Dionex ICS-900 Installation and Quick Start Instructions
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Doc. 065214-02 1/12
Prepare Eluent
1. Rinse the eluent bottle with deionized water.
2. Prepare 2 L of eluent. Refer to the instructions
in the column manual.
3. Fill the eluent bottle with the prepared eluent,
insert the stopper assembly tubing into the
bottle, and hand-tighten the cap.
4. Place the bottle in the tray on the top of the
Dionex ICS-900.
Prepare Regenerant
1. Verify that you have the correct regenerant
bottle assembly (P/N 068222 for anion
analyses; P/N 068223 for cation analyses):
•
For anions, verify that the cap is labeled
ANION
and the
REGEN BOTTLE OUT
line
extends to the bottom of the bottle.
•
For cations, verify that the cap is labeled
CATION
and the
REGEN BOTTLE OUT
line
extends only about 1 cm (0.4 in) into the
bottle.
2. Rinse the regenerant bottle with deionized
water.
3. Fill the bottle about halfway with deionized
water.
4. Determine the regenerant concentration
required for the application. See the following
guidelines; also refer to the Displacement
Chemical Regeneration (DCR) kit and column
manuals.
Guidelines for regenerant concentration
The optimal regenerant concentration can be
estimated for a known eluent strength by:
Anion Regenerant Concentration = (mM eluent) x 2
Cation Regenerant Concentration = (mM eluent) x 5
For example, if you are using 20 mM methane
sulfonic acid (MSA) as the eluent for cation
analysis, use a regenerant concentration of
100 mM tetrabutylammonium hydroxide
(TBAOH).
5. Empty the required amount of concentrate
(TBAOH for cations; sulfuric acid for anions)
into the bottle.
For acid concentrates (such as the
anion regenerant), always pour the
concentrate into deionized water, not
into the empty bottle.
Pour les concentrés acides (comme le
régénérant anionique), versez toujours
le concentré dans de l'eau désionisée
et non dans le réservoir vide.
Gießen Sie bei säurehaltigen
Konzentraten (beispielsweise dem
Kationenelutionsmittel) das Konzentrat
immer in entionisiertes Wasser und
nicht in den leeren Behälter.
6. Fill the bottle almost to the top with deionized
water and then place the bottle in the tray on
the top of the Dionex ICS-900.
NOTE To avoid staining the Dionex ICS-900, be
careful not to spill TBAOH on the instru-
ment.
7. Using the concentrate bottle, pour additional
deionized water into the bottle until it is
completely filled to the top. If a few drops
spill over, then it is full enough.
The regenerant bottle must be filled
all the way to the top at all times.
8. Insert the stopper assembly tubing into the
bottle and hand-tighten the cap.
9. Invert the bottle 3 or 4 times to disperse the
concentrate.
After analysis begins, do not mix the
contents of the regenerant bottle.
