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CONTENTS 

 

Rolera EM-C

2

 User’s Manual  ............................................................................................ i 

INTRODUCTION ............................................................................................................. 1 

Power Requirements  ................................................................................................... 1 
Host Requirements ...................................................................................................... 1 
IEEE 1394 (FireWire) Interface  ................................................................................. 2 
Cables..........................................................................................................................2 
Imaging Software for Your Camera  ........................................................................... 3 

INSTALLATION .............................................................................................................. 4 

Step 1. Install the 1394 PCI-Express card (Windows Users Only)  ............................ 4 
Step 2. Install Your Imaging Software  ....................................................................... 5 
Step 3. Connect the Rolera EM-C

2

.............................................................................. 5 

CAMERA BASICS  ........................................................................................................... 7 

Turning the Camera On and Off  ................................................................................. 7 
Connecting the Rolera EM-C

2

 to your Optics .............................................................7 

Understanding the LED Indicators  ............................................................................. 8 
Capturing Images with the Rolera EM-C

2

................................................................... 8 

ADVANCED CAMERA CONTROLS ............................................................................. 9 

Cooling Capability ...................................................................................................... 9  
Triggering the Rolera EM-C

2

 Externally .................................................................... 9 

Gain and Offset Controls  ........................................................................................... 11 
Easy-EM Mode……………....................................................................................... 11 

CARING FOR YOUR CAMERA  .................................................................................... 12 
TROUBLESHOOTING .................................................................................................... 13 

Resolving Problems with the camera ......................................................................... 13 
Unresolved Problems - Contacting QImaging Support  ............................................. 14 

GLOSSARY ..................................................................................................................... 15 
APPENDICES .................................................................................................................. 17 

APPENDIX A: External Trigger Port  ....................................................................... 17 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

ii

 

Summary of Contents for Rolera EM-C2

Page 1: ......

Page 2: ...aging or other hardware software or service providers and are used herein for identification purposes only Microsoft and Windows are registered trademarks in the U S and other countries of Microsoft C...

Page 3: ...EM C2 5 CAMERA BASICS 7 Turning the Camera On and Off 7 Connecting the Rolera EM C2 to your Optics 7 Understanding the LED Indicators 8 Capturing Images with the Rolera EM C2 8 ADVANCED CAMERA CONTRO...

Page 4: ...high sensitivity performance for live cell confocal microscopy Power Requirements The Rolera EM C2 can be powered through a single IEEE 1394 FireWire 6 pin to 9 pin or 9 pin to 9 pin cable when the c...

Page 5: ...e available FireWire 1394b port at the back of the camera the other end of the cable plugs into your computer s 9 pin FireWire port QImaging recommends that you install the supplied 1394B PCI Express...

Page 6: ...2 operates on both Windows and Mac OS based systems QCapture Suite Software for both systems is available at WWW QIMAGING COM The easy to use QCapture software gives you complete control over the came...

Page 7: ...heck your computer for an existing FireWire port The Rolera EM C2 connects to your Windows PC via a FireWire port If you already have a FireWire port you may not have to install the IEEE 1394 PCI Expr...

Page 8: ...camera must have the external power connected However should the cooling not be required a single 9 pin to 9 pin connection will operate the camera sufficiently In most cases the FireWire cable provid...

Page 9: ...ower adapter needs to be connected to the camera as shown in Figure 5 below Computer Camera 110 240 VAC power supply Figure 5 FireWire and External Power Supply Connection If you have a laptop compute...

Page 10: ...d the WHITE LED will turn on To turn the Rolera EM C2 off press the power switch again This will turn your camera off The WHITE LED will turn off POWER SIWTCH Figure 6 Power Switch on the Rolera EM C2...

Page 11: ...ng the LED Indicators The LED indicators at the back of the camera give you important information about what your camera is doing WHITE The white LED is the power indicator This LED should always be l...

Page 12: ...externally triggered modes of operation the user inputs a pulse on the External Trigger Port that controls the camera s integration in one of two ways depending on the triggering mode Triggering Modes...

Page 13: ...hitecting your system design Has a system design been developed that incorporates the necessary elements such as a Timing Diagram Flow of Events e g a simple state diagram necessary components e g mic...

Page 14: ...errors incurred when the mapping is performed in the digital domain Easy EM Mode The Rolera EM C2 introduces the new Easy EM Mode This mode optimizes the EM Gain setting on the camera to provide the...

Page 15: ...CCD sensor and circuits are sensitive to static discharge Ensure you are using a static strap or are completely grounded at all times to release any static energy before you clean the cooling window U...

Page 16: ...o find the type of coupler that works optimally with the Rolera EM C2 camera s sensor Point the camera at something in the far distance Loosen the set screw of the C mount ring there are three one on...

Page 17: ...ndex php for a list of all frequently asked questions Your issue may be resolved in one of these faqs Visit http www qimaging com support contact php and fill out a support form online with the detail...

Page 18: ...rge Coupled Device abbr CCD Gain The amount of analog signal amplification The gain is factory optimized for the camera s maximum dynamic range Charge Coupled Device The light sensitive silicon chip n...

Page 19: ...aphragm in the lens that opens or closes to set the aperture the amount of light that passes through the lens to the CCD ms millisecond abbr unit of measure for exposure time ns nanosecond abbr unit o...

Page 20: ...nout for the connector is as follows Pin Number Signal Name Signal Source Description 1 5 VDC User Power for Optocoupler trigger 2 Trigger input User Active Low Trigger Input 3 SYNC A output Camera In...

Page 21: ...3 SYNCA output 4 GROUND U12 2 VCC 8 7 5 V R31 5 SYNCB output 6 GROUND R32 357R 3 R33 357R GND C37 6 0 1uF 5 357R TRIGGER HCPL 061A CN8 2 1 4 3 6 5 MINI DIN R34 357R C38 0 1uF U13 8 VCC 7 6 5 GND 2 R3...

Page 22: ...er the next frame SYNC B This signal has two modes of operation which can be selected in software Refer to the QCAM SDK API documentation for further information on setting these parameters Expose Mod...

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