8
When both M and C buttons are pressed together, one
“
*
” signal appears in front of “Retr”
to indicate that user can now press
“
+
” or “
-
“ to adjust the retraction value.
“
+
” or “
-
“ is to increase or decrease the values for sectioning/trimming, or
retraction (when
“
*
” appears in front of Retr).
Right zone
This zone is for time and temperature setting. The two red LED lights above indicate the working
status of the big compressor (left) and the smaller Peltier compressor (right).
The
left
light is on when the compressor starts to work, and the right light turns on
when
pressing to activate fast freezing.
After the cryostat turns on, it automatically enters the initial mode as below:
→ Current weekday (1-7 indicates Monday to Sunday)
→ Current time (00:00 - 23:59)
→
T0
=Chamber
T2
= Spec. head (actual temp. [set temp.])
→
T1
= Quick freeze shelf temp. (actual temp. [Peltier on/off]
Clock setting
The clock can be set by pressing
“D” to adjust weekday and pressing ”H” and “M” to adjust hour
and minute, respectively.
Temperature setting
Press the temperature button, the third line on the LCD screen will shift between T0
(Chamber temperature) and T2 (Specimen head temperature).
These two buttons are to increase or decrease the temperature value for
cryochamber (T0) or specimen head (T2). See Page 16 for optimal temperature
settings per tissue type.
Fast freezing (invalid under standby mode)
This cryostat has two compressors: one large compressor for cryochamber, and one small
compressor for the storage area and specimen head. The small compressor does not run if the
system is in the standby mode. Fast freezing requires both compressors to run and can be
activated only when the system is under the normal working mode. Once fast freezing is
activated, the small compressor
starts in 3 minutes
to cool the storage area and clamp
.
Day 1
Time 12:00
T0 -21 [-24]
T1 -45 [off]