Promega E6150 Operating Manual Download Page 1

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T e c h n i c a l M a n u a l

Quantus™ Fluorometer
Operating Manual

INSTRUCTIONS FOR USE OF PRODUCT E6150.

PRINTED IN USA.
Revised 1/20

Part# TM396

TM396.0120_EIVD_TM.qxd 1/27/2020 2:50 PM Page a

Summary of Contents for E6150

Page 1: ...T e c h n i c a l M a n u a l Quantus Fluorometer Operating Manual INSTRUCTIONS FOR USE OF PRODUCT E6150 PRINTED IN USA Revised 1 20 Part TM396 TM396 0120_EIVD_TM qxd 1 27 2020 2 50 PM Page a...

Page 2: ...shooting 15 A Common Problems 15 B Error Messages 16 6 Appendix 17 A Updating Firmware 17 B Warranty and Service 17 C Certificate of Decontamination 18 D Related Products 19 7 Summary of Change 19 1 I...

Page 3: ...y Includes Quantus Fluorometer USB Power Supply USB Cord Outlet Adapter Kit 4 adapters supplied for international compatibility Quick Reference Guide Operating Manual Product Registration Card Shippin...

Page 4: ...on sensor Excitation Peak Red 625nm Blue 470nm Excitation Filters Red 640nm shortpass wavelengths up to 640nm Blue 495nm shortpass wavelengths up to 495nm Emission Filters Red 660 720nm Blue 510 580nm...

Page 5: ...ques Veuillez contacter votre repr sentant Promega local concernant la destruction de l appareil Veuillez respecter les exigences de votre tablissement concernant la destruction des accessoires 9630TA...

Page 6: ...nt may be impaired Do not disassemble the unit The Quantus Fluorometer accepts 0 5ml PCR tubes Do not force larger tubes into the tube holder Use caution around solvents because they may corrode the p...

Page 7: ...etting Up the Quantus Fluorometer 1 Position the Quantus Fluorometer on a flat surface 2 Select the appropriate outlet adapter for your wall outlet from the Outlet Adapter Kit provided Promega Corpora...

Page 8: ...un setup exe Follow prompts to install the software If NET 4 0 framework is not installed on the computer the software installer will prompt the user to download and install the framework software ava...

Page 9: ...s Fluorometer protocol has calibration values associated with the specific assay reagent Once the protocol of interest is calibrated the Quantus Fluorometer will save the calibration values for future...

Page 10: ...0 single concentration standard curve 1 400 RNA Cat E3310 high concentration standard curve 1 400 RNA Cat E3310 low concentration standard curve 1 2 000 ssDNA Cat E3190 high concentration standard cur...

Page 11: ...electing the desired protocol navigate to the Calibration screen 2 Place the blank sample into the tube holder and close the lid Select Read Blank and the fluorescence in relative fluorescence units R...

Page 12: ...tered is 100 200 0 5 4 Choose the appropriate fluorescence channel Red Excitation 640nm shortpass emission 660 720nm Blue Excitation 495nm shortpass emission 510 580nm 5 Select Save Note To edit or de...

Page 13: ...it is within the linear range of the standard 1 Press any button to wake up the Quantus Fluorometer The Home screen will appear displaying the last protocol used the last concentration measurement an...

Page 14: ...esult history select Clear from the menu list Results can be transferred to a connected computer as the measurements are read using the Quantus Software see Section 2 B The default fields transferred...

Page 15: ...f additional cleaning is needed wipe with a cloth dampened with a mild detergent remove detergent with a cloth dampened with water and wipe dry 4 Do not use organic solvents they may damage the plasti...

Page 16: ...ow concentration then use the low concentration standard and that the standard used is similar to the sample i e if the unknown sample is plasmid DNA then use a plasmid DNA standard Sample concentrati...

Page 17: ...o retain the calibration values Valid on Calibration screen The standard to blank ratio was within the parameters expected by the instrument Press Save to retain the calibration values and proceed to...

Page 18: ...e update only when instructed by Promega Technical Services Follow the instructions located at www promega com quantusfluorometerfirmware 6 B Warranty and Service The Quantus Fluorometer comes with a...

Page 19: ...aminated and can be handled without exposing personnel to health hazards Circle the type of material used in the instrument Chemical Biological Radioactive Briefly describe the decontamination procedu...

Page 20: ...00 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Printed in USA Part TM396 Revised 1 20 Page 19 2013 2020 Promega Corporation All Rights Reserved QuantiFluor is a registered trademark o...

Page 21: ...Promega Corporation Promega Corporation 2800 2800 W Woods Hollow Road oods Hollow Road Madison WI 53711 5399 USA Phone 608 274 4330 TM396 0120_EIVD_TM qxd 1 27 2020 2 50 PM Page b...