Sutter Instrument Lambda 721 Operation Manual Download

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Novato, CA 94949

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415-883-0572 Email: [email protected]

Lambda 721

Ultra-High-Speed Optical Beam Combined

7-Channel Wavelength Switching

LED Light Source System

With TTL & USB Interface for External Control

Operation Manual

Rev. 1.10c ((20210517)

Summary of Contents for Lambda 721

Page 1: ...0128 Web www sutter com Fax 415 883 0572 Email info sutter com Lambda 721 Ultra High Speed Optical Beam Combined 7 Channel Wavelength Switching LED Light Source System With TTL USB Interface for Exter...

Page 2: ...ii LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 Copyright 2021 Sutter Instrument Company All Rights Reserved LAMBDA 10 and SmartShutter are registered trademarks of Sutter Instrument Company...

Page 3: ......

Page 4: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517...

Page 5: ...s designed for use in a laboratory environment that has low electrical noise and mechanical vibration Surge suppression is recommended at all times Fuse Replacement Replace only with the same type and...

Page 6: ...materials with this instrument is not recommended and if undertaken is done so at the users own risk Do not operate if there is any obvious damage to any part of the instrument Avoiding Physical Injur...

Page 7: ...ION 5 2 1 Unpacking 5 2 2 Getting Started 5 2 3 Configuration 7 DIP Switches 7 3 OPERATIONS 9 3 1 Controls 9 3 1 1 Power Switch 9 3 2 Indicators 9 3 2 1 LED On Off State Indicators 9 3 3 Connectors 9...

Page 8: ...1 Get LED ON OFF Status S or s Command 22 4 3 1 Get Lambda 10 3 Compatible Controller Type and Configuration Command 22 4 3 1 Get Lambda 10 3 Compatible Status Command 23 5 MAINTENANCE AND RECONFIGURA...

Page 9: ...ompt 11 Figure 3 9 TTL Mode start screen 11 Figure 3 10 TTL Mode running screen 12 Figure 3 11 Use Ring Buffer prompt 12 Figure 3 12 Run existing Ring Buffer prompt 12 Figure 3 13 Ring buffer running...

Page 10: ...0 Table 4 10 B or b Command Stop Loading the Ring Buffer entry value 20 Table 4 11 B or b Command Ring Buffer entry values 20 Table 4 12 Change the on off state of one or more LEDs M or m Command 21 T...

Page 11: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 ix Table E 9 M Command 1st argument 2nd byte values for ON OFF state bit encoding for all LEDs and groups 44...

Page 12: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 x This page intentionally blank...

Page 13: ...ng more in depth technical information towards the end of the chapter The next chapter describes how to install the system followed by one or more chapters that provide operation instructions and endi...

Page 14: ...r each position including the reflections that occur Traditionally combining more than two light sources required the use of a dichroic ladder Dichroic mirrors which switch from transmission to reflec...

Page 15: ...elength selection and beam reflection using Semrock STR Filters 1 2 2 Common Applications Fluorescent microscopy Calcium imaging FURA Optogenetics High speed wavelength selection The Lambda 721 is des...

Page 16: ...configurations with fewer than 7 light sources sources should be filled from lowest to highest number of reflections to ensure the greatest light output This filter is installed at 18 angle of incide...

Page 17: ...506 506 Narrow Band 530 561 590 617 630 660 740 850 or 940 Each cube s optics mirror filter are especially installed and aligned according to the LED s wavelength chosen Power Cord 3mm Liquid Light G...

Page 18: ...the Lambda 721 operating the controls on the front panel and making connections to the rear panel WARNING DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE The output of the light or the light guide should b...

Page 19: ...xternal computer control The following figures show the locations of these connectors on the rear panel of the unit followed by the location of the configuration switches and their definitions It is r...

Page 20: ...f Up Disabled On Down 3 Camera Mode while running the Ring Buffer Disabled Stays ON until next strobe Off Up Enabled Turns OFF before next strobe On Down 4 I O interface return of current Ring Buffer...

Page 21: ...r rear panel 3 1 Controls 3 1 1 Power Switch Main power switch turns unit off on 3 2 Indicators 3 2 1 LED On Off State Indicators The LED on off state indicators are located vertically LED 1 through 7...

Page 22: ...ven different LEDs as the output On power up the following two screens are displayed momentarily Figure 3 3 Startup screen Figure 3 4 Product and firmware revision screen The next item displayed is a...

Page 23: ...To exit Lambda 10 Mode press the OFF button Upon exiting Lambda 10 Mode the display is updated with the TTL Mode prompt described next 3 1 TTL Mode The TTL Mode is a mode in which the ON OFF state of...

