Thermo Scientific BIOLiner Megafuge 40 Instruction Manual Download Page 1

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Thermo Scientific

BIOLiner™

Instruction Manual

50119954-

• 0

/ 2020

Summary of Contents for BIOLiner Megafuge 40

Page 1: ...Visit us online to register your warranty thermofisher com labwarranty Thermo Scientific BIOLiner Instruction Manual 50119954 e 07 2020 ...

Page 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

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Page 5: ...4 3 Maximum Loading 4 3 Cycle Counter 4 3 Chapter 5 Aerosol tight Applications 5 1 Basic Principles 5 2 Fill Level 5 2 Checking the aerosol Tightness 5 2 Table of Contents Foreword iii Scope of Supply iii Precautions iii Autoclaving 6 5 Service of Thermo Fisher Scientific 6 5 RCF Values A 1 RCF Values BIOLiner bucket A 1 RCF Values Microplate Carrier A 2 Chemical Compatibility Chart B 1 Chapter 6 ...

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Page 7: ...warranty Scope of Supply If any parts are missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the BIOLiner the following general safety regulations must be followed Do not use rotors which show any signs of corrosion and or cracks Do not touch the electronic components of the rotor and do not make any changes to the electronic...

Page 8: ...y particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 1 2 g cm 3 This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symb...

Page 9: ...Thermo Scientific 75003667 BIOLiner 1 1 1 Rotor Specifications Contents Technical Data on page 1 2 ...

Page 10: ...ure of 23 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R Catalog 75004515 75004536 75004518 Weight empty kg 7 3 7 3 7 3 Max cycle number 55000 55000 55000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 350...

Page 11: ...3 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R Catalog 75004515 75004536 75004518 Weight empty kg 7 3 7 3 7 3 Max cycle number 90000 90000 90000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 3...

Page 12: ... 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R Catalog 75004516 75004537 75004519 Weight empty kg 7 3 7 3 7 3 Max cycle number 55000 55000 55000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 35...

Page 13: ... 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R Catalog 75004516 75004537 75004519 Weight empty kg 7 3 7 3 7 3 Max cycle number 90000 90000 90000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 35...

Page 14: ...3 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004520 75004538 75004524 Weight empty kg 7 3 7 3 7 3 Max cycle number 55000 55000 55000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 3...

Page 15: ...3 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004520 75004538 75004524 Weight empty kg 7 3 7 3 7 3 Max cycle number 90000 90000 90000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 3...

Page 16: ... 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004521 75004539 75004525 Weight empty kg 7 3 7 3 7 3 Max cycle number 55000 55000 55000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 35...

Page 17: ... 25 C running time 120 minutes 8 8 8 Aerosol tight yes yes yes Permissible temperature range autoclavable cycle number C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004521 75004539 75004525 Weight empty kg 7 3 7 3 7 3 Max cycle number 90000 90000 90000 Maximum permissible load g 4 x 750 4 x 750 4 x 750 Maximum speed nmax rpm 35...

Page 18: ...mperature of 23 25 C running time 120 minutes 8 8 Aerosol tight yes yes Permissible temperature range autoclavable cycle number C 121 121 Tested by HPA Porton down UK Centrifuge Sorvall Legend XTR Sorvall Legend XFR Catalog 75004523 75004541 Weight empty kg 7 3 7 3 Max cycle number 55000 55000 Maximum permissible load g 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 Maximum RCF value at nmax 273...

Page 19: ...ad g Maximum speed nmax rpm Maximum RCF value at nmax Max min radius cm Pitch angle Accel braking time s Aerosol tight Permissible temperature range autoclavable cycle number C Tested by HPA Porton down UK Thermo Scientific 75003667 BIOLiner 1 11 Sorvall Legend XT Sorvall Legend XF Sorvall ST 40 75004507 75004534 75004511 7 3 7 3 7 3 90000 90000 90000 4 x 500 4 x 500 4 x 500 3500 3500 3500 2739 27...

Page 20: ...utes 8 8 Aerosol tight yes yes Permissible temperature range autoclavable cycle number C 121 121 Tested by HPA Porton down UK Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR Catalog 75004527 75004543 Weight empty kg 7 3 7 3 Max cycle number 55000 55000 Maximum permissible load g 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 Maximum RCF value at nmax 2739 2739 Max min radius cm 20...

Page 21: ...utes 8 8 Aerosol tight yes yes Permissible temperature range autoclavable cycle number C 121 121 Tested by HPA Porton down UK Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR Catalog 75004527 75004543 Weight empty kg 7 3 7 3 Max cycle number 90000 90000 Maximum permissible load g 4 x 750 4 x 750 Maximum speed nmax rpm 3500 3500 Maximum RCF value at nmax 2451 2451 Max min radius cm 17...

