Polaroid DCM 2 Online Online User'S Manual Download Page 57

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Reducing Grain in Long Exposures

You can reduce image grain in long exposures (longer than 500 milliseconds) by

enabling the DMC 2 multi-frame averaging/dark subtract feature.

To enable or disable multi-frame averaging/dark subtract:

Click

Set Preferences

on the

Tools

menu.

Click the

General

tab.

Click the

Multi-Frame Averaging/Dark Subtract

box to enable or disable it.

If you are enabling the feature, select the number of light frames and dark frames.

Higher light frame and dark frame values usually provide more grain reduction.

Click

Summary of Contents for DCM 2 Online

Page 1: ...Online User Guide DMC 2 ...

Page 2: ... 9 Taking Pictures 12 Typical Picture Taking Sequence 12 Turning the Camera On 13 Starting the DMC 2 Software 14 Selecting a DMC Camera 15 Framing and Focusing the Preview 16 Choosing a Capture Area 17 Choosing Color or Grayscale Preview 19 Rotating the Image 20 ...

Page 3: ...26 Creating a Custom Color Temperature Setting 27 Selecting Image Type 29 Selecting Image Size 30 Image File Sizes 31 Verifying Sharp Focus Digitally 32 Capturing the Image 33 Capturing a Rapid Sequence 40 Printing the Image 42 Enhancing the Image with DMC 2 Software 43 Automatically Adjusting Color Balance 44 ...

Page 4: ...ually Adjusting Brightness 50 Adjusting Gamma 51 Viewing a Histogram 52 Adjusting Sharpness 54 Viewing the Full Image 55 Reducing Grain in Long Exposures 56 Adding a Label 57 Adding a Calibrated Micron Marker 58 Creating a Calibrated Micron Marker 59 Deleting a Calibrated Micron Marker 63 Saving and Reusing Program Settings 64 ...

Page 5: ...e Preferences 77 Output Preferences 79 Camera Preferences 85 Using the Color Stacker Utility 86 Creating a Composite Image 86 Color Stacker Preview Options 88 Camera Maintenance 89 Cleaning the Camera Optics 90 Cleaning the Camera Body 98 Obtaining Software Updates 99 ...

Page 6: ...oftware and Setting Up the Camera 105 Unpacking 106 Installing the DMC 2 Software 107 Attaching the Camera to the Microscope 108 Connecting the Camera to the Computer 110 Connecting the Camera to AC Power and Turning On 115 Taking a Test Picture 117 Camera Limited Warranty 119 Radio and Television Interference 120 ...

Page 7: ...roid Offices and Service Centers 125 Technical Support 132 Solving SCSI Communication Problems 133 Changing Sync Transfer Rate on Some Adaptec SCSI Adapters 138 Installing a SCSI Host Adapter 140 Index 141 ...

Page 8: ...r software You should familiarize yourself with the controls of the Acrobat reader Access Help Reader Online Guide Viewing PDF Documents Navigating Pages for more information Polaroid electronic documents provide additional navigation tools at the top of the screen The icons have the following meanings Click to go to the table of contents ...

Page 9: ...Click to display these help pages Click to open the index ...

Page 10: ...her optical instruments The camera uses a 2 million pixel 11mm CCD charge coupled device to capture 14 bit linear RGB images It transfers these images in seconds to an IBM PC or Power Macintosh com patible computer in TIFF format with 1600x1200 or 800x600 pixels On IBM PC com patible systems an additional image size of 400x300 is available ...

Page 11: ...lly adjust exposure Manually adjust color balance brightness contrast and sharpness Verify focus digitally Add a calibrated scale to the image Add a title or other text to the image Transfer the image to your image editing application or hard drive Capture a rapid sequence of images and select one for transfer to your computer Note The DMC 2 software is compatible with DMC 2 and DMC Ie cameras ...

Page 12: ...uters Compatible with standard 63 1 1 or larger c mount adapters the camera attaches easily to microscopes for most types of transmitted light and reflected light micros copy Multiple DMC 2 cameras can be connected in a SCSI chain to a single computer simul taneously for capturing images from multiple instruments without moving the cam era or reconnecting cables The camera also features a tripod m...

