Qiagen PowerLyzer 24, User Manual

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PowerLyzer 24 Homogenizer User Manual 09/2018 44
Recommended settings
Sample name
and type
Bead matrix (lysing
matrix) Setting
(speed – number of cycles x time – pause)
Application
Microbial
Bacteria Garnet 0.15 mm 2000 – 1 x 5 min – 0 s DNA isolation
Bacteria Glass 0.1 mm 2000 – 1 x 5 min – 0 s DNA isolation
Animal
Muscle Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Brain Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Liver Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Kidney Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Lung Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Heart Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Spleen Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Plant
Leaves Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Leaves Metal 2.38 mm 2,000 – 1 x 3 min DNA isolation
Seeds Metal 2.38 mm 2,900 – 1 x 3 min DNA isolation
Stems Ceramic 2.8 mm 4,200 – 2 x 45 s – 30 s RNA isolation
Stems Metal 2.38 mm 2,200 – 1 x 3 min DNA isolation
Roots Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Root Metal 2.38 mm 2500 – 1 x 3 min DNA isolation
Flowers Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Soil
Soil Garnet 0.7 mm 2000 – 1 x 5 min DNA isolation
Soil Glass 0.1 mm Clay: 4000 – 1 x 45 s – 0 s
Loose, high humic: 2500 – 1 x 45 s – 0 s DNA isolation