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Refrigerated Centrifuge Sigma 3-16KL

Refrigerated Centrifuge Sigma 3-16KL IVD

11 Appendix

Version 03/2014, Rev. 2.6 of 06/11/2015 • sb

Translation of the original operating manual, part no. 07050

Fig. 33: Diagram of linear curves

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Summary of Contents for Sigma 3-16KL

Page 1: ...6KL IVD Refrigerated Centrifuge part no 07050 Sigma 3 16KL Sigma 3 16KL IVD from serial no 146119 Refrigerated Centrifuge Operating Manual Please retain for later use стр 2 д 6 9034 Москва Турчанинов пер Адрес 11 www donaulab ru Тел 7 495 255 33 89 Sigma Донау Лаб Москва Donau Lab Moscow официальный дистрибьютер оборудования ...

Page 2: ...Refrigerated Centrifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD 2 Version 03 2014 Rev 2 6 of 06 11 2015 sb Translation of the original operating manual part no 07050 ...

Page 3: ...on of the original operating manual part no 07050 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522 5007 12 Web www sigma zentrifugen de E mail info sigma zentrifugen de ...

Page 4: ...Refrigerated Centrifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD 4 Version 03 2014 Rev 2 6 of 06 11 2015 sb Translation of the original operating manual part no 07050 ...

Page 5: ...lative centrifugal force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions 17 3 6 Safety instructions 18 3 6 1 Electrical safety 18 3 6 2 Mechanical safety 18 3 6 3 Fire prevention 19 3 6 4 Chemical and biological safety 19 3 6 5 Safety instructio...

Page 6: ...of the rotor 28 6 2 2 2 Installation of angle rotors with a hermetically sealed lid 28 6 2 2 3 Installation of accessories 29 6 2 2 4 Adapters 30 6 2 2 5 Tubes 30 6 3 Control system Spincontrol L 31 6 3 1 User interface 31 6 3 2 Manual mode 32 6 3 2 1 Starting a centrifugation run 32 6 3 2 2 Interrupting a centrifugation run 32 6 3 2 3 Interrupting a deceleration process 32 6 3 2 4 Selection displ...

Page 7: ...0 8 1 4 Rotors buckets and carriers 50 8 1 5 Load bearing bolts 50 8 1 6 Glass breakage 51 8 2 Sterilisation and disinfection of the rotor chamber and accessories 51 8 2 1 Autoclaving 52 8 3 Service 53 8 4 Return of defective parts 54 9 Disposal 56 9 1 Disposal of the centrifuge 56 9 2 Disposal of the packaging 56 10 Technical data 57 10 1 Ambient conditions 58 10 2 Technical documentation 58 11 A...

Page 8: ...Refrigerated Centrifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD Table of contents 8 Version 03 2014 Rev 2 6 of 06 11 2015 sb Translation of the original operating manual part no 07050 ...

Page 9: ...24 55_6 8 d ocx 191 723 2 1 1 2 Intended use The laboratory centrifuge is suitable for the separation of constituents of different densities in mixtures with a maximum density of 1 2 g cm3 The laboratory centrifuge that is marked with IVD is intended for human biological samples including donated blood and tissue in conjunction with diagnostic in vitro applications This means that it is a medical ...

Page 10: ...dm odule 0 10 Allge mein e In for matio nen 010 00 50 N or men und Vorsc hrifte n 26 mod_ 140 531 895 820 2_6 8 docx 1 917 65 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaration of conformity Pos 11 010 Unive rsalm odul e Le erzeil e 0 mo d_1 202 116 244 500 _0 d ocx 114 1 Pos 12 100 Sigma 110 BA Ze ntrifu gen Sigma Proj ekte 3 16KL 010 Allg em eine I nfo...

Page 11: ...er Zen trifu ge 26 mo d_1 4053 189 829 45_ 68 d ocx 191 793 2 1 2 1 Layout of the centrifuge Pos 17 010 Unive rsalm odul e Le erzeil e 0 mo d_1 202 116 244 500 _0 d ocx 114 1 Pos 18 100 Sigma 110 BA Ze ntrifu gen Sigma Proj ekte 3 16KL 020 A ufba u un d Wirk ungsw eise0 20 001 0 0 010 Fu nktions u nd Bedi enel eme nte 3 1 6KL 29 mod _140 532 168 283 5_6 8 doc x 1 973 65 3 1 2 1 1 Functional and op...