Page 24: ...e Ring Buffer Mode is a mode in which the ON OFF state of each of the seven LED channels can be controlled by a series of programmable settings that are sequenced by TTL signaling strobing to the RING...

Page 25: ...s displayed Figure 3 14 Edit ring buffer query screen Pressing NO causes the unit to enter Setup Mode Pressing YES displays the Ring Buffer edit screen showing Ring Buffer Entry 1 Figure 3 15 Ring buf...

Page 26: ...TER on LED 7 exits Setup Mode and the Use Ring Buffer prompt is then displayed 3 1 Demo Mode Figure 3 18 Demo Mode prompt screen Pressing YES displays the following screen Pressing NO causes the displ...

Page 27: ...evice driver The first method requires that the VCP device driver be disabled or not installed The second method requires that the VCP be installed and enabled 4 1 1 Virtual COM Port VCP Serial Port S...

Page 28: ...it ordering format of Little Endian Intel most significant byte MSB in the first byte and least significant LSB in the last byte If the platform on which your application is running is Little Endian t...

Page 29: ...13 0D 0000 1101 M CR Completion indicator Tx All 1 0 4 04 00000100 0004 D EOT Turn LED 4 on Rx All 2 0 4 04 00000100 D EOT Command echo 1 13 0D 0000 1101 M CR Completion indicator Tx All 1 0 5 05 0000...

Page 30: ...4 00110100 0052 4 Turn LED 4 on Rx All 2 0 52 34 00110100 0052 4 Command echo 1 13 0D 0000 1101 M CR Completion indicator Tx All 1 0 53 35 00110101 0053 5 Turn LED 5 on Rx All 2 0 53 35 00110101 0053...

Page 31: ...urn values while running the Ring Buffer LED On Off State Tx Delay Rx Ver Total Bytes Byte Offset Len Value ASCII def char Dec Hex Binary LED 1 On Rx All 1 0 49 31 0011 0001 1 LED 2 On Rx All 1 0 50 3...

Page 32: ...mpletion indicator is returned after the Stop Loading Ring Buffer entry see next is sent effectively ending the command sequence 4 3 1 1 B or b Command Stop Loading Ring Buffer Entry Value This ring b...

Page 33: ...61680 F0F0 11110000 11110000 1 240 F0 1111 0000 0240 Rx All 1 1 13 0D 0000 1101 M CR Completion indicator NOTE The B or b command and each 2 byte Ring Buffer entry once transmitted does not return an...

Page 34: ...turns an ASCII digit for every LED that is currently on in 1 7 order Table 4 14 Get LED ON OFF Status S or s Command Tx Delay Rx Ver Total Bytes Byte Offset Len Value Alt key pad Ctrl char ASCII def c...

Page 35: ...87 57 0101 0111 W WB NC Filter Wheel B is not connected 66 42 0100 0010 B 45 2D 0010 1101 78 4E 0100 1110 N 67 43 0100 0011 C 15 5 87 57 0101 0111 W WC NC Filter Wheel C is not connected 67 43 0100 00...

Page 36: ...compatible data block containing filter wheel and shutter status information Rx 1 26 13 0 204 CC 1100 1100 Command echo 1 16 10 0001 0000 Wheel A Speed 1 Pos 0 2 138 8A 1000 1010 Wheel B Speed 0 Pos...

Page 37: ...hat the maximum current in Amps allowed for any new or replacement LED cube be configured in the Lambda 721 The maximum current for any given LED channel must not exceed the Amp rating of the cube ins...

Page 38: ...king if finished setting the current level Figure 5 3 Prompt for finishing setting LED current levels Pressing NO returns to the Set Max Current display Pressing YES displays a message to turn off the...

Page 39: ...id this warranty Warranty work will be performed only at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after whic...

Page 40: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 28 This page intentionally blank...

Page 41: ...optics and filter 385 nm 721CUBE 410 LED cube with optics and filter 410 nm 721CUBE 440 LED cube with optics and filter 440 nm 721CUBE 460 LED cube with optics and filter 460 nm 721CUBE 480 LED cube...

Page 42: ...NUAL REV 1 10C 20210517 30 721CUBE 940 LED cube with optics and filter 940nm NOTE For a full list of available accessories for the Lambda 721 refer to the Products section of Sutter Instrument s web s...

Page 43: ...rd from the power entry module This will reveal a slot just under the edge of the fuse holder Use a screwdriver to pry the holder straight out of the power entry module Figure C 1 Power entry module a...

Page 44: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 32 This page intentionally blank...

Page 45: ...7 in 48 26 x 26 67 x 17 78 cm Weight 23 lbs 10 44 kg Light Guide Core Type Liquid Length 2 m 6 5 ft Diameter 3 mm 0 11 in Electrical Mains voltage 110 240 V 50 60 Hz Power consumption 148 8 W Power co...

