R-Biopharm Rida Cycler, User Manual

Page: 21 / 87

RIDA
®
CYCLER
Version 1.0
|
User manual
21
Once the tubes are loaded, properly fit the caps to ensure the tubes are sealed.
Use the provided capping tool to help with fitting caps properly and avoid cross contamination.
Thumb
Index finger
Capping Tool
The tool is designed to clamp
down on a strip of four caps.
With your thumb and index
finger on either side of the
tool press the caps down into
the strip of four tubes until
firmly in place.
Remove the tool by
unclamping the caps.
Fig. 7: Capping tool
The caps can be removed later to access the post PCR reaction for downstream applications
such as gel electrophoresis or DNA sequencing.
Ensure that post PCR amplicons are handled away from a pre-PCR environment
to avoid contamination issues.
Place the reaction tubes into the rotor keeping the tube tab in line with the marker located on
the rotor label.
Load water tubes in unused wells.
No tubes, empty tubes and tubes with different volumes of liquid all have different thermal
loads on the metal rotor. Variations in thermal load around the rotor can cause significant
thermal gradients both at static temperatures and during ramping, resulting in increased
variability in results.
As the oil overlay prevents evaporation, these Water Tubes can be stored and reused for over a
week.
To achieve optimum temperature uniformity, it is very important to load tubes,
pre-filled with water, into the unused wells of the rotor using the same volume as
that of the reaction tubes.
After loading all the tubes, place the tube clamp at the top of the rotor.
The tube clamp will safeguard against any of the tubes or caps coming out of the rotor during a
run.
Once the lid is closed the instrument is ready to be run.
Failure to close the lid will prevent the run from starting. This is to prevent injury to the user
and/or damage to the instrument.
When the run begins, the lid will be locked into place to prevent it being opened.