it should be <30

S for a system set up for
anion analyses, and <2

S for a system set up
for cation analyses. Equilibration time varies
and it can take some time to reach these
expected values.
Verify Operational Status
After the system has equilibrated, verify the actual
pump pressure and stability by monitoring the
pump pressure. Record the short-term pressure
fluctuations; they should be less than 0.13 MPa
(20 psi). If the pressure fluctuates by more than
this amount, prime the pump (see
page 14 ).
Flow Rate Number of Coils
0.3 to 0.5 mL/min 1 (red)
Less than 0.3 mL/min 2 (red)
4
5
6