Page 12: ... 0 0 020 Typ enschil d 3 16KL 29 mo d_1 405 3216 837 86_ 68 d ocx 197 379 3 1 2 1 2 Name plate 1 Manufacturer and registered office 2 Type 3 Serial number 4 Max speed 5 Max kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for special disposal see chapter 9 Disposal 10 CE mark in compliance with the directive 2006 42 EC 11 Part number 12 Year of manufacture 13 Power consumption ...

Page 13: ...uadratically as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Pos 24 010 Unive rsalm odul e Le erzeil e 0 mo d_1 202 116 244 500 _0 d ocx 114 1 Pos 25 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 20 Auf bau und Wirku ngsweis e02 ...

Page 14: ...gal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an overview of the relationship between speed radius and RCF see chapter 11 2 Speed gravitational field diagram Pos 28 010 Unive rsalm odul e Le erzeil e 0 mo d_1 202 116 244 500 _0 d ocx 114 1 Pos 2...

Page 15: ...0 mod _140 540 400 441 3_6 8 doc x 2 039 49 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name plate see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic wast...

Page 16: ...mbols and notes In this operating manual the following names and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of...

Page 17: ... odul e Le erzeil e 0 mo d_1 202 116 244 500 _0 d ocx 114 1 Pos 40 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 30 Sich erh eit03 0 0 041 Bedie npe rson al IVD 26 mod _14 0531 901 889 1_6 8 do cx 1 920 03 2 1 3 4 Operating personnel Only trained specialised personnel are authorised to operate the unit The persons operating the unit must be familiar with the fundamental health safety an...

Page 18: ... gen Sigma Stan da rdm odule 0 30 Sich erh eit03 0 0 060 00 20 Mec hanisc he Sich erh eit a ußer 1 1 6 1 16K 26 mo d_1 405 319 0243 14_ 68 docx 19 2059 3 1 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifuge observe the following WARNING Do not open the lid when the rotor is in motion Do not reach into the rotor chamber when the rotor is in motion Do not use the centrif...

Page 19: ... Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 30 Sich erh eit03 0 0 060 00 40 C he mische und biolo gische Sicher heit 26 mod _14 053 190 281 41_ 68 d ocx 192 101 3 1 3 6 4 Chemical and biological safety If pathogenic toxic or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines prec...

Page 20: ...ss or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions on the installation of accessories see chapter 6 2 2 3 Installation of accessories WARNING The rotor must be loaded axial symmetrically at equal weights If liquids with a density 1 2 g cm3 are used reduce the ...

Page 21: ...ges such as corrosion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles 10 000 has a service life of 10 000 cycles and a rotor wit...

Page 22: ... Le erzeil e 8p t 1 2 m od_ 133 706 306 2114 _0 docx 65 687 1 Pos 66 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 30 Sich erh eit03 0 0 070 00 40 Sc hutzleit erp rüf ung S pinco ntrol L S E asy Univ ers al 2 7 m od_ 140 5319 043 129 _68 docx 19 224 1 3 1 3 7 4 Earth conductor check For the earth conductor check there is an equipotential bonding screw on the rear panel of the centrifuge...

Page 23: ... 76 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 30 Sich erh eit03 0 0 090 Rest risiken 2 7 mo d_1 405 319 052 885 _68 docx 19 233 9 2 1 3 9 Remaining hazards The unit was built state of the art and according to the accepted safety rules However danger to life and limb of the operator or of third parties or impairments of the unit or other material assets cannot be completely excluded ...

Page 24: ...rbe dingu nge n 27 m od_ 140 531 916 144 2_6 8 docx 1 923 67 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The storage temperature must be above 20 C If you would like to store it for more than one year or if you intend to ship it overseas please contact the manufacturer Pos 83 010 Unive rsalm odul e Le erz...

Page 25: ...gen Sigma Proj ekte 3 16KL 040 Lag eru ng und Tr ansp ort0 40 005 0 Tran spo rtsiche run g 3 16KL Üb ersc hrift 29 mo d_1 405 321 6966 73_ 68 docx 19 7435 2 1 4 5 Transport safety device Pos 89 100 Sigma 110 BA Ze ntrifu gen Sigma Proj ekte 3 16KL 040 Lag eru ng und Tr ansp ort 040 00 51 Tra nspo rtsich eru ng 3 16K L Text 3 6 mo d_1 434 605 719 511 _68 docx 26 390 2 1 The transport safety device ...

