ZEBV-5084A
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4. Remarks
4.1 About making your samples for iMScope TRIO
(a) Back and Front of the Slide glass
The slide glass to use in the iMScope TRIO is thing called ITO glass. One side of the ITO-glass is coated
with Indium TinOxide to make it conductive. Prepare a commercial ITO coated glass slide (26 × 76 mm,
1.0-1.2 mm thick, e.g.ALDRICH #578274).
Affix the sample to the conductive side of the glass slide. It is possible to determine which surface of the
glass slide is conductive by measuring resistance with an instrument such as a tester.
(b) The Place to Mount Sections
Avoid the outermost 5 mm of either side and the outermost 1 mm (sectionB) of either end when affixing
the sample. Because of the instrument composition, we can not analyze that part.
(c) Section Thickness
To avoid the section damage and the instrument trouble, we ask you to slice the tissue within 1mm
thickness. In general, you can obtain better results with thinner sections. So, we recommend you to slice
tissues within 10µm thick.
(d) Frozen Section Attachment
When you mount frozen sections on to the ITO-glass, please make sure those sections are firmly stuck to
the glass. If a part of the section is separated from the ITO-glass, you can NOT obtain any signals from
that part. When you mount the frozen sections to the ITO-glass, please follow the method below.
Mount the frozen sections to the ITO-glass→ Touch the back of the ITO-glass with your finger→ Thaw the
section (this step makes the sections to stick firmly to the ITO-glass)→ Refreeze immediately.