TANNER Scientific Cryostat TN60, Operating Instructions Manual, Page: 27 / 44

27 Manual_TN60_2021-09-001.00_EN_Tanner.docx
8.11 MANUAL CUTTING
Deactivate the hand wheel stop.
Release the finger protection.
Turn hand wheel evenly clockwise.
A counter clockwise movement will also result in cutting and incremental advance of the specimen.
Approach specimen either by trimming or slow approach.
Set the required section thickness. Change entries by
pressing or . The selected section thickness is
indicated in the display.(e.g. 7 µm in this example)
TUE 13:05:05
CUT: 7 µm Trim: 19µm
Cha: -20 °C (-22) QF: 0
Status:OK 0000
Turn hand wheel until some even sections have been obtained.
Clean the microtome knife or disposable blade (always away from the cutting edge)
with a cold brush.
Position anti-roll plate onto the microtome knife or disposable blade and cut. If
necessary readjust the height of the guide plate.
Leave hand wheel with handle at its lowest point (specimen holder underneath the
microtome knife or disposable blade).
Transfer the section onto a slide.
i
Leave brush always in the chamber to keep it cold.