Page 26: ...00 _0 d ocx 114 1 Pos 95 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 50 Aufs tellun g u nd Ansc hluss0 50 002 0 En ergie vers org ung 27 mo d_1 405 319 1756 30_ 68 docx 192 409 2 1 5 2 Power supply Pos 96 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 50 Aufs tellun g u nd Ansc hluss 0 50 002 0 0 011 Anschl ussa rt Kalt ger ätes tecke r Sch utzsch alter 32 mod _14 176 0069 1...

Page 27: ...a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S060 00 20 Einschalt en au ße r 6 16HS 27 mo d_1 405 319 2013 40_ 68 docx 19 2451 2 1 6 2 Switching the centrifuge on Press the mains power switch The display then illuminates The centrifuge is ready for operation Pos 105 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 106 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a St...

Page 28: ...ifted and fastened again This ensures a proper connection between the rotor and the motor shaft CAUTION When using rotors for microtiter plate formats Ensure that the plate holders are inserted together with the plates into the buckets NOTE The lid screw serves for the fastening of the lid onto the rotor only not for the fastening of the rotor onto the motor shaft Follow the safety instructions an...

Page 29: ... on Zub ehö r 4 plätzig bis 2 16P bi s 3 18KS 2 7 m od_ 140 531 9205 710 _68 docx 1 9250 7 4 1 6 2 2 3 Installation of accessories Only use inserts that are suitable for the rotor see chapter 11 1 Range of accessories All buckets of the swing out roto need to be installed when spinning Always load the axial symmetrical inserts buckets of the rotors with the same accessories and fill to avoid imbal...

Page 30: ... If all of the compartments of a carrier are not used the buckets must be loaded evenly Loading the edges of a bucket only is not permissible Pos 116 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 117 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S060 00 20 002 0 0 050 Ge fäße au ßer 4 5 L 27 mo d_14 053 192 116 22_ 68 d ...

Page 31: ... dien obe rfläch e L 27 mo d_1 405 3192 507 04_ 68 docx 192 619 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start key 2 Function knob 3 Display 4 Stop key 5 Lid key...

Page 32: ...ned before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will be displayed in the lower right hand corner of the display Pos 126 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 127 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd...

Page 33: ...he parameters speed and RCF can be changed during the centrifugation Fig 11 Setting the speed value or the RCF value Pos 132 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 133 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S Spinco ntr ol L Ze nt6 06 0 0 030 00 20 0060 L aufzei t 2 7 m od_ 140 531 9263 257 _68 docx 1 9274 ...

Page 34: ...n this case the symbol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol Fig 13 The function Runtime as of the set speed is activated Continuous run During the continuous run the runtime of the centrifuge is unlimited and must be stopped manually The centrifuge accelerates...

Page 35: ...10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S Spinco ntr ol L Ze nt6 06 0 0 030 00 20 0070 Tem per atur nur KL 2 7 m od_ 1405 319 266 022 _68 docx 19 277 3 4 1 6 3 2 3 Temperature The set value is displayed in the upper area of the field and the actual temperature in the rotor chamber is displayed in the lower area Temperatures between 10 C and 40 C can be presele...

Page 36: ...ooling process the set temperature can be modified within the range below the actual temperature The Rapid Temp program will be stopped under the following conditions The set value is reached The program will stop with a sound signal if this function is activated The stop button is pressed The program will be stopped prematurely A parameter is changed except for the temperature and rotors buckets ...

Page 37: ...ere are rotors with several different types of possible buckets the various combinations will be displayed one after the other Confirm the input The selected rotor or rotor bucket combination will be adopted Fig 18 Preselection of a rotor or a rotor bucket combination Automatic rotor identification system If the centrifuge is equipped with an automatic rotor identification system the input mode wi...

Page 38: ...s can be found in Pos 140 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 141 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S Spinco ntr ol L Ze nt6 06 0 0 030 00 20 0100 Aut omatis che D eckelö ffnu ng 27 mod _14 053 192 838 31_ 68 d ocx 192 857 4 1 6 3 2 6 Automatic lid opening function AutoOpen The automatic lid opening...

