SM-BioIIAdvancePlus-EN-1812
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5.5.
CALIBRATION OF THE CABINET
The purpose of this technical instruction is to regulate and check that the impulsion speed
flow and inflow are suitable to achieve product and user protection. To determine the speed
average as well as the uniformity range in the laminar flow zone and velocity through the
front aperture of the microbiological safety cabinet.
INSTRUMENTATION
•
Omnidirectional anemometer with a support.
•
Unidirectional anemometer.
•
Meter, calculator, permanent felt-tip pen or squares template.
METHODOLOGY
1.
Switch on the cabinet (the front glass should be in the working position and should not
be any alarm except the laminar flow or extraction).
2.
In the upper side open the extraction regulator flow as shown in the pictures below. (Bio-
II-Advance 6 do not assembly these parts)
Bio-II-Advance 3
Bio-II-Advance 4
Bio-II-Advance 6
3.
Go to Technical service Menu.
4.
Select size model (3,4,6). Adjust the set point extraction (0.55 m/s in Advance 3 & 4;
0.50 m/s in Advance 6) and adjust the impulsion set point to 0.35 m/s.
5.
Go to calibration menu
6.
Waiting at least 2 minutes. The cabinet is working now at constant fans voltage.
7.
Take 5 velocity readings at 2.5 cm over the protective grills or at the level of the slot of
the exhaust, as indicated below. Calculate and enter the average inflow velocity:
V
average
(m/s)= 1/5 x (V
1
+ V
2
+ V
3
+ V
4
+ V
5
)
Q
exhaust
(m
3
/h)= (V
average
x Exhaust surface) x 3600
NOTE: Exhaust surface: 0.078 m
2
.
V
inflow
(m/s)= Q
exhaust
/ (Front Aperture surface x 3600)
NOTE: Inflow surfaces:
Bio II Advance 3: 0.149 m
2
Bio II Advance 4: 0.203 m
2
Bio II Advance 6: 0.313 m
2
Unidirectional anemometer exhaust position
8.
Then, measure the impulsion flow. (8 points in Bio-II-Advance 3, 8 points in Bio-II-
Advance 4 and 12 points in Bio-II-Advance 6, see calibration pattern in Annex A9.3 of
the User’s Manual to see the reading point situation and order for each model).