Page 22: ...time 120 minutes 8 Aerosol tight yes Permissible temperature range autoclavable cycle number C 121 Tested by HPA Porton down UK Centrifuge Thermo Scientific SL 40R Catalog 75004528 Weight empty kg 7 3 Max cycle number 55000 Maximum permissible load g 4 x 750 Maximum speed nmax rpm 3500 Maximum RCF value at nmax 2739 Max min radius cm 20 10 5 Pitch angle 90 Accel braking time s 55 50 Minimum temper...

Page 23: ...time 120 minutes 8 Aerosol tight yes Permissible temperature range autoclavable cycle number C 121 Tested by HPA Porton down UK Centrifuge Thermo Scientific SL 40R Catalog 75004528 Weight empty kg 7 3 Max cycle number 90000 Maximum permissible load g 4 x 750 Maximum speed nmax rpm 3500 Maximum RCF value at nmax 2451 Max min radius cm 17 9 10 5 Pitch angle 90 Accel braking time s 55 50 Minimum temp...

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Page 25: ...Thermo Scientific 75003667 BIOLiner 2 1 2 Accessories Contents Rotor data on page 2 2 Accessories on page 2 2 Bottle 75003699 on page 2 3 ...

Page 26: ...91 4 1 3141 0250 250 250 PP Oak Ridge Bottle 4 3500 62 x 130 Included 4 PP Sealing 75003675 4 2 3140 0250 250 250 PC OakRidge Bottle 4 3500 62 x 130 Included 4 PP Sealing 75003675 4 2 225 225 BD Conical 3500 62 x 130 75003675 BD Cusion 352090 4 2 376813 200 200 Nunc Conical 4 3500 62 x 130 Included 4 PP Sealing 75003675 Nunc Cushion 377585 4 2 175 175 BD Conical 3500 62 x 130 75003675 BD Cusion 35...

Page 27: ...tation from the upper edge The bottle should be filled to min 80 The top is a single piece Close the filled bottle and tighten the top firmly Weigh the bottles opposite in order to avoid an imbalance Refer to the rotor loading chapter CAUTION The bottle 75003699 is a wearing product Bottle with signs of wear are no longer to be used Replace the bottles Damaged bottles can cause damage to the rotor...

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Page 29: ...Thermo Scientific 75003667 BIOLiner 3 1 3 AutoLock Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 3 ...

Page 30: ...e spindle The rotor clicks automatically into place 3 Check if the rotor is properly installed by lifting slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then ...

Page 31: ...ands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this Figure 3 2 Handling of AutoLock Aerosol tight Rotors CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling its handle Be sure to check all seals before starting any aerosol tight applications CAUTION Rotors supplied with a lid for aerosol tight applications ...

Page 32: ...3 AutoLock Aerosol tight Rotors 3 4 75003667 BIOLiner Thermo Scientific Figure 3 3 AutoLock lid for aerosol tight rotors WARNING Mind the AutoLock mandril inside the lid Do not touch ...

Page 33: ...Thermo Scientific 75003667 BIOLiner 4 1 4 Rotor Loading Contents Before a Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 Cycle Counter on page 4 3 ...

Page 34: ...cket caps 6 Check the rotor bolts and apply grease 75003786 before using for the first time 7 Check that each bucket or microplate carrier can swing freely by moving it carefully with your hand 8 Weigh the bucket content adapter and tube Be sure not to overload the rotor while doing this Proper Loading To ensure safe operation of the centrifuge the rotor must be evenly loaded at all times You can ...

Page 35: ...stability even when spinning at top speed The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adapter exceeds the maximum permissible load Reduce the fill level Recalculate the maximum permissible speed according to the following formula and select the calculated maximum speed for the centri...

Page 36: ...4 Rotor Loading Cycle Counter 4 4 75003667 BIOLiner Thermo Scientific Service life examples Usage profile Maximum service life at 50000 cycles frequent use 20 runs day 220 days year 11 years ...

Page 37: ...Thermo Scientific 75003667 BIOLiner 5 1 5 Aerosol tight Applications Contents Basic Principles on page 5 2 Fill Level on page 5 2 Checking the aerosol Tightness on page 5 2 ...

Page 38: ... rotor is aerosol tight depends primarily on proper handling Check as needed to make sure your rotor is aerosol tight The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks scratches and embrittlement is extremely important Aerosol tight applications are not possible if the vessel tops are open Aerosol tightness requires the correct operation when filling...

Page 39: ...tected by escaping water or the sound of escaping gas Replace the seals if you detect any leaks Then repeat the test 5 Dry the rotor rotor cover and the cover seal Aerosol tight Closure with ClickSeal 1 If necessary grease the lid joint before closing the lid Use grease 76003500 for this 2 Raise the latch The cap can now be easily placed on the bucket Figure 5 4 Bucket with open lid 3 Lower the la...

