5
Specified performance is guaranteed only when Rainin
disposable tips are used as recommended. Rainin cannot
accept responsibility for poor performance resulting from
the use of tips by other manufacturers.
Rainin tips are molded from premium-grade virgin poly-
propylene plastic. Samples from each lot of tips are
inspected microscopically to ensure that every lot meets
Rainin’s high standards.
Tip Immersion Depth
The recommended depth for tip insertion into the sample
for each Pipetman model is shown in the table below.
Pipetman Model
Volume Range
Immersion Depth
P-2
0.1 - 2 µL
1 - 2 mm
P-10
0.5 - 10 µL
1 - 2 mm
P-20
2 - 20 µL
2 - 3 mm
P-100
10 - 100 µL
2 - 3 mm
P-200
50 - 200 µL
2 - 4 mm
P-1000
1000 - 1000 µL
2 - 4 mm
P-5000
500 - 5000 µL
6 -10 mm
P-10ML
1 mL - 10 mL
6 -10 mm
Tip immersion depth is important. If exceeded, the vol-
ume measured will be inaccurate, possibly out of specifi-
cation. Tip angle is also important. Hold the pipette verti-
cally, or within 20 degrees of vertical.
Pipetting Guidelines & Precautions
Consistency in all aspects of pipetting procedure will
significantly contribute to optimum reproducibility. Use a:
1. Consistent pickup/dispense rhythm while pipetting.
2. Consistent speed and smoothness when you press
and release the pushbutton.
3. Consistent pushbutton pressure at the first stop.
4. Consistent immersion depth.
5. Minimal angle (< 20° from vertical).
Prevent liquids from being drawn into the Pipetman shaft
by taking the following precautions:
1. Use Rainin aerosol-resistant tips, with an internal filter
which acts as a barrier to aerosols and liquids.
2. Never invert or lay Pipetman down if liquid is in the tip.
3. Pipette slowly, holding Pipetman < 20° from vertical.
4. For Models P-5000 and P-10ML Pipetman, use the
special safety filters supplied.
pipetman rev I 9/13/00 3:12 PM Page 7