Page 13: ...are 3 Frame and focus the preview 4 Choose a capture area 5 Choose color or grayscale preview 6 Rotate the image 7 Zoom the preview 9 Set sensitivity 10 Select color temperature 11 Select image type 12 Select image size 13 Verify sharp focus digitally 14 Capture the image 15 Enhance the image ...

Page 14: ...g the Camera On 1 Turn off the computer 2 Turn the camera power switch to on 1 3 Verify that the green and yellow LEDs are on indicating AC and termination power are on Power switch on 1 Green LED Yellow LED ...

Page 15: ...the cam era to be used in this session To select a camera see Selecting a DMC Camera Macintosh Windows Double click Polaroid DMC 2 The main DMC 2 window appears 1 Select Programs from the Start menu 2 Point to Polaroid Digital Microscope Camera 2 and select Polaroid DMC 2 The main DMC 2 window appears ...

Page 16: ...session Macintosh Windows 1 Click the Preferences button in the main DMC 2 window 2 Click Choose a DMC 2 in the DMC 2 Preferences box 3 Choose the SCSI address of the camera to be used 4 Click OK to activate the camera 1 Click Set Preferences on the Tools menu 2 Click the Cameras tab 3 Click the SCSI address of the camera to be used 4 Click OK ...

Page 17: ... tions 3 If your microscope requires manually switch the light path to the camera 4 If a viewfinder image is not visible in the DMC 2 software main window click the Preview button Note If clicking Preview does not display an image you may need to adjust expo sure or sensitivity before continuing Exposure adjustment instructions Sensitivity adjustment instructions ...

Page 18: ...exposure to obtain an acceptable image on the Viewfinder Display tab of the main DMC 2 window 2 Click the Region of Interest Selection tab 3 Select the size of the frame to be captured 3 4 or 1 2 frame from the Capture Area list A box defining the selected capture area appears on the image 4 Position the capture area as desired by dragging it ...

Page 19: ...5 Click the Viewfinder Display tab to view only the capture area The values avail able in the Image Size box correspond to the capture area you selected 6 Continue with the picture taking sequence ...

Page 20: ... 2 main window in either color or gray scale A color preview provides a more accurate representation of the final image while a grayscale preview refreshes faster To view a color preview image select Color in the main DMC 2 window To view a grayscale preview image deselect Color ...

Page 21: ...Rotating the Image To correct picture orientation you can rotate the preview image 180 degrees by clicking the rotate button When you rotate the preview image the final image is also rotated ...

Page 22: ...ocusing and closely inspecting detail while framing Enlarging the view does not affect the final image To enlarge the preview image 1 Click the image to display the zoom and focus box 2 Drag the zoom and focus box to the area of interest 3 To zoom in click To zoom out click ...

Page 23: ...have not done so calibrate automatic exposure for the current lighting and specimen conditions See Calibrating Auto Exposure 2 Click the Auto button in the main DMC 2 window 3 Wait a few seconds for the DMC 2 software to optimize the exposure ...

Page 24: ...Exposure 1 Manually set exposure to the optimum position for the current setup See Setting Exposure Manually 2 Click Calibrate Auto Exposure on the Tools menu 3 Click the Set button in the Auto Exposure Calibration box ...

Page 25: ...Setting Exposure Manually Drag the Exposure slider right or left to adjust the camera shutter speed for brighten ing or darkening the image Range 20 milliseconds far left to 8 seconds far right ...

Page 26: ...tting Sensitivity Choose a sensitivity camera gain setting from the Sensitivity list Low ISO 25 equivalent Medium ISO 50 equivalent High ISO 100 equivalent The default setting is Medium ISO 50 equivalent ...

Page 27: ...ting Tungsten 3200K Daylight 5500K These color temperatures are appropriate for many lighting situations but best results are often obtained by creating and using a custom color temperature This cus tom temperature appears as Custom in the Color Temperature list See Creating a Custom Color Temperature ...

Page 28: ...ng based on the actual lighting To create a custom color temperature Macintosh Windows 1 Select Calibrate Custom Color Tem perature from the Color Temp popup menu 2 Obtain a properly exposed and focused image in the DMC 2 preview window 1 Click Calibrate Custom Color Tem perature on the Tools menu 2 Obtain a properly exposed and focused image in the DMC 2 preview window ...

