8. Press and hold the prime key
on the drive console to prime the
pump. Priming will stop when key is released.
9. Place a measuring container at the pump outlet. Highlight the START
field and press the ENTER key. The drive will run based on the
default volume at the estimated flow rate selected.
10. Upon completion of the calibration run period, the CAL VOLUME
field will be highlighted. Press the ENTER key and adjust the CAL
VOLUME to the measured quantity. Use the LEFT/RIGHT keys to
select digit to be changed, use the UP/DOWN keys to adjust the
value, and press ENTER to SAVE setting and EXIT the field.
A lower case “c” should now be displayed when the calibrated tubing
size is selected. The volume units will depend on the flow rate units.
The flow rate unit mL/min will result in a volume unit of mL; oz/min
will result in a volume unit of oz.
Tubing Calibration Notes
•
If the drive is stopped during calibration, empty the container and
re-start the procedure.
•
Calibration time at maximum allowable flow rate (default max
flow rate) is 15 seconds and at minimum allowable flow rate
(approximately 6% of the maximum flow rate) is 4 minutes.
Select the CUSTOM tube size for other tubing sizes or lower
flow rates.
•
Minimum and maximum flow rates will change after a tubing
calibration due to a re-calculation of the vol/rev.
•
Optimum results are best obtained after tubing has been broken in
by running in pump for at least 10 minutes. Steps 7-10 can be
repeated as necessary to optimize the accuracy of the tubing cal.
CAL RUN TIME FORMULA
60 / (flow rate [mL/min] / cal volume [mL]) = cal run time (seconds)
INVALID CAL RUN TIME EXAMPLE
•
tube size 13 flow rate range is 0.4 mL/min – 40.0 mL/min
•
at flow rate of 1 mL/min, cal run time calculation is as follows:
60 / (1 mL/min / 10 mL) = 600 seconds
600 seconds exceed the max run time of 4 minutes (240 seconds)
NOTE:
The minimum pump speed for preforming a tubing calibration is
25 rpm. Flow rate equivalents below 25 rpm will be reset to 25 rpm for
the tubing calibration.
Tubing Calibration
(continued)
Section 3
Operation
Thermo Scientific
FH100D/FH100DX Dispensing Pump Operating Manual 3-5
