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Thermo Scientific 75004559, Instruction Manual

The Thermo Scientific 75004559 Instruction Manual is a comprehensive guide to help you set up and operate your product efficiently. This essential manual is available for free download at our website, ensuring easy access to all the necessary information you need. Maximize the potential of your product with this user-friendly manual.

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A

  Chemical Compatibility Chart

A-6

 

Heraeus Multifuge X3 F / X3 FR

Thermo Scientific

Chemical resistance data is included only as a guide to product use. No organized chemical resistance 

data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting 

sample lots. 

Hydrogen Peroxide (10%)

U U M S

S

U U -

S

S

S

U S

S

S

M U S

S

S

S

S

S

M S

U S

Hydrogen Peroxide (3%)

S

M S

S

S

-

S

-

S

S

S

S

S

S

S

S

M S

S

S

S

S

S

S

S

S

S

Xylene

S

S

U S

S

S

M U S

U U U U U U M U M U U U S

U M S

U S

Zinc Chloride

U U S

S

S

S

U S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

U S

S

S

Zinc Sulfate

U S

S

-

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

S

Citric Acid (10%)

M S

S

M S

S

M S

S

S

S

S

S

S

S

S

M S

S

S

S

S

S

S

S

S

S

1

Polyethyleneterephthalate

CHEMICAL

MA

TER

IAL

AL

UMINUM

ANODIC COA

TING f

or

 AL

UMI

N

U

M

BU

NA

 N

CE

LL

UL

OSE A

C

ET

A

TE

 BUTYRA

TE

POL

YURETH

ANE ROTO

R 

PA

INT

CO

MPO

S

IT

E Car

bon

 Fi

ber

/Ep

ox

y

DEL

RIN

®

ETHYL

ENE P

R

O

PYLE

N

E

GL

ASS

N

EOPRENE

NO

RY

L

®

NY

LO

N

PET

1

, P

O

LY

C

LEA

R

®

,CL

EARC

R

IMP

®

C

CCLEARCR

IM

P

®

POL

YA

LL

O

ME

R

POL

YCARB

ON

A

TE

POL

YESTER, G

LA

S

S T

HERMOSET

POL

YTHE

RM

IDE

POL

YRTHYL

ENE

POL

YPROPYL

ENE

POL

YSULF

ONE

POL

YVINY

L CHLORIDE

RU

LO

N A

®

, T

EFLO

N

®

SI

LI

C

O

N

E RU

BB

ER

S

TAINL

ES

S STEEL

TIT

A

NIU

M

TYGON

®

VI

TON

®

Key 

S  

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.

M

Suggest testing under actual conditions of use. 

U

Unsatisfactory, not recommended.

--

Performance unknown; suggest testing, using sample to avoid loss of valuable material.

Summary of Contents for 75004559

Page 1: ...Thermo Scientific Heraeus Multifuge X3F X3FR Centrifuge Instruction Manual 50156635 b 03 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Page 2: ......

Page 3: ...4 Mains Supply 1 4 Rotor Selection 1 5 Before use 2 1 Before Setting up 2 2 Setting up 2 2 Transporting the Centrifuge 2 4 Location 2 4 Aligning the Centrifuge 2 4 Mains Connection 2 5 Storage 2 6 Control Panel 3 1 Control Panel 3 2 Keys 3 2 Operation 4 1 Switch on Centrifuge 4 2 Lid Opening 4 2 Close Lid 4 2 Rotor Installation 4 2 Entering Parameters 4 4 Centrifugation 4 10 Temperature Adaptation...

Page 4: ... 5 2 Maintenance and Care 6 1 Cleaning Intervalls 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fisher Scientific 6 5 Trouble Shooting 7 1 Mechanical Emergency Door Release 7 2 Troubleshooting by User 7 3 When to contact Customer Service 7 5 Chemical Compatibility Chart A 1 ...

