XFp Extracellular Flux Analyzer User Manual
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Suitable Locations for the XFp Analyzer
The XFp Analyzer is designed for laboratory use. The internal environment for the XFp Cell Culture
Miniplate is controlled to a preset temperature; therefore, laboratory room temperature must be
maintained within the range listed in the specification table in Section 2. The miniplate tray
temperature can be monitored using the status display on the upper right portion of the user interface.
The XFp Analyzer uses optical detection technology to measure extremely low levels of fluorescent
emission from analyte sensors. Therefore, although the instrument has been designed to shield room
light, excessive light (such as direct sunlight) should be avoided.
Safety Considerations
WARNING:
The protection provided by this instrument may be compromised if it is used in a manner not
specified by Seahorse Bioscience.
Safe operation of the XFp Analyzer requires that all covers be securely attached and plate tray door is
closed. This also prevents heat loss and system cooling, which can affect data quality.
The door opens automatically when the tray is extended, allowing the operator to insert or remove the
assay consumables. The operator must exercise caution during the loading of the miniplate/cartridge in
order to avoid the possibility of a pinch hazard. After the miniplate/cartridge is placed securely on the
tray, the operator’s hand must be removed from the area of the tray before continuing the assay. After
the command is given to continue the assay via the user interface, the tray will move slowly back into
the instrument and the door will close. An optical sensor is used to determine the status of the door.
The XFp Analyzer has heaters around the miniplate that maintain a stable temperature. Typically, the
temperature will be maintained at 37°C, as monitored by temperature sensors and controllers
embedded in above the tray. A thermal fuse will disable the heater should it reach an abnormally high
temperature.
The user shall not replace the power cord provided with any other power cord that is rated at less than
what is specified in Section 2, dependent on the power mains of the country the instrument will be used
in.
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