B·R·A·H·M·S GmbH
B·R·A·H·M·S KRYPTOR compact PLUS
User Manual (Version R08en)
Page
8
of 30
Intended use
B.R.A.H.M.S KRYPTOR compact PLUS is a fully auto-
mated system for
in vitro
diagnostic. B.R.A.H.M.S
KRYPTOR compact is an autoanalyser which can
perform biochemical investigations.
It is used for measurement of patient samples in
random access mode routinely.
B.R.A.H.M.S KRYPTOR compact PLUS is a closed
system and can only operate utilising special reagents
offered by B·R·A·H·M·S GmbH. The system is based
on TRACE technology (Time-Resolved Amplified Cryp-
tate Emission). The system and the reagents are under
con tinuous develop ment by B·R·A·H·M·S GmbH.
TRACE – the unique measuring principle
of B.R.A.H.M.S KRYPTOR compact PLUS
The measurement principle of B.R.A.H.M.S KRYPTOR/
KRYPTOR compact is based on TRACE technology,
which measures the signal that is emitted from an
immuno complex with time delay.
The basis of the TRACE technology is non-radiative
energy transfer from a donor to an acceptor.
The proximity of donor and acceptor when they are
part of an immunocomplex and the spectral overlap
between donor emission and acceptor absorption
spectra on the one hand, intensify the fluorescent
signal of the cryptate and on the other hand they
extend the life span of the acceptor signal, permitting
the measure ment of temporally delayed fluorescence.
Precise measurement of analyte concentration
When the sample is excited with a nitrogen laser at
337 nm, the donor emits a long-life fluorescent signal
in the milli-second range at 620 nm, while the acceptor
generates a short-life signal in the nanosecond-range at
665 nm or 707 nm depending on the type of acceptor.
When the two components are bound in an immuno-
complex, both the signal amplifi cation and the prolong -
ed life span of the acceptor signal occur at 665 nm or
707 nm, so that it can be measured over µ-seconds.
This long-life signal is proportional to the concentration
of the analyte to be measured.
Introduction
Reliable prevention of interference
Non-specific signals are eliminated by the internally
calc ulated ratio of the intensities at these wavelengths
(665/620 or 707/620).
The signal generated by the cryptate at 620 nm serves
as an internal reference and is measured simul t -
ane ously with the long-life acceptor signal at 665 nm or
707 nm. Interfering influences,
e.g.
from turbid sera,
are automatically corrected.
Amplification
by energy
transfer
Donor
Bound
acceptor
Unbound acceptor
Life span of the
bound acceptor
signal / time-resolved
Life span of the
unbound acceptor signal
Unbound acceptor
Immunocomplex
Excitation
of the sample
at 337 nm
Excitation
of the sample
at 337 nm
10 nsec
µsec
Long-life
acceptor signal
at 665 or 707 nm
Time in µsec
Time in nsec
Intensity
Short-life
acceptor signal
at 665 or 707 nm
Intensity
0.5
620
665
λ
nm
1
Signal relation 665/620 = 1
Fluorescence intensity
Donor
Acceptor
0.5
620
665
λ
nm
1
Signal relation 665/620 = 1
Fluorescence intensity
Donor
Acceptor
T = 100%
T = 50%
Internal correction of interfering influences
