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4.4. Performing electrophoresis  

 

4.4.1. Insertion of precast gel cassettes into the inner core running unit 
 

 

Clean the silicone gasket, located in the inner core unit. If the gasket becomes 
unseated from its groove simply press it back into place. 

 

 

Insert the precast gel cassette into the inner core running unit. 

 

 

 

 

When running only one gel, a dummy plate is required on the opposite side to 
retain the required buffer level. 
 

 

Place the gel running unit into the buffer tank using the locating pegs as guides. 

 

 

Add the appropriate volumes of running buffer to the inner and outer buffer 
chambers (see Table 1).

 

 

  Important:

 

Do not

 overfill (please see marking “max fill”).

 

 
 
 
 
 
 
 
 
 
 
 

DOMINIQUE DUTSCHER SAS

Summary of Contents for BlueVertical PRIME

Page 1: ...STRUCTION MANUAL BlueVertical PRiME Vertical Electrophoresis Chamber SERVA Electrophoresis GmbH Carl Benz Str 7 D 69115 Heidelberg Phone 49 6221 138400 Fax 49 6221 1384010 e mail info serva de http ww...

Page 2: ...hat your expectations are reflected in the successful use of the apparatus First check that the apparatus has been received complete and undamaged following shipment Any faults or losses must be notif...

Page 3: ...ral care and maintenance 6 4 3 Gel and buffer volumes running conditions 6 4 4 Performing electrophoresis 7 4 4 1 Insertion of pre cast gel cassettes into the inner core running unit 7 4 4 2 Sample lo...

Page 4: ...nstruction All acrylic joints chemically bonded Doubly insulated cables rated safe up to 1000 volt Gold plated electrical connectors corrosion free and rated safe up to 1000 volt Safety lid with integ...

Page 5: ...removing the safety cover Do not attach the safety lid to the internal gel running unit while it is out of the bottom buffer chamber Do not exceed the maximum operating voltage or current see Table 1...

Page 6: ...ble1 The running conditions Table 2 vary according to the number of gels and their composition The current required will increase in proportion to the number of gels providing that the voltage is not...

Page 7: ...he precast gel cassette into the inner core running unit When running only one gel a dummy plate is required on the opposite side to retain the required buffer level Place the gel running unit into th...

Page 8: ...ctrophoresis Close the safety lid firmly making sure that the electrical connectors form a good contact Connect the electrophoresis apparatus to the power pack and connect the power pack to the mains...

Page 9: ...rode connectors with tissue Ensure that the connectors are clean and dry before usage or storage see chapter 4 2 For further information about the SERVA BlueLine please contact the technical service o...

Page 10: ...43277 SERVAGel TG PRiME 8 16 Tris Glycine Gel 8 16 12 43279 10 43280 2D 43281 SERVAGel TG 8 Tris Glycine Gel 8 Available upon request please inquire 12 43208 10 43209 SERVAGel TG 10 Tris Glycine Gel...

Page 11: ...DS Electrophoresis Buffer 20x 42560 Buffers for native PAGE SERVA Tris Glycine Native Sample Buffer 2x 42528 SERVA Tris Glycine Native Electrophoresis Buffer 10x 42530 Native Anode Buffer for BN CN 10...

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