Princeton Instruments ProEM+ EMCCD, User Manual

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Chapter 5 Operation 59
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4. Verify that the slit is set to 10 µm. If necessary, adjust the Exposure Time to
maintain optimum (near full-scale) signal intensity.
5. Wait until the camera temperature locks at its default temperature.
6. Make sure that the spectroscopy-mount adapter moves freely at the spectrograph
(you may need to loosen the two set screws that lock the sliding tube in place).
7. Select
Align Spectrometer…
from the Experiment Options menu. Review the
displayed information and then click on the Begin button. Typically, this feature
creates three 1-row high ROIs (one near the top of the array, one in the middle,
and one near the bottom) and begins data acquisition. Data will be continuously
acquired and displayed but will not be stored.
Figure 30. Spectrometer Alignment: Before Rotational Alignment
8. Adjust the rotational alignment. You do this by rotating the camera while
watching a live display of the line. Click on the peak you want to monitor during
the rotational alignment. This positions the large cursor to provide a vertical
reference line across all of the ROIs. Rotate the camera until the selected peak is
aligned horizontally in all of the ROIs.
Alternatively, take an image, display the horizontal and vertical cursor bars, and
compare the vertical bar to the line shape on the screen. Rotate the camera until
the line shape on the screen is parallel with the vertical bar.