R-Biopharm Rida Cycler, User Manual

Page: 38 / 87

RIDA
®
CYCLER
Version 1.0
|
User manual
38
You can Edit or Delete assay libraries from the list.
Fig. 32: Personal assay library
The Information, Assay Profile and Analysis Settings can be viewed once the assay is selected.
Remove assays by using the delete button next to the assay name.
The Run Profile is based on the first assay that is selected. Any following assay selected,
which fails to meet the Run Profile temperatures and hold times, temperature control, or
reaction volume will be determined to be non-compatible.
8.2 Assay profile compatibility
If an assay is selected that has a Profile not compatible to the first assay, the software will
bring up the following warning message:
“The selected assay is not compatible with the run. The assay’s profile and current run’s profile
do not match and cannot be automatically adjusted. Please indicate how you wish to proceed:”
Select from the following options:
Modify Assay:
The assay’s profile will be adjusted to match the run.
This may also add melt steps to the run’s profile if required by the
assay.
Add Without
Modification: The selected assay will be added without modification.
The assay will remain incompatible until it or the run’s profile is
modified.
Cancel: Do not add the assay to the run.
Non-compatible assays are reported with a caution symbol.
Specific reasons for the non-compatibility are reported below the symbol. You can also enter
the Profile for the incompatible assay and select more
… to view all of the reasons. In the area,
you have the choice to:
Modify the run to match this profile: The run’s profile will be modified to make it compatible
with the selected assay. This may cause existing assays to become incompatible.
Modify this assay t
o match the run profile: The assay’s profile will be adjusted to match the
run.
Test any modified profiles on a small subset of samples to confirm these
changes are valid, before running experiments with a large number of
samples to avoid major loss of valuable samples and reagents. The
consequences might be suboptimal performance of the qPCR leading to poor
results