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TROUBLESHOOTING GUIDE 

 

If a problem occurs during the course of use, please refer to the tables below                

before contacting your distributor. 

 

 

 

OPTICAL 

Problem 

Cause 

Corrective Measure 

Darkness at the periphery 

Revolving nosepiece not in  

Revolve the nosepiece to 
click-stop position by 

or uneven brightness in the  

click stop position 

swinging the objective 

field of view 

  

correctly into the optical path 

  

  

 

  

  

  

Dirt or dust on the viewfield 

Dirt or dust on the lens -  

Clean the lens 

  

eyepiece, condenser,  

  

  

objective, collector lens or 

  

  

specimen 

  

  

  

  

Poor image quality 

No coverglass attached to  

Attach a 0.17mm coverglass 

  

the slide 

  

  

  

  

  

Coverglass is too thick or thin 

Use a coverglass of the  

  

  

appropriate thickness 
(0.17mm) 

  

  

  

  

Slide may be upside down 

Turn slide over so the cover- 

  

  

glass faces up 

  

  

  

  

Immersion oil is on a dry  

Check the objectives, clean 

  

objective (especially the 40xR) 

if necessary 

  

  

  

  

No immersion oil used with 

Use immersion oil 

  

100xR objective 

  

  

  

  

  

Air bubbles in immersion oil 

Remove bubbles 

  

  

  

  

Condenser aperture is closed 

Open or close properly  

  

or open too much 

  

  

  

  

  

Condenser is positioned too 

Position the condenser at 

  

low 

the upper limit 

  

  

 

IMAGE PROBLEMS 

 

  

  

Image moves while focusing 

Specimen rises from stage 

Secure the specimen in the 

  

surface 

slide holder 

  

  

  

  

Revolving nosepiece is not  

Revolve the nosepiece to the  

  

in the click-stop position 

click-stop position 

  

  

  

Image tinged yellow 

Blue filter not used  

Use daylight blue filter 

 
 
 

Summary of Contents for 3032 series

Page 1: ...an 10595 886 2 8712 0600 886 2 8712 2677 fax info polysciences tw US Headquarters 400 Valley Road Warrington PA 18976 800 523 2575 215 343 6484 800 343 3291 215 343 0214 fax info polysciences com www...

Page 2: ...ry hot Do not touch these parts until they have completely cooled Never attempt to handle a hot halogen bulb 6 All electrical connectors power cord should be inserted into an electrical surge suppress...

Page 3: ...g recessed optical surfaces Avoid using an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning mo...

Page 4: ...r Condenser Eyepieces Trinocular Viewing Head 3032 INVERTED MICROSCOPE SERIES 1 Illumination Bracket Stage Base On Off Switch Variable Light Intensity Adjustment Dial Fine Focus Knob Coarse Focus Knob...

Page 5: ...ct all optical surfaces for dust and debris clean if necessary Please save the hexagonal wrench for future use Retain all stryofoam cartons if reshipment is ever required 2 Figure 2 Lamp House 3 Micro...

Page 6: ...n all fingerprints with a dry soft cloth 2 2 2 Installing the Condenser Illumination Assembly Figure 4 1 Insert the condenser illumination unit 1 into the bracket 2 2 Turn the condenser illumination u...

Page 7: ...lick sound This ensures the objective is centered in the optical light path Inspect the objectives regularly for dirt and oil clean if necessary Cover all unused nosepiece holes with a nosepiece plug...

Page 8: ...Installing the Color Filters Figure 11 Allow the color filter to cool completely before you change them Remove the filter holder and then insert the color filter required Mount the color filter so it...

Page 9: ...cord into an electrical receptacle 5 4 Insert the BNC connector plug into the BNC connector jack Ensure the power cord is connected to a grounded receptacle Use of an electrical surge suppressor recep...

Page 10: ...ure 15 Diopter ring Light path selector lever Light switch Tension adjustment collar Main switch Coarse focus knob Fine focus knob Phase contrast slider Color filter holder Aperture diaphragm adjust l...

Page 11: ...1 3 Adjusting the Tension Adjustment Collar Figure 18 The tension of the coarse focus knob has been factory adjusted Rotate the tension adjustment collar in the direction shown by the arrow Figure 18...

Page 12: ...s your container dish or slide Use the appropriate bracket to hold your Terasaki well plate 3 culture dish 4 flask 5 or slide 5 A larger flask may be placed directly on the stage Slides may also be pl...

Page 13: ...If the specimen is not in focus rotate the diopter ring until a sharp image is obtained The diopter range is 5 4 3 2 Interpupillary Distance Adjustment Figure 22 While observing with both eyes hold t...

Page 14: ...pull the light path selector lever out completely Light Path Selector Lever Illumination Proportions Applications Pushing in the lever completely 100 for Binocular observation Binocular observation Pu...

Page 15: ...ed on the objective To adjust the aperture diaphragm remove the eyepiece then looked into the viewing tube Your field of view will appear as Figure 25 The proportion may be changed by turning the aper...

Page 16: ...hase objectives are 10x and 20x They are mounted on the objective turret as described in 2 2 4 5 1 2 Phase Contrast Annulus Slider Figure 27 The phase annulus ring has been centered The phase annulus...

Page 17: ...with normal usage If adjustments are required 1 Place the specimen on the stage and focus as usual 2 Remove the eyepiece without the diopter adjustment and replace it with the centering telescope 3 En...

Page 18: ...ad and remove the protective cap 2 Remove the protective caps on the both ends of the video adapter 3 Screw the threaded end into the CCD CMOS port and tighten the thumb screw on the adapter 3 3 Insta...

Page 19: ...otective cap 2 Install the adapter into the vertical port and tighten the thumb screw 3 Insert the camera adapter into into the camera port Camera magnification objective magnification x camera lens m...

Page 20: ...m Sensitivity and Graduation of Fine Focus 0 002mm Movement Range from the surface focus of stage plate up 8mm down 3mm Mechanical Stage Size 250mm x 160mm Movement Range 120mm width 78mm length Illum...

Page 21: ...the slide Coverglass is too thick or thin Use a coverglass of the appropriate thickness 0 17mm Slide may be upside down Turn slide over so the cover glass faces up Immersion oil is on a dry Check the...

Page 22: ...e upside down Turn the slide over so the high power objectives cover glass faces up Cover glass is to thick Use a 0 17mm cover glass High power objective contacts Slide upside down Turn the slide over...

Page 23: ...at www polysciences com European Headquarters Handelsstrasse 3 D 69214 Eppelheim Germany 49 6221 765767 49 6221 764620 fax info polysciences de Asia Pacific Headquarters Polysciences Asia Pacific Inc...

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