R-Biopharm Rida Cycler, User Manual

Page: 59 / 87

RIDA
®
CYCLER
Version 1.0
|
User manual
59
Normalization failure: The cause of the normalization failure depends on the type of
normalization being performed:
•
Dynamic
– an initial linear region is unable to be found;
The exclusion is set to:
Extensive: samples will be excluded if they display no amplification, if normalization fails or
they fail the fluorescence cut off.
If a sample is excluded its Result column entry of the Cycling Analysis Result table will be set
to Excluded.
Window of Linearity: Any sample which is excluded will not be included in the window of
linearity calculation. In addition, samples may also be excluded from the window of linearity
calculation if:
•
The second derivative maxima occur at the extreme of the data range;
•
The logarithm of the normalized fluorescence trace is not defined near the second derivative
maxima.
If a non-excluded sample was excluded from the window of linearity calculation, then it will not
have an efficiency or R2 value calculation and its Result column entry of the Cycling Analysis
Result table will be set to Excluded from WOL.
12.2 Cycling analysis results table
The results table is organized
to display the mean (x ̅) and standard deviation (xσ_(n-1)) of the
Cq values for sample replicates; and the individual sample results, which are organized, just
below the replicate row, into the following columns:
Fig. 52: Cycling analysis results table