Page 5: ...e to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative Intended Use This centrifuge is designed to separate sample mixtures of different densities like chemicals environmental samples and other non human body samp...

Page 6: ...ntrifuge is to be used for its intended use only Plug the centrifuge only into sockets which have been properly grounded Do not move the centrifuge while it is running Do not lean on the centrifuge Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided the...

Page 7: ... hazardous substances Do not centrifuge explosive or flammable materials or substances which could react violently with one another The centrifuge is neither inert nor protected against explosion Never use the centrifuge in an explosion prone environment Do not centrifuge inflammable substances Do not centrifuge toxic or radioactive materials or any pathogenic micro organisms without suitable safe...

Page 8: ...o general hazards CAUTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe ...

Page 9: ...duction and Description Contents Characteristics of the Heraeus Multifuge X3F X3FR on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 4 Functions and Features on page 1 5 Mains Supply on page 1 5 Rotor Selection on page 1 6 ...

Page 10: ...nd RCF or the entry mode These settings can be changed even while the centrifuge is running you can also centri With the help of the PULSE key fuge a sample for just a few seconds if called for The Heraeus Multifuge X3 F X3 FR is equipped with various safety features The housing and rotor chamber consist of steel plate the interior of armour steel while the front panel is made of high impact resis...

Page 11: ...rated 230 V 120 V 230 V 120 V 6653 BTU h 4776 BTU h 5800 BTU h 4776 BTU h IP 20 20 running time unlimited unlimited max Speed nmax 15200 rpm depending on the rotor 15200 rpm depending on the rotor min Speed nmin 300 rpm 300 rpm maximum RCF value at nmax 25314 x g 25314 x g maximum kinetic energy 62 5 kJ 62 5 kJ noise level at maximum speed 64 dB A 64 dB A temperature setting range 10 C to 40 C Dim...

Page 12: ...brushes Drive Easy to clean keypad and display surface Keys and display Controls Microprocessor controlled The most recent data is saved Internal memory Functions RCF selection temperature control pretemp with cooling equipped devices 9 acceleration and 10 braking curves Acceleration braking profiles Automatic Rotor recognition Electronic contingent on rotor and speed Imbalance recognition Automat...

Page 13: ...g manuals For more information visit our website at http www thermofisher com TX 750 75003180 with round buckets 75003608 with rectangular buckets 75003614 with Microplate carrier 75003617 BIOshieldTM 1000A 75003182 HIGHPlateTM 6000 75003606 Fiberlite H3 LV 75003665 Fiberlite F14 6 x 250 LE 75003662 Fiberlite F15 6 x 100y 75003698 Fiberlite F13 14 x 50cy 75003661 Fiberlite F15 8 x 50cy 75003663 Fi...

Page 14: ...eus Multifuge X3F X3FR 2 1 2 Before use Contents Before Setting up on page 2 2 Transporting the Centrifuge on page 2 4 Location on page 2 4 Aligning the Centrifuge on page 2 4 Mains Connection on page 2 5 Storage on page 2 6 ...

Page 15: ...ientific immediately if any damage is discovered 2 Remove the packaging 3 Check the order for completeness see Scope of Supply on page iii If the order is incomplete please contact Thermo Fisher Scientific Setting up 1 Place the pallet with the centrifuge so that you have at leased 1 5 m room in front of the pallet 2 Release the rails from the pallet 3 Screw the rails onto the pallet in front of t...

Page 16: ...Heraeus Multifuge X3F X3FR 2 3 4 Remove the protectors that locate the centrifuge on the pallet Release the castors Use several people in order to roll the centrifuge off the pallet 5 Fix the castors once the centrifuge is in its final position ...

Page 17: ...e and free of resonance for example a level laboratory bench The supporting structure must be suitable for horizontal setup of the centrifuge The centrifuge should not be exposed to heat and strong sunlight The set up location must be well ventilated at all times Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location Th...