Page 29: ...rate 5 Wait for the Calibrate Color Tempera ture box to close 6 To use the custom setting select Cus tom from the Color Temp popup menu 4 Click Begin 5 Wait for the Calibrate Custom Color Temperature box to close 6 To use the custom setting select Cus tom from the Color Temperature box ...

Page 30: ...ox 48 Bit Color 68 7 billion colors 24 Bit Color 16 7 million colors 16 Bit Grayscale 65 000 gray shades 8 Bit Grayscale 256 gray shades The image type you choose affects the size of the image file See Image File Sizes Note Before saving images as 48 bit color or 16 bit grayscale verify that other appli ...

Page 31: ...able if your selected region of interest includes the entire frame If your region of interest is smaller the available image sizes correspond to the size of the selected region Larger image sizes usually improve image quality but they also increase the image file size and time required to transfer the final image from the camera to your computer See Image File Sizes ...

Page 32: ...ol lowing table File sizes are approximate Image Size Pixels 48 Bit Color 24 Bit Color 16 Bit Grayscale 8 Bit Grayscale 1600 x 1200 11 300 kbyte 5 650 kbyte 3 800 kbyte 1 900 kbyte 800 x 600 2 900 kbyte 1 400 kbyte 1 000 kbyte 500 kbyte 400 x 300 Windows only 700 kbyte 350 kbyte 240 kbyte 120 kbyte ...

Page 33: ...and focus box 2 Drag the zoom and focus box to the area of interest 3 Zoom into the area of interest by clicking 4 Click Focus Tool on the Tools menu The Focus Tool box appears 5 Adjust the microscope focus knob until the two colored indicator bars are farthest to the right The red bar indicates the maximum sharpness achieved so far The other bar indi ...

Page 34: ...he computer You have the following three options for capturing the image Capture the image enhance it with DMC 2 software then save it as a TIFF file Capture and save the image immediately as a TIFF file Capture and transfer the image to a plug in compatible application Macintosh or Capture and transfer to a TWAIN compatible application Windows ...

Page 35: ...n dow for enhancement with DMC 2 software 1 Click Capture Enhance to capture the image transfer it to computer memory and display it in the DMC 2 Enhance window 2 Use the controls in the Enhance window to enhance the image characteristics add a text label or add a calibrated micron marker Instructions for enhancing the image ...

Page 36: ...ansfer the image to a plug in compatible application by clicking Transfer Image available only when running the DMC 2 plug in Save the image to disk by click ing the Save button Transfer the image to a TWAIN compatible application by click ing the Transfer button avail able only when running the DMC 2 TWAIN source within the application ...

Page 37: ...t 1 Click the Capture Save button Note On Macintosh systems this option is available only when running the DMC 2 software When running the DMC 2 plug in within another application click Capture Transfer Then save the image within the other application 2 Choose a folder and filename for the image 3 Click Save ...

Page 38: ...thin the other application It is not an option if you are running the DMC 2 program To capture the image and display it in the application click the Capture Transfer button and wait for the image to appear in your application After the image appears you can edit print or save it according to instructions pro vided with the application ...

Page 39: ...pened the DMC 2 program from the Windows Start menu or you opened the DMC TWAIN software by selecting Acquire from an application s File menu If you opened the DMC 2 program from the Windows Start menu 1 In the DMC 2 main window click Set Preferences on the Tools menu 2 Click the General tab 3 Click the Launch External Application box to enable it 4 In the External Application Path box type the pa...

Page 40: ...are running the TWAIN version of DMC 2 software If you are in the DMC 2 main window click Capture Transfer Wait for the image to appear in your program If you are in the DMC 2 Enhance window or the Full Image window click the transfer button Wait for the image to appear in your program After the image appears in your program you can edit print or save it according to ...

Page 41: ...tured images for transfer to the DMC 2 Enhance window or for saving immediately To capture a rapid sequence 1 Set up the camera and obtain a properly exposed image in the DMC 2 main window 2 Connect the remote trigger cable to the camera 3 Click the Rapid Fire button to display the Thumbnail Gallery 4 Press the remote trigger button to capture an image and display its thumbnail in ...

