Precision Biosystems BlotCycler User Manual Download Page 6

 

 

Test run 

Turn on the instrument using the switch on the back of the unit. The display should light up and you should see 
the first screen with information about different functions: 

 

 
Check that the silicone plug is inserted in the tank between the black pumps. Make sure that the waste tubing is 
going down and is not bent (Remember there is no pump in BlotCycler the solution moves under gravity). 

It is 

important for solution removal that the waste tubing is not bent allowing the solutions to drain properly, 
also the outlet of the tubing should not be immersed in the waste solution. 
 
Left side cleaning: 

Fill water 3-4 cm above minimal level (about 2 L); make sure water is not leaking. 

Press 

 button and then press 

 button, select left side. Now you started the cleaning cycle 

on the left side. Make sure that solution is going into the trays and out of the trays. You may stop the cleaning 
cycle at any time as soon you verified that everything on the left side is working. 
 

Right Side cleaning:  

Repeat the same procedure for the right side, adding more solution if needed.

 

Press 

 button and 

select right side. The cleaning of the right side should start. The solution should be coming out from all three 
tubes in each tray.

 

Now the instrument is ready for use. Read the following instructions to program and start using the BlotCycler. 

 

Summary of Contents for BlotCycler

Page 1: ...1 USER MANUAL BlotCycler automated western blot processor Revision 10 0 2 02 2014 software ver 3 1...

Page 2: ...ifications and Safety 3 Unpacking and Testing 5 Overview 7 Programming 8 Instrument Set up and Operation 11 Appendix 16 Troubleshooting 17 Fuse replacement 18 Packing Instruction 19 Accessory Products...

Page 3: ...rator For technical assistance call write fax or email Call 888 490 4443 x 2 Fax 617 812 2672 Email customersupport precisionbiosystems com Write Customer Support Precision Biosystems 241 Francis Aven...

Page 4: ...arly to 50 from 31 C to 42 C 3 Do not install the equipment near a heating element 4 Do not install the equipment at a place where it may be exposed to corrosive gas 5 Do not install the instrument in...

Page 5: ...and save them in case you need to send instrument back for service Packing List BlotCycler with six trays Waste tubing attached to unit Power cord Tray lids 2 Tank lid 1 Tray plugs 4 Transformer 220 2...

Page 6: ...d in the waste solution Left side cleaning Fill water 3 4 cm above minimal level about 2 L make sure water is not leaking Press button and then press button select left side Now you started the cleani...

Page 7: ...tion vials Pump Antibody container Overview BlotCycler consists of programming display four to six trays for blots trays 1 3 on the left side and trays 4 6 on the right side tank with container for pr...

Page 8: ...r Interface overview User interface contains 4 buttons press this button to select existing or set up a new protocol press this button to start a protocol press this button to check the status of the...

Page 9: ...otCycler protocol set up To start programming the operator needs to initialize the system by turning the system OFF and ON using the ON OFF switch on the back of the unit Please wait 3 sec before turn...

Page 10: ...after blocking PA primary Ab incubation time and SA secondary Ab incubation time Note you can skip washing after blocking by selecting zero but you cannot skip washing after primary and secondary ant...

Page 11: ...place membranes in the trays Add blocking buffers to each tray containing a membrane Close the trays by replacing the covers Note tray cover has a cut that should be on the upper side to ensure trays...

Page 12: ...owing screen Press corresponding start button to start cycling for left Tray 1 3 or right side Tray 4 6 A new screen will appear that show the status of current protocol Red blinking dot indicates the...

Page 13: ...rotocol press button for left or right side Note protocol will start from next step if it was close to the end of the previous step Use to stop shaking while loading blot into trays Protocol on other...

Page 14: ...ough cleaning solution otherwise the pumps can be damaged Go to the home screen and press button a new screen will appear Press and select left or right side on pop up window Note you can start anothe...

Page 15: ...is not in operation Go to home screen and press button and then button When all valves are open the beep sound and the message will appear Now you can turn BlotCycler off Note before opening valves ch...

Page 16: ...Use appropriate transfer times After blotting stain membrane to measure transfer efficiency Protein of interest ran off the gel Use positive control and or molecular weight marker to match gel separat...

Page 17: ...and purified water to prepare solutions Wear clean gloves at all times Use forceps when handling membranes Run cleaning protocol with cleaning buffer increase the concentration of cleaning buffer two...

Page 18: ...fuse compartment located on the power entry block using a small flat blade screwdriver or fingernail to gently open the fuse compartment 3 Pull the fuse holder out of the compartment and inspect the...

Page 19: ...umps into the tank If you cannot find T bar use any appropriate material to secure pump inside tank There are two green Styrofoam covers front and back they are slightly different Back Front Remove tr...

Page 20: ...n the box Place insert flat box over instrument if you cannot find flat box use any soft material to fill the gap between instrument and box top surface Close and tape the box Ship to Precision Biosys...

Page 21: ...idization buffer 500 ml BW1000 Washing buffer 10x 1L BW4000 Washing buffer 10x 4L TR1003RL Pair of Block of mini trays right left TR2003L Replacement Block of midi trays left TR2003R Replacement Block...

Page 22: ...g defects are specifically excluded 1 Defects caused by improper operation and maintenance 2 Repair or modification done by anyone other than Precision Biosystems or their authorized agent 3 Use with...

Page 23: ...information or technical assistance call write fax or email Call 888 490 4443 x 2 Fax 617 812 2672 Email customersupport precisionbiosystems com Write Customer Support Precision Biosystems 241 Francis...

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