Page 18: ...switch on the back press 0 2 Plug the centrifuge into grounded electrical sockets only 3 Check whether the cable complies with the safety standards of your country 4 Make sure that the voltage and frequency correspond to the figures on the rating plate 5 Establish the connection to the power supply with the connecting cable Main Power Switch Mains plug ...

Page 19: ...entific Storage Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid direct sunlight Fix the castors ...

Page 20: ...Thermo Scientific Heraeus Multifuge X3F X3FR 3 1 3 Control Panel Contents Control Panel on page 3 2 Keys on page 3 2 ...

Page 21: ...mple temperature Key Display contents Start Normal start of the centrifuge Stop End run manually Open lid Automatic release possible only when device is switched on Emergency release see Mechanical Emergency Door Release on page 7 2 Pulse By pressing the key PULSE the centrifuge starts immediately and accelerates up to the end speed Releasing the key initiates a stopping process at the highest bra...

Page 22: ...e on page 4 2 Lid Opening on page 4 2 Rotor Installation on page 4 2 Entering Parameters on page 4 4 Centrifugation on page 4 10 Temperature Adaptation during Standstill on page 4 11 Short term Centrifugation on page 4 11 Removing the Rotor on page 4 12 Aligning the Centrifuge on page 4 13 ...

Page 23: ...ay shows The top line shows the message Lid open The bottom line shows the values for the last run Lid Opening 1 Press the OPEN key Close Lid 2 Close the lid by pressing down on it lightly in the middle or on both sides of it Two locks close the lid completely Rotor Installation The approved rotors for the Heraeus Multifuge X3 F X3 FR are listed in section Rotor Selection on page 1 6 Use only the ...

Page 24: ...rotor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with...

Page 25: ... by entering a digit between 1 9 on the numeric pad Curve number 1 is the slowest and curve number 9 the fastest 3 Confirm your entry by pressing ENTER Braking curve 1 Press the ACC DEC key twice in order to open the braking curve selection menu The display shows the message Set deceleration 2 Select the desired curve by entering a digit between 1 9 on the numeric pad Curve number 1 is the slowest...

Page 26: ...omatically if the resulting speed is less than 300 rpm This is because 300 rpm is the lowest selectable speed Explanation of RCF Value The relative centrifugal force is given as a multiple of the force of gravity g It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices since it is independent of the type of device Only the centrifuging...

Page 27: ...inue running until you stop it manually with the STOP key Run time mode Use the TOGGLE key to set the run time counter start AT START When AT START is lit the running time will be counted right from pressing the START key AT SPEED When AT SPEED is lit the running time will be counted once the preset speed or the preset RCF value respectively have been reached In this case the selected acceleration...

Page 28: ...des 2 Enter the desired temperature using the numeric pad The digits show in sequential order 3 Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds Prewarming or precooling the centrifuge For setting the pretemp value for the centrifuge proceed as follows 1 Hold down the TEMPERATURE key for a minimum of three seconds to open the...

Page 29: ...t four digits of the bucket catalog number 1 Press the BUCKET key The display shows the following message 2 Press the BUCKET key repeatedly until the bucket being used is displayed Your entry will be automatically confirmed if you do not press any key for 5 seconds Bucket radius Having entered the bucket code you may want to specify the radius When you use adaptors you will have to specify the rad...

Page 30: ...ecting a program Programs can be stored and retrieved later by means of the assigned number 1 Enter the program parameters as described above 2 Press the program memory key to load any of the programs 1 5 Press the DIRECTORY key in order to load any of the programs 1 99 by means of the numeric pad Enter the program number and confirm with ENTER key 3 You may want to enter a passcode to protect the...

Page 31: ...n two hours ago this message will apear in the display Check the castor breaks before pressing the START key again Starting centrifuge program Press the START key on the control panel The centrifuge accelerates to the pre set speed with the time display active If the speed setting is higher than the maximum permissible speed or RCF value for the particular rotor then the display will show the mess...

