Operating Manual
M01534_v1.1 MagicPrep NGS Instrument Operating Manual
Page 34 of 39
Problem / Error
Possible Cause
Corrective Measure
If the error still exists, please contact Tecan
Customer Support.
Instrument hardware
problem
The temperature
measurement error with
the PCR/Thermal
module has occurred.
It is not possible to
continue the library
preparation.
Stop the library run
Follow the on-screen instructions to remove
the used reagent cartridge and sample deck
Switch off the instrument
Switch on the instrument to initiate the self-
test/diagnostic sequence.
Reload the MagicPrep NGS and try to
repeat the library preparation.
If the error still exists, please contact Tecan
Customer Support.
6.6
MagicPrep NGS Instrument Functional Test
The protocol below outlines the steps necessary to run a functional library preparation test of the
MagicPrep NGS system.
When setting up the MagicPrep NGS system for the first time, it is useful to perform a functional
test in addition to the hardware test to confirm performance and set a baseline for your system.
A functional test may also be advised after a system error where a hardware failure was not
detected.
Kit
: Revelo DNA-Seq Enz for MagicPrep NGS
Input
: 100 ng human genomic DNA (NA12878, Coriell (https://www.coriell.org), Cat #: GM12878)
Number of PCR cycles
: 6
6.6.1 Starting the run:
1. Follow the instructions provided in the Revelo DNA-Seq Enz for MagicPrep NGS user guide.
6.6.2 Quantify the libraries:
1. Remove the libraries from the MagicPrep NGS instrument.
2. Measure the library yield using a Qubit. Follow the instructions for measuring DNA
concentration on a Qubit.
6.6.3 Specifications:
The total yield should be
≥ 200 ng.
The coefficient of variation (%CV) across the 8 samples should be
≤ 20%.
%𝐶𝑉 =
𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 𝑑𝑒𝑣𝑖𝑎𝑡𝑖𝑜𝑛
𝑚𝑒𝑎𝑛
∗ 100
