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Operating Manual

M01534_v1.1 MagicPrep NGS Instrument Operating Manual

Page 1 of 39

Operating Manual
MagicPrep™ NGS Instrument

Summary of Contents for MagicPrep NGS

Page 1: ...Operating Manual M01534_v1 1 MagicPrep NGS Instrument Operating Manual Page 1 of 39 Operating Manual MagicPrep NGS Instrument...

Page 2: ...Manual Page 2 of 39 Document Change History Title MagicPrep NGS Instrument Operating Manual Part Number M01534 ID n a Translated from n a Version Issue Document History 1 0 2022 04 12 Initial Release...

Page 3: ..._____________________________11 2 6 Safety Elements________________________________________________________11 2 7 Decontamination Declaration______________________________________________12 3 Technica...

Page 4: ...ecifications _________________________________________________________34 7 Support ______________________________________________________________35 8 System Care ______________________________________...

Page 5: ...that allow the unattended and fully automated preparation of next generation sequencing NGS libraries from nucleic acid samples i e RNA or DNA previously extracted and purified from biological sample...

Page 6: ...may be useful when using the MagicPrep NGS instrument The Doc IDs listed below are root numbers Therefore they do not contain information about the language document version or the medium data storag...

Page 7: ...agicPrep NGS Instrument Operating Manual Page 7 of 39 For safety conventions and symbols refer to chapter Safety 2 Illustrations The illustrations may show component versions which are not relevant to...

Page 8: ...rds Signal Word Meaning Signal Word Meaning Indicates a hazardous situation that if not avoided will result in death or serious injury Indicates a hazardous situation that if not avoided could result...

Page 9: ...rbidden Damaged safety devices must be replaced immediately as described in this manual The MagicPrep NGS must not be modified in any way without prior consultation and written approval of Tecan Tecan...

Page 10: ...od and process validation Defining the installation has been completed Ensuring that all people in contact with the MagicPrep NGS are adequately trained Ensuring the availability of appropriate protec...

Page 11: ...xtensive application knowledge Limited system knowledge Command of local languages Command of English Instructing the operator Writing running and validating methods Helping the operator to solve prob...

Page 12: ...ghly decontaminated in the following circumstances In the event of accidents e g spillage etc Before returning the MagicPrep NGS to Tecan e g for repair Prior to storage Prior to disposal In general b...

Page 13: ...ng information Identification data Model REF Ordering information and revision level SN Serial number Date of manufacture YYYMMDD Technical data U f Supply voltage Volts frequency Hertz Manufacturer s...

Page 14: ...3AH250V Frequency 50 60 Hz Overvoltage category II Fuse rating information where T and H are Time lag and High breaking capacity respectively 3 4 Data and Power Connections A MagicPrep NGS power inpu...

Page 15: ...30 C 86 F or below 3 6 Emission and Immunity Noise emission 60 dBA sound pressure measured at a distance of 1 meter from the instrument EMC The MagicPrep NGS instrument has been tested and found to c...

Page 16: ...te tips and several rows of PCR tubes which may contain additional reagents A reagent cartridge specific for the library preparation method is also inserted into the MagicPrep NGS instrument This cont...

Page 17: ...d with a set of single use disposable assemblies containing the generic reagents used in all the MagicPrep NGS system workflows 4 3 Reagent Cartridge The Reagent Cartridge is a single use consumable c...

Page 18: ...llow your institution s safety procedures for working with chemicals and handling of biological samples Follow good laboratory practices and safety requirements Wear a lab coat disposable gloves and p...

Page 19: ...not designed to carry extra weight Two person lift Two people are needed to lift the package and unpack 5 2 Unpacking The MagicPrep NGS instrument has been designed to be operator installable The Magi...

Page 20: ...g protective material from the front and rear of the instrument Using two people one at each end lift the MagicPrep NGS instrument out of the white cardboard tray remove the plastic packaging and plac...

Page 21: ...nal and ready to be used for NGS library preparation Details on preparing the MagicPrep NGS run and loading samples are outlined in the specific reagent kit user guides Internal Condensation The Magic...

Page 22: ...NA Seq Mech for MagicPrep NGS User Guide Doc ID M01537 6 1 Loading the Sample Deck and Reagent Cartridge Load the samples and magnetic beads into row 1 of the PCR tubes in the tube carrier sample deck...

Page 23: ...h or catch on a hand when inserted into the instrument Risk of Injury When opening the Magic Prep NGS front door and inserting or removing the reagent cartridge and or sample deck please take care not...

Page 24: ...within the MagicPrep NGS door opening The door has a cutout area to ease the alignment of the sample deck with the relevant opening Push the sample deck all the way in until it is felt to clip or clic...

Page 25: ...ion run It is possible to stop a MagicPrep NGS library run before it has completed It is not recommended that run be stopped once it has started However it may be necessary to stop a run that is under...

Page 26: ...tmospheric pressure 1013 hPa 10 This is equivalent to starting a run late on a Friday evening night and being retrieved on a Monday morning Follow the on screen instructions More detailed information...

Page 27: ...t there is a new software package to install and will proceed The circle on the touch screen will show the progress of the software installation This process normally takes less than a minute After th...

Page 28: ...the screen on the right will be displayed Use the slider bar on the right of the screen to move up and down through the Event log Press Back to return to the Instrument Log screen 6 5 2 2 Export Log f...

Page 29: ...e is shown on the right Typical errors that may happen are outlined in the table below In most case the problem or error issue will be resolved by pressing Continue and following the on screen instruc...

Page 30: ...tart If the error persists please contact the Tecan Customer Support line for further support Reagent Cartridge Problem A failure to detect the presence of the reagent cartridge has occurred It is not...

Page 31: ...operating on the latest released SW version Switch off the instrument Switch on the instrument to initiate the self test diagnostic sequence If the error still exists please contact Tecan Customer Sup...

Page 32: ...to repeat the library preparation If the error still exists please contact Tecan Customer Support Instrument motor problem An error has been detected with one of the motor systems It is not possible t...

Page 33: ...on the instrument to initiate the self test diagnostic sequence Reload the MagicPrep NGS and try to repeat the library preparation If the error still exists please contact Tecan Customer Support Erro...

Page 34: ...the steps necessary to run a functional library preparation test of the MagicPrep NGS system When setting up the MagicPrep NGS system for the first time it is useful to perform a functional test in ad...

Page 35: ...upport Press Continue to return to the Settings screen For help with any of our products please contact Tecan Genomics Technical Support at 650 590 3674 direct or 888 654 6544 option 2 toll free U S o...

Page 36: ...ter 8 2 Cleaning Agents The MagicPrep NGS instrument surfaces sample deck frame and stand can be cleaned and decontaminated using typical laboratory cleaning agents as listed below and have been caref...

Page 37: ...ontamination Declaration form The MagicPrep NGS instrument weighs 18 8 kg and should be lifted by two people Using two people at each end lift the MagicPrep NGS instrument from the bench and place it...

Page 38: ...to the shipping pallet Place two packaging straps around the ends of the packaging round the underneath of the pallet to secure the assembly together Tighten the straps such that the pallet and packag...

Page 39: ...ectronic equipment separately 9 2 Local Requirements People s Republic of China Marking for the Restriction of the Use of Hazardous Substances in Electronic and Electrical Products The People s Republ...

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