Page 46: ...Cable Type Cable Max Length Power entry 3 pin male connector 3 pin power standard female 3 pin male Geographical region dependent Mains power source 10A 250V with safety ground plug 3 meters approx 1...

Page 47: ...N OFF button Stops exits Lambda 10 TTL or Ring Buffer Run Edit modes SETUP Mode Toggles state of current LED Power up Start Lambda 10 Mode YES Using LEDs 1 7 OFF Press ENTER to start TTL Mode running...

Page 48: ...ube placement E 3 External Control Controlling the Lambda 721 externally via computer is accomplished by sending commands over the USB interface between the computer and the relevant connector on the...

Page 49: ...nd command sequence ends with a terminator byte containing an ASCII CR 13 decimal 0D hexadecimal Although most command bytes can be expressed as ASCII displayable printable characters the rest of a co...

Page 50: ...0D 0000 1101 CR Completion indicator Load Ring Buffer B or b Tx All 1 0 66 or 98 42 or 62 0100 0010 or 0110 0010 0066 or 0098 B or b Begin loading the ring buffer 2 200 1 199 Each two byte word value...

Page 51: ...is ON Rx All 2 8 0 0 or 49 55 00 or 31 37 0000 0000 or 0011 0001 0011 0111 NUL or 1 7 Value 0 for all LEDs OFF or ASCII digit 1 7 for each LED that is ON in that order 1 to 7 bytes 1 8 13 0D 0000 110...

Page 52: ...Rx 1 26 13 0 204 CC 1100 1100 Command echo 1 16 10 0001 0000 Wheel A Speed 1 Pos 0 2 138 8A 1000 1010 Wheel B Speed 0 Pos 10 3 2 252 FC 1111 1100 Wheel C prefix byte 10 0A 0000 1010 Wheel C Speed 0 Po...

Page 53: ...ho 1 13 0D 0000 1101 M CR Completion indicator LED 4 On Tx All 1 0 4 04 00000100 0004 D EOT Turn LED 4 on Rx All 2 0 4 04 00000100 D EOT Command echo 1 13 0D 0000 1101 M CR Completion indicator LED 5...

Page 54: ...010 0050 2 Command echo 1 13 0D 0000 1101 M CR Completion indicator LED 3 On 3 Tx All 1 0 51 33 00110011 0051 3 Turn LED 3 on Rx All 2 0 51 33 00110011 0051 3 Command echo 1 13 0D 0000 1101 M CR Compl...

Page 55: ...48 2038 00100000 00111000 1 56 38 0011 1000 0056 8 LED 7 On Tx All 2 0 64 40 0100 0000 0064 16448 4040 01000000 01000000 1 64 40 0100 0000 0064 End of Ring Buffer Tx All 2 0 240 F0 1111 0000 0240 6168...

Page 56: ...On 5 05 00000101 0005 E ENQ ON ON LED 3 2 On 6 06 00000110 0006 F ACK ON ON ON LED 3 2 1 On 7 07 00000111 0007 G BEL ON LED 4 On 8 08 00001000 0008 H BS ON ON LED 4 1 On 9 09 00001001 0009 I HT ON ON...

Page 57: ...On 51 33 00110011 0051 3 ON ON ON LED 6 5 3 On 52 34 00110100 0052 4 ON ON ON ON LED 6 5 3 1 On 53 35 00110101 0053 5 ON ON ON ON LED 6 5 3 2 On 54 36 00110110 0054 6 ON ON ON ON ON LED 6 5 3 2 1 On 5...

Page 58: ...ON ON ON LED 7 6 2 On 98 62 01100010 0098 b ON ON ON ON LED 7 6 1 2 On 99 63 01100011 0099 c ON ON ON LED 7 6 3 On 100 64 01100100 0100 d ON ON ON ON LED 7 6 3 1 On 101 65 01100101 0101 e ON ON ON ON...

Page 59: ...m the controller must be bit byte ordered in Little Endian format This means that the least significant bit byte is last last to send and last to receive Byte order reversal may be required on some pl...

Page 60: ...LAMBDA 721 OPERATION MANUAL REV 1 10C 20210517 48 NOTES...

Page 61: ...r o 19 TTL Mode T or t 18 Turn on a discrete LED or a group of LEDs M or m 21 configuration 36 connectors light beam combiner output 9 Ring Buffer BNC 10 TTL Inputs LED 1 7 BNCs 10 USB device connecto...

Page 62: ...remote interface 15 Replacing and Installing New LED Cubes 25 Ring Buffer BNC connector 10 S safety warnings iii electrical iii safety warnings precautions operational iii safety warnings precautions...

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