Page 39: ... desired setting The bar remains visible as long as the cursor is placed over the symbol Fig 21 The sound signal Buzzer is activated Pos 144 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 145 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S Spinco ntr ol L Ze nt6 06 0 0 030 00 20 0120 Pro gra mms pe rre 27 mo d_1 405 319 2...

Page 40: ... quit the cycle display Fig 23 Cycle display for rotor 11650 and bucket 13421 Reaching the maximum number of cycles When the maximum number of cycles is reached for a rotor or bucket a corresponding warning signal will be displayed every time that the centrifuge is started the start key lid key and entire display will flash Fig 24 Display when the maximum number of cycles is reached flashing When ...

Page 41: ... padlock symbol flashes The input lock is activated Proceed in the same manner in order to deactivate the permanent input lock Fig 25 Padlock symbol indicating an activated input lock Pos 150 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 151 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 060 B etrie b_Spinc ont rol L S Spinco ntr ol L Ze nt6 06 0 0 03...

Page 42: ...ations are indicated by a flashing display Any storage locations that are already occupied will be overwritten during the saving process Save the program in the desired location The program is now saved NOTE The functions Auto Open Buzzer ProgLock and Cycles cannot be entered as part of a program Fig 26 Saving a program Pos 154 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 1...

Page 43: ...nda rd mod ule 060 B etrie b_Spinc ont rol L S060 00 40 Aussch alten 27 mo d_1 405 319 212 716_ 68 docx 19 259 1 2 1 6 4 Switching the centrifuge off Open the centrifuge when it is not in use so moisture can evaporate Switch the centrifuge off by pressing the mains power switch Pos 158 01 0 Univ ersal mod ule Absc hnittsw echsel 0 mod _12 021 245 140 62_ 0 docx 4 18 1 Pos 159 01 0 Univ ersal mod u...

Page 44: ... cord is not plugged in Plug in power cord correctly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several Power off on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly ...

Page 45: ...n the centrifuge lid Switch off the mains power switch and disconnect the power cord from the socket Remove the plug see figure item 1 from the opening at the side panel e g with a screwdriver Fig 28 Position of the opening for the emergency lid release The plug is connected with a string Pull the string in order to release the lid Then reinsert the stopper into the opening WARNING Do not unlock o...

Page 46: ... will be given 40 45 Temperature error only for refrigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error Allow to slow down Power off Eliminate the imbalance 50 59 Lid error Press lid key Close lid Remove foreign matter from the opening of t...

Page 47: ... the event of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de Outside Germany Contact our agency in your country All agencies are listed at www sigma zentrifugen de Sales Partners NOTE If you would like to utilise our service please state th...

Page 48: ...1 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 172 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 173 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 080 W art ung und Inst andh altun g08 0 0 010 Wa rtun gsar beite n 28 mod _14 053 2061 397 8_6 8 do cx 1948 03 2 1 8 1 Maintenance Pos 174 10 0 Sigm a 10 0 BA Zentrif uge n ...

Page 49: ...nst andh altun g08 0 0 010 00 20 Zu beh ör au ßer 8K 28 mo d_1 4053 206 182 47_ 68 d ocx 194 845 3 1 8 1 3 Accessories CAUTION For the care of the accessories special safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under running water if they have come into contact with any liquids ...

Page 50: ...om high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weaken the material without any visible signs Check the material regularly at least once a month for cracks visible damage of the surface pressure marks signs of corrosion other ...

Page 51: ... the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Remove the grease with the glass and metal particles with a cloth If necessary repeat this procedure Pos 187 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 188 10 0...

Page 52: ...g autoclaving Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118 30 40 Polyamide buckets 115 118 30 40 10 Polycarbonate polyallomer lids for angle rotors 115 118 30 40 20 Polycarbonate tubes 115 11...

Page 53: ...d during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dism...

Page 54: ...loyees and of the environment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging NOTE We will return the unit if no declaration of decontamination ...

Page 55: ...anner Please use the original packaging if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be downloaded online from www sigma zentrifugen de Service Overhaul and repair Pos 195 01 0 Univ ersal mod ule Absc hnittsw echsel 0 mod _12 021 245 140 62_ 0 docx 4 18 1 Pos 196 01 0 Univ ersal mod...

Page 56: ... unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defective parts Comply with any other applicable local rules and regulations Pos 199 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 200 10 0 Sigm a 10 0 BA ...