Page 32: ...erated at the designated rate The message END will illuminate and after pressing the key OPEN the lid will open and you can remove the centrifuged material Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified For this the centrifuge must have accelerated to over 500 rpm The speed display will then show END When the rotor is not r...

Page 33: ... centrifuge spindle Make sure not to tilt the rotor while doing this Aerosol tight Rotors When using an aerosol tight lid the rotor can only be removed with the lid closed This is to protect you and the samples TM Figure 4 2 Auto Lock lid for aerosol tight rotors CAUTION Rotors supplied with a lid for aerosol tight applications come with a mandrel which belongs to the Auto LockTM Be sure not to pl...

Page 34: ...4 Operation Aligning the Centrifuge Thermo Scientific Heraeus Multifuge X3F X3FR 4 13 Aligning the Centrifuge 1 To turn off the centrifuge put the mains switch to 0 ...

Page 35: ... 1 7 Deutsch English 1 Deutsch 2 Française 3 Español 4 Italiano 5 Nederlands 6 7 Росский STOP ENTER LCD powersave START no 0 yes 1 ENTER ENTER No Program passcodes 0547V01 0 1 1 Software and identification NVRAM example display 4641V05 1 ENTER STOP ENTER Set lid passcode ENTER No Auto lid open ENTER ENTER 0547V01 Software ID 2368 Cycle count ENTER ...

Page 36: ...r the run Otherwise press 0 no Press ENTER key to confirm Keypad beep Press 1 yes on the numeric pad if the centrifuge should make beep when pressing any key Otherwise press 0 no Press ENTER key to confirm LCD powersave Press 1 yes on the numeric pad if the centrifuge should enter a powdersave mode after the run Otherwise press 0 no Press ENTER key to confirm Lid passcode Press 1 yes on the numeri...

Page 37: ...n Press 1 yes on the numeric pad if the centrifuge should open after the run Otherwise press 0 no Press ENTER key to confirm Software ID Here you fine the current software version Press ENTER key to confirm Cycle count Here you fine the current numbers of cycles Press ENTER key to confirm ...

Page 38: ...ltifuge X3F X3FR 6 1 6 Maintenance and Care Contents Cleaning Intervalls on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 5 ...

Page 39: ... must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with...

Page 40: ...d during centrifugation The rotor chamber and the rotor should be treated preferably with a neutral disinfectant CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment CAUTION When cleaning do not allow liquids especially organic solvents to get on the ...

Page 41: ...r cabinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Grease the bolt of the swing out rotor with bolt grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactive decontamina...

Page 42: ...aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Grease the bolt of the swing out rotor with bolt grease 75003786 Autoclaving 1 Before autoclaving clean rotor and accessories and described above 2 Place the rotor on a flat surface Rotors and adapter can beautoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Service of Thermo Fi...

Page 43: ...Scientific Thermo Fisher Scientific offers inspection and service contracts for this work Any necessary repairs are performed for free during the warranty period and afterwards for a charge This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician ...

Page 44: ...hermo Scientific Heraeus Multifuge X3F X3FR 7 1 7 Trouble Shooting Contents Mechanical Emergency Door Release on page 7 2 Troubleshooting by User on page 7 3 When to contact Customer Service on page 7 5 ...

Page 45: ... braking process lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the lid 2 Pull out the power supply plug 3 On the back of the housing are two black plastic plugs which you can pry out of the back plate with a screwdriver or a knife Pull the release cord attached to it at the same time to trigger the mechanical lid release The lid will open and the sa...

Page 46: ...ng braked Bucket code is undefined for the rotor detected check the set points for the given bucket code Is it permitted to use the current bucket in the rotor currently mounted Restart the centrifuge If an error message appears again inform Customer Service The centrifuge cannot be operated Unapproved rotor The run does not start or the centrifuge runs down without being braked Rotor code is not ...

