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A2. Protocol overview.
BlotCycler is set up to run standard protocol for western blot starting with blocking, washing after blocking, and
then primary antibody incubation, washing after primary antibodies, secondary antibodies incubation and
washing after secondary antibody incubation. Each step can be programmed independently. At the end of all
steps the trays are filled with washing solution and shaking is stopped; the antibody containers are also filled
with washing solution to facilitate cleaning.
Note:
In order to skip blocking and primary antibody incubation set the Primary antibody incubation time to
zero. Protocol will start at second washing step.
A3. Programming BlotCycler (protocol set up).
To start programming the operator needs to initialize the system by turning the system OFF and ON using the
ON/OFF switch on the back of the unit. Please wait 3 sec before turning it on.
To start protocol set up press
.
On the new screen you can select existing protocols, modify existing protocol or set up a new protocol. You can
set up and save up to 20 protocols and there are 4 preset protocols that cannot be changed:
To select a preset protocol touch
you will see a choice of four preset protocols, select one, and
choose on which side(right or left) to run your protocol, a new screen to start run will appear (see below)
To add a new protocol press
and then touch white area under “
Change Protocol Name
”. Using keypad
enter new protocol name.
To delete last protocol press
.
To select or modify existing protocol, highlight protocol by touching it and press
.