Page 57: ...Connected load kVA Power consumption kW Max current consumption A Thermal circuit breaker A 1 4 at 220 240 V 50 60 Hz 1 3 at 120 V 60 Hz 1 3 at 100 V 50 60 Hz 1 01 at 220 240 V 50 60 Hz 1 03 at 120 V 60 Hz 1 06 at 100 V 50 60 Hz 5 9 at 220 240 V 50 60 Hz 11 0 at 120 V 60 Hz 13 5 at 100 V 50 60 Hz 10 0 at 220 240 V 50 60 Hz 16 0 at 120 V 60 Hz 16 0 at 100 V 50 60 Hz Performance data Max speed rpm M...

Page 58: ...C to 35 C Max relative humidity of air 80 up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 206 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 207 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 100 Tec hnisch e Dat en1 00 002 0 Tech nische Doku men tatio n 2 8 m od_ 140 532 068 1369 _6...

Page 59: ...buckets no 13180 2 sets rectangular carriers no 18015 and 48 PPCO tubes 15 ml no 15023 Radius max 17 1 cm radius min 7 6 cm 4 500 3 871 11182 Swing out rotor 12 x 50 ml culture tubes complete no 11180 incl 2 sets buckets no 13190 2 sets adapters no 17346 and 12 tubes no 15151 Radius max 17 2 cm radius min 7 5 cm 4 500 3 894 11222 Swing out rotor for microtiter plates incl 1 set carriers no 13222 m...

Page 60: ...2182 Angle rotor 12 x 5 ml Eppendorf reaction caps incl hermetic aluminium lid no 17182 Software version 32 or higher Radius max 8 4 cm radius min 4 3 cm 15 500 22 562 12349 Angle rotor 48 x 1 5 2 0 ml for reaction vials e g no 15008 15040 incl hermetic lid 17349 Radius max 10 0 cm radius min 6 5 cm 14 000 21 913 19776 Angle rotor for 6 x 50 ml culture tubes e g no 15151 incl hermetic aluminium li...

Page 61: ... Adapter for 1 Greiner tube 15 ml Ø 17 22 x 95 100 mm 1 set 2 pcs suitable for 12150 13079 Bottom adapter for 1 tube 50 ml e g no 15051 15052 1 set 2 pcs 13080 Adapter for 1 culture tube 50 ml e g no 15151 1 set 2 pcs suitable for 12155 without lid 12159 13081 Adapter for 1 culture tube 15 ml e g no 15115 1 set 2 pcs suitable for 12155 without lid 12159 13082 Adapter for 1 tube 50 ml max Ø 28 8 x ...

Page 62: ...stated for the tube When using glass tubes the maximum value of 4 000 x g must not be exceeded except special high strength glass tubes please refer to the information provided by the manufacturer Pos 214 01 0 Univ ersal mod ule L eerz eile 0 m od_ 120 211 624 450 0_0 docx 11 4 1 Pos 215 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 110 A nha ng11 0 0 010 00 20 R adien de r Rot ore n...

Page 63: ...ting manual part no 07050 Pos 217 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 110 A nha ng11 0 0 020 Dr ehza hl Schwe ref eld Di agr amm 2 8 m od_ 140 5320 705 642 _68 docx 19 515 3 2 1 11 2 Speed gravitational field diagram Fig 31 Speed gravitational field diagram Pos 218 01 0 Univ ersal mod ule Seit enwec hsel 0 m od_ 120 211 624 4312 _0 docx 10 5 1 ...

Page 64: ...re inverted images of the acceleration curves and are assigned the same numbers An exception is curve 0 It decelerates brakeless spin out In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Linear curves The slope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared...

Page 65: ...original operating manual part no 07050 Fig 33 Diagram of linear curves Pos 220 01 0 Univ ersal mod ule Seit enwec hsel 0 m od_ 120 211 624 4312 _0 docx 10 5 1 Linear Curves 0 100 200 300 400 500 600 700 800 900 1000 0 50 100 150 200 250 Time sec Speed rpm curve 0 curve 1 curve 2 curve 3 curve 4 curve 5 curve 6 curve 7 curve 8 curve 9 98 7 6 5 4 3 2 1 0 ...

