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4.4.2. Sample loading
•
Load the samples using a gel loading pipette tip. During sample loading the
pipette tip should be 1 - 2 mm above the bottom of the well to minimise dilution of
the sample and to keep the sample as a tight layer.
•
Avoid cross-contamination by overloading the sample wells.
•
Fill unused wells with the equivalent volume of sample buffer to maintain uniform
electrical resistance across the gel.
4.4.3. Start of electrophoresis
•
Close the safety lid firmly making sure that the electrical connectors form a good
contact.
•
Connect the electrophoresis apparatus to the power pack and connect the power
pack to the mains supply, e. g. SERVA BluePower 500x4 (BP-500x4). Turn all
settings to zero before turning on the mains supply. Adjust the controls to the
desired settings. Follow manufacturers instructions.
4.4.4. End of run
•
Turn the power supply settings to zero, turn off mains supply and disconnect the
power leads.
•
Remove the safety lid, take out the internal gel running unit and discard the buffer.
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DOMINIQUE DUTSCHER SAS
