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PIPETMAN

®

continuously adjustable digital pipettes

CONTENTS

Description .....................................................................1
Autoclaving.....................................................................2
Volume Indicator ............................................................2
Safety Filter, P-5000, P-10ML........................................2
Operation........................................................................3
Tip Selection...................................................................4
Tip Immersion Depth ......................................................5
Pipetting Guidelines & Precautions ................................5
Pre-Rinsing Recommended ...........................................6
Reverse Mode Pipetting .................................................6
Pipetting Liquids of Varying Density ..............................6
Temperature Considerations..........................................7
Acids and Corrosives .....................................................7
Pipetman Storage...........................................................7
Troubleshooting and Repairs .........................................8
Sample Splash ...............................................................8
Leaks, Inaccurate Sampling ...........................................8
Removing/Replacing Ejector Arm ..................................9
Service, Calibration and Repair....................................10
Contacting Rainin .........................................................10
Performance Specifications .........................................11
Replacement Parts ........................................Back Cover

FIGURES

Figure 1

Pipetman P-200 .........................................1

Figure 2

Operating Pipetman ...................................4

Figure 3

Removing Tip Ejector Arm .........................9

DIAGRAMS FOR REPLACEMENT PARTS

Models 

P-2, P-10, P-20, P-100 ............................12

Models

P-200, P-1000, P-5000, P-10ML .............13

TABLES

Specifications ...............................................................11

Technical Assistance: 800-543-4030

Call this toll free number for technical consultation and
product information for Pipetman, other Rainin pipettes,
and disposable tips.
Or use e-mail: [email protected]

Pipetman is a US-registered trademark of Rainin Instrument Company,

Inc. Microman is a trademark of Gilson Inc. Pipetman pipettes are manu-

factured under U.S. Patent Nos: 5,413,006 and 5,650,124. Other U.S. and

international patents pending. Copyright 1982-2000, Rainin Instrument

Company, Inc., Woburn, MA 01801, USA.

pipetman rev I  9/13/00  3:12 PM  Page 2

Summary of Contents for Pipetman P-10

Page 1: ...Pipetman continuously adjustable digital microliter pipettes 8 pipettes for volume ranges from 0 1 L to 10 mL P 2 P 10 P 20 P 100 P 200 P 1000 P 5000 P 10ML Pipetman P 200 shown...

Page 2: ...ons 11 Replacement Parts Back Cover FIGURES Figure 1 Pipetman P 200 1 Figure 2 Operating Pipetman 4 Figure 3 Removing Tip Ejector Arm 9 DIAGRAMS FOR REPLACEMENT PARTS Models P 2 P 10 P 20 P 100 12 Mod...

Page 3: ...s in microliters The digital volume indicator simplifies volume setting and virtually eliminates calculation errors All Pipetman models have a highly polished stainless steel piston patented polyethyl...

Page 4: ...are shown below Pipetman Range L Smallest Model Adjustable Recommended Increment L P 2 0 to 2 0 1 to 2 0 002 P 10 0 to 10 0 5 to 10 0 02 P 20 0 to 20 2 to 20 0 02 P 100 0 to 100 10 to 100 0 2 P 200 0...

Page 5: ...e The friction ring prevents unintentional volume changes 3 Attach a new disposable tip to the pipette shaft Press only enough to make a positive airtight seal 4 Press the plunger to the FIRST STOP Th...

Page 6: ...d flexible so that the shaft is not scratched or worn prematurely Tips must be free from microscopic flash and particu lates Flash gives poor precision and accuracy The tip orifice must be the correct...

Page 7: ...is important If exceeded the vol ume measured will be inaccurate possibly out of specifi cation Tip angle is also important Hold the pipette verti cally or within 20 degrees of vertical Pipetting Gui...

Page 8: ...he tip without touching the orifice 5 To dispense rest the end of the tip against the vessel wall and press the plunger to the first stop Hold this position a few seconds or long enough for the liquid...

Page 9: ...contact with corrosive fumes may corrode the piston This will result in premature seal wear and may require refinishing or replacement of the piston Exposure of internal Pipetman components to corrosi...

Page 10: ...05803D which use a grease seal Leaks Inaccurate Sampling 1 Loosened shaft Tighten coupling by hand 2 Split or cracked shaft Remove the tip ejector and inspect the shaft for fracture or split end Repla...

Page 11: ...jector opening over the end of the Pipetman shaft 5 Push the plastic collar into the handle until the tip ejector is securely in place Make sure that tips will fit properly on the shaft Figure 3 Tip E...

Page 12: ...U S only from Rainin It is NOT necessary to recalibrate the pipette after changing the seal or shaft Recalibration of the pipette is only necessary when the piston is replaced and should only be done...

Page 13: ...ipetman are defined in this manual as follows Accuracy is the closeness of a measured volume to the true volume as specified by the volume setting of the pipette Also known as mean error Precision is...

Page 14: ...e diagrams are for dual adjustment Pipetman and cannot be used on older Pipetman models For convenience old style part numbers are also listed on the back page 12 P 2 P 10 P 20 P 100 A B M L K J H G F...

Page 15: ...13 P 200 P 1000 P 5000 P 10ML A B M L K J H G F C D E A B G F C D E A B D N N A B F G D G F...

Page 16: ...00 n a n a n a n a n a n a 6190 164 6190 164 Filters 1000 n a n a n a n a n a n a 6190 165 6190 165 This column is for P 10ML after S N N05806D For P 10ML pre S N N05803D same as listed above except P...

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