XCell™ C410:V3 Controller
User Guide
41 repligen.com UG-C410v3-06
When working with an XCell ATF® Device connected to an unpressurized vessel, refer to
an overview of positioning the filtration assembly, P-Box and E-Box relative to the bioreactor.
Example 1
Using an unpressurized bioreactor with an XCell ATF® 6 Device:
When using a bioreactor that cannot be pressurized such as a glass vessel or a disposable vessel, i.e.,
SUB, the connection between the filtration assembly and the SUB will most likely not be an SIP type
connection shown in
. Placement of the filtration assembly and controller relative to the
bioreactor will, however, not change significantly.
Using a vessel that cannot be pressurized, both pressure and vacuum services are needed.
Repligen offers custom, disposable connections to most commercial SUBs. The connection
procedure between the filtration assembly and vessel are provided separately.
Start
1.
Connect the filtration assembly to bioreactor per separate instructions.
2.
Place the P-Box in proximity of the filtration assembly.
3.
Connect Signal cable between E-Box and P-Box.
4.
Connect E-Box to an appropriate electrical power source
5.
Connect the air line, exhaust line and pump line to their respective ports on the P-Box. Do
not connect the pump line from the XCell™ C410:V3 Controller to the diaphragm pump at
this point.
6.
Power On the E-Box. Wait for system to boot up and display the XCell ATF® Device Log On
screen.
7.
Log On, the main screen will be displayed. Proceed to the Set Up screen.
8.
Enter settings.
Note:
XCell ATF® Device selection - Select XCell ATF® 6 Device. Note reactor pressure and
∆
height-
enter 0 and measured difference between diaphragm pump clamp and vessel liquid level in cm.
1.
Power up compressed air and vacuum services, confirm pressure and vacuum services are
on by observing the respective pressure gauges.
2.
Connect pneumatic pump line to diaphragm pump air filter.
3.
Activate the P2 trend on the Main screen. (Any setting adjustments in the P2 screen are
made from the P2 Trend screen).
4.
Press the Start button on the Main screen.
5.
XCell ATF® Device should begin cycling normally.
6.
Observe for conditions described at the end of the last section.
Note:
The air purge from the system after start of XCell ATF® Device. Note also the small up and
down change in vessel liquid level, indicating XCell ATF® Device cyclic flow.
Changing flow rate:
To change flow rates, simply go to the main screen select
P-Flow
SP
. Enter the new flow
rate on the pup-up menu. Press
Yes
to accept current PV and PRV values or press
Default
to
accept default PV and PRV values. Default is recommended when entering new flow rates.
Follow similar procedures to change
E-Flow
SP.
It is recommended to
STOP
the controller
prior to change of flow rate. Following the change, re-start the controller. It will start with
the diaphragm in the fully exhausted position.