Page 66: ...ars 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x 1 16 1 16K 12135 5 years 20x 1 16 1 16K 12137 5 years 20x 1 16 1 16K 12500 7 years 6 15 6K15 6 16 6 16K 12600 7 years 6 16S 6 16KS 13218 20 000 4 16 4 16S 4 16K 4 16KS 6 16 6 16S ...

Page 67: ...O saturated 1 1 4 1 1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1 1 2 1 1 1 1 1 1 3 Ammonium hydroxide NH3 H2O 30 1 3 4 1 1 2 1 1 1 Aniline C6H7N 100 1 3 4 1 2 4 4 ...

Page 68: ... 1 2 1 Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 100 3 4 2 4 1 1 Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 3 1 Dipropylene glycol mono methyl ether C4H10O 100 3 1 4 1 4 4 4 4 1 1 Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 4 1 Ethylene c...

Page 69: ...O 100 1 2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nitric acid HNO3 10 1 4 1 4 1 1 1 1 4 3 Nitric acid HNO3 100 4 4 4 4 4 4 1 4 1 Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 4 1 Oleic acid C18H34O2 100 1 1 1 2 1 1 1 3 1 Oxalic acid C2H2...

Page 70: ...H 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4 6 1 4 1 4 1 1 1 1 2 3 Sulphuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 4 3 Tallow 100 1 1 1 1 1 1 1 1 1 1 Tetrahydrofuran THF C4H8O 100 3 1 4 1 3 4 4 4 1 3 1 Tetrahydronaphthalene C10H12 10...

Page 71: ...4 Rev 2 6 of 06 11 2015 sb 71 Translation of the original operating manual part no 07050 Pos 225 10 0 Sigm a 11 0 BA Zentrif uge n Sigm a Pr ojekt e 3 16K L 11 0 Anha ng1 10 006 0 Konf or mitäts erklä run g 3 16KL 3 16KL IVD 29 mo d_14 053 217 454 57_ 68 d ocx 197 561 2 1 11 6 EC declaration of conformity ...

Page 72: ...Refrigerated Centrifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD 11 Appendix 72 Version 03 2014 Rev 2 6 of 06 11 2015 sb Translation of the original operating manual part no 07050 ...

Page 73: ...Refrigerated Centrifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD 11 Appendix Version 03 2014 Rev 2 6 of 06 11 2015 sb 73 Translation of the original operating manual part no 07050 ...

Page 74: ...trifuge Sigma 3 16KL Refrigerated Centrifuge Sigma 3 16KL IVD 11 Appendix 74 Version 03 2014 Rev 2 6 of 06 11 2015 sb Translation of the original operating manual part no 07050 E nde der Liste für Tex tma rke I nhal t ...

Page 75: ...started 44 Centrifuge decelerates during operation 44 Centrifuge is inclined 44 Centrifuge was moved during run 44 Centrifuge cleaning and care 48 Changes in colour 52 Changes in structure 52 Chemical and biological safety 19 Chemical resistance of plastic 50 Cleaning agents 51 Cleaning the bores of angle rotors 49 Cleaning the centrifuge 48 Closing the lid 18 27 Communication error 46 Condensatio...

Page 76: ...29 30 50 Imbalance error 46 Imbalance monitoring system 22 Importance of the operating manual 9 Important information 16 Improper loading 44 Infectious substances 51 Inflammable substances 19 Informal safety instructions 17 Initial start up 27 Input lock 41 Inspection by the manufacturer 53 Installation of accessories 29 Installation of angle rotors with a hermetically sealed lid 28 Installation o...

Page 77: ...8 Radius 14 Range of accessories 59 Rapid Temp program 36 Reaching the maximum number of cycles 40 Relative centrifugal force RCF 14 33 Removal of the transport safety device 25 Remove glass particles and metal dust from the rotor chamber 51 Responsibility of the operator 17 Return of centrifuges spare parts or accessories 54 Return of defective parts 54 Risk of electrical shock 18 Rotor chamber 3...

Page 78: ...es 46 Table of rotors and accessories with a different service life 21 66 Technical data 57 Technical documentation 58 Temperature error 46 Temperature inside the rotor chamber 22 Temperature monitoring system 22 Temperature range 57 Temperature value not reached 44 Thermal circuit breaker 57 Thermal stress 48 Time range 57 Toxic substances 19 48 Transport safety device 25 Tubes 30 Type 57 Type of...

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Sigma Sigma 3-16KL, Operating Manual

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