Page 40: ... 75003667 BIOLiner Thermo Scientific Figure 5 5 Bucket with closed lid CAUTION If the latch is not flipped down the caps could be damaged during centrifuging If the latch has not clicked into place the bucket is not aerosol tight Never raise the bucket at its latch ...

Page 41: ...667 BIOLiner 6 1 6 Maintenance and Care Contents Cleaning Intervals on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 5 ...

Page 42: ... parts Use only disinfectants with a pH of 6 8 Dry aluminium parts off with a soft cloth After cleaning treat the entire surface of aluminium parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminium parts at room temperature or in a cold storage room with the cavities pointing down Maintenance Recommended interval Clean rotor chamber Daily or when polluted ...

Page 43: ...us material has spilled during centrifugation Use a sprayer whenever possible so that all surfaces are covered evenly The rotor chamber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered evenly CAUTION When cleaning do not allow liquids especially organic solvents t...

Page 44: ...eed be CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 4 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle 5 Remove the centrifuge tu...

Page 45: ...ers inspection and service contracts for this work Note No chemical additives are permitted in the steam CAUTION Never exceed the permitted temperature and duration when autoclaving If the rotor shows signs of corrosion or wear it must be replaced 4 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle 5 Remove the centrifuge tubes and adapters and dispose of them or di...

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Page 47: ...181 1000 10 5 20 117 224 1100 10 5 20 142 271 1200 10 5 20 169 322 1300 10 5 20 198 378 1400 10 5 20 230 438 1500 10 5 20 264 503 1600 10 5 20 301 572 1700 10 5 20 339 646 1800 10 5 20 380 724 1900 10 5 20 424 807 2000 10 5 20 470 894 2100 10 5 20 518 986 2200 10 5 20 568 1082 2300 10 5 20 621 1183 2400 10 5 20 676 1288 2500 10 5 20 734 1398 2600 10 5 20 794 1512 2700 10 5 20 856 1630 2800 10 5 20...

Page 48: ...8 900 10 5 17 9 95 162 1000 10 5 17 9 117 200 1100 10 5 17 9 142 242 1200 10 5 17 9 169 288 1300 10 5 17 9 198 338 1400 10 5 17 9 230 392 1500 10 5 17 9 264 450 1600 10 5 17 9 301 512 1700 10 5 17 9 339 578 1800 10 5 17 9 380 648 1900 10 5 17 9 424 722 2000 10 5 17 9 470 800 2100 10 5 17 9 518 883 2200 10 5 17 9 568 969 2300 10 5 17 9 621 1059 2400 10 5 17 9 676 1153 2500 10 5 17 9 734 1251 2600 1...

Page 49: ...A RCF Values Thermo Scientific 75003667 BIOLiner A 3 3200 10 5 17 9 1202 2049 3300 10 5 17 9 1278 2179 3400 10 5 17 9 1357 2313 3500 10 5 17 9 1438 2451 Speed rpm Min radius Max radius RCF Rmin RCF Rmax ...

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Page 51: ... M S S Aluminium Chloride U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S ...

Page 52: ...U S M U U U M M U S S U U Diethylpyrocarbonate S S U S S S S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S...

Page 53: ... 5 U U S S S S M S S S S S U S S S S S S S M U M S U Potassium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S...

Page 54: ...S S Sodium Sulfide S S S S S S S U U S S S M S Sodium Sulfite S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S S S S M S S S Oils Petroleum S S S S U S S S S U U M S M U U S S S U S S S S Oils Other S S S M S S S S U S S S S U S S S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S ...

Page 55: ...S S S M S S S S S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U S S U M S U S U U S M M S S U U S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buff...

Page 56: ... COATING for ALUMINIUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy DELERIN ETHYLENE PROPYLENE GLASS NEOPRENE NORYL NYLON PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON A TEFLON SILICONE RUBBER STAINLESS STEEL TITANIUM TYGON VITON Key S ...

Page 57: ...Chemical Compatibility Chart B 1 Cleaning 6 2 Cleaning Intervals 6 2 Cycle Counter 4 3 D Decontamination 6 4 Disinfection 6 3 F Fill Level 5 2 Foreword iii I Improper Loading 4 3 M Maintenance and Care 6 1 Maximum Loading 4 3 P Precautions iii Proper Loading 4 2 R RCF Values A 1 RCF Values BIOLiner bucket A 1 RCF Values Microplate Carrier A 2 Removing the Rotor 3 3 Rotor Installation 3 2 Rotor Loa...

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Page 59: ...s terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium ...

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