Page 47: ...ntrifuge If an error message appears again inform Customer Service The centrifuge cannot be operated E 13 The run does not start or the centrifuge runs down without being braked The check sum in the data memory is incorrect The software corrects errors automatically Check the values of the set point settings etc Restart the centrifuge If an error message appears again inform Customer Service Tempe...

Page 48: ...the centrifuge If an error message appears again inform Customer Service E 33 The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked Overpressure in the refrigeration unit Clean the air inlet for the condenser Restart the centrifuge If an error message appears again inform Customer Service E 34 E 36 The centrifuge cannot be operated The run does n...

Page 49: ... contact Customer Service 7 6 Heraeus Multifuge X3F X3FR Thermo Scientific 3 Press and hold the ENTER key until the following message is displayed 4 Communicate the software version to the service technician Software ID XXXXXXXX ...

Page 50: ...Allyl Alcohol U S S S S M S S S M S S Aluminum Chloride U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U ...

Page 51: ... Diethyl Ether S S U U S S S U S U U S U U U U U U U U U S S S S M U Diethyl Ketone S U U M S U S M U U U M M U S S U U Diethylpyrocarbonate S S U S S S S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S...

Page 52: ...S S M S S S Potassium Carbonate M U S S S S S S S S S S S U S S S S S S S S S S S S Potassium Chloride U S S S S S S S S S S S S S S S S S S S S U S S S Potassium Hydroxide 5 U U S S S S M S S S S S U S S S S S S S M U M S U Potassium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S ...

Page 53: ...ite 5 U U M S S M U S S M S S S M S S S S M S S S M U S M S Sodium Iodide M S S S S S S S S S S S S S S S S S S M S S S Sodium Nitrate S S S S S S S S S S S S S S S S S S S S U S S S S Sodium Sulfate U S S S S S S S S S S S S S S S S S S S S S M S S S Sodium Sulfide S S S S S S S U U S S S M S Sodium Sulfite S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S...

Page 54: ...ric Acid 50 U U U U S U U S M S U U M U U S S S S M S M U U M M Sulfuric Acid 10 M U U S S U U S S M U S S S S S S S S S S U U U S S Sulfuric Acid 50 M U U U S U U S S M U U S U U M S S S S S U U U M S Sulfuric Acid conc M U U U U U M M U U S U U U M S U M S U U U S Stearic Acid S S S M S S S S S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U Toluene ...

Page 55: ...S S S S Citric Acid 10 M S S M S S M S S S S S S S S S M S S S S S S S S S S 1Polyethyleneterephthalate CHEMICAL MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy DELRIN ETHYLENE PROPYLENE GLASS NEOPRENE NORYL NYLON PET 1 POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHER...

Page 56: ...anation of RCF Value 4 5 F Failure message 7 3 Features 1 5 I Imbalance indicator 4 10 Intended Use v K Keys 3 2 Kundendienst 6 5 7 5 L Lid Opening 4 2 Location 2 4 M Mains Connection 2 5 Mains Supply 1 5 2 6 Maintenance 6 1 O Operation 4 1 Overwriting a passcode protected program 4 9 Overwriting a program 4 9 P Passcode 4 9 Precautions iv Preface iii Preselecting RCF 4 4 Preselecting Speed 4 4 Pr...

Page 57: ... 2 4 Select Program 4 9 Short term Centrifugation 4 11 Starting centrifuge program 4 10 Stopping the centrifugation program 4 11 Storage 2 6 Switch on Centrifuge 4 2 T Technical Data 1 3 Temperature Adaptation 4 11 Transporting the Centrifuge 2 4 Trouble Shooting 7 1 ...

Page 58: ... Not all products are available in all countries Please consult your local sales represen tative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Italy 39 02 95059 552 Japan 81 3 5826 1616 Korea 82 2 2023 0600 Netherlands 31 76 579 55 55 Ne...

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