Princeton Instruments ProEM+ EMCCD, User Manual

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Chapter 6 Advanced Topics 103
103
LightField High Speed ProEM Add-in
Starting with Version 4.2.2, LightField has a
High Speed ProEM add-in that will, with the
click of a button, change the experiment
settings to minimize readout time and
maximize the number of frames that can be
acquired per second. Keep in mind when using
the add-in that there is tradeoff between
increased spectral readout rate and data quality
(increasing the frames per second tends to
decrease data quality).
Note: If you want to get a better
understanding of the interplay of various
setting changes, the next section in this
chapter steps through manually making the
same changes shown in Figure 65.
When a ProEM+ camera icon is in the
Experiment Devices panel, an Add-ins
panel and a High Speed ProEM expander is
added to the set of panels at the left side of the
screen.
1. After opening the expander, you will see
something like the settings shown in
Figure 65.

If camera is a ProEM+:1600, you will
be able to enter the number of rows to
bin.

If it is a ProEM+:512, 1024, or other
camera with a square array, you can
select
Spectroscopy and enter the
number of rows or select Imaging and
select width and height values. Note
that some of the additional settings are
updated when you change the width
and/or height.
2. Click on the Apply High Speed button to replace the current experiment settings
with the high speed settings. Note that the previous and the new frame rate
information is reported. (If you click on
Restore to Defaults , the experiment
settings will be restored to the defaults for the camera.)
3. On the Experiment Settings panel, enter the Number of Frames (for example, 50).
4. Click on the Acquire button when you are ready to acquire the frame set.
Figure 65. High Speed ProEM settings