Page 42: ... images when the buffer is full or to begin overwriting the first images captured 6 Right click a thumbnail you want to transfer A shortcut menu appears 7 Click Image Output Size and select an image size 8 To display the image in the Enhance window for image enhancement before sav ing right click the thumbnail again and click Enhance Then adjust color bright ness gamma etc as usual and save the im...

Page 43: ...zed to fill the page Note To print at different resolutions or sizes save the image and open it with an image editing application such as Adobe Photoshop Macintosh Windows Select Print from the File menu Click the print button to open your printer dialog box Choose the appropriate printer settings and click OK ...

Page 44: ...image to another application you can apply the following enhancements Automatically adjust color balance Adjust color balance by selecting a point that should be white Adjust color balance by selecting a point that should be neutral gray Adjust color balance by selecting a point that should be black Manually adjust brightness Adjust gamma View a histogram Adjust sharpness Reduce grain in long expo...

Page 45: ... Color Balance To have the DMC 2 program analyze the current image and automatically set the color sliders to their optimum position click the automatic balance but ton This feature is not recommended for fluorescent imaging ...

Page 46: ...ing a whitepoint in the picture click the white eyedropper button Then click on an area of the picture that should be pure white The area you clicked becomes white and all other colors in the image are adjusted accordingly You may need to repeat the procedure to achieve an optimum white balance ...

Page 47: ...ral gray point in the pic ture click the gray eyedropper button Then click on an area of the picture that should be neutral gray such as a metallic object concrete gray brick or a shadow falling on a white object The area you clicked becomes neutral gray and all other colors in the image are adjusted accordingly ...

Page 48: ...st overall color balance by identifying a blackpoint in the picture click the black eyedropper button Then click on an area of the picture that should be pure black The area you clicked becomes black and all other colors in the image are adjusted accordingly ...

Page 49: ...Manually Adjusting Color Balance Drag the three Color sliders left or right to adjust color balance The effect of your settings appears in the preview image to the left ...

Page 50: ...Manually Adjusting Dark Levels Drag the Dark Levels slider left or right to decrease or increase dark levels The effect of your settings appears in the preview image to the left ...

Page 51: ...Manually Adjusting Brightness Drag the Brightness slider right or left to increase or decrease brightness The effect of your setting appears in the preview image to the left ...

Page 52: ...ting Gamma Drag the Gamma slider right or left to increase or decrease gamma the apparent con trast between the highlights and the shadows The effect of your setting appears in the preview image to the left ...

Page 53: ...ghtness values in the image 0 255 and the vertical scale represents the number of pixels at each brightness value The Channel list lets you select a channel Luminosity Red Green Blue RGB for the histogram The histogram changes to reflect adjustments in the Exposure setting in the DMC 2 main window and other controls in the Enhance window How to view a histogram ...

Page 54: ...the Tools menu If the Enhance window is visible click the histogram button 2 Select a channel for display from the Channel list Luminosity Red Green Blue RGB 3 If necessary adjust the Exposure slider in the main DMC 2 window or adjust the controls in the Enhance window while viewing their effect on the histogram ...

Page 55: ...Adjusting Sharpness Drag the Sharpness slider left or right to decrease or increase sharpness The effect of your setting appears in the preview image to the left ...

Page 56: ... zoom and pan to assure you have captured the desired area and detail and that enhancements you applied have the expected results To view the full image 1 In the main DMC 2 window click Capture Enhance The captured image appears in the Enhance window 2 Enhance the image as needed 3 Click the full image button to display the image in the Full Image window ...

Page 57: ...e To enable or disable multi frame averaging dark subtract 1 Click Set Preferences on the Tools menu 2 Click the General tab 3 Click the Multi Frame Averaging Dark Subtract box to enable or disable it 4 If you are enabling the feature select the number of light frames and dark frames Higher light frame and dark frame values usually provide more grain reduction 5 Click OK ...

Page 58: ... or printed Macintosh Windows 1 Click the Add Text Label box 2 Type a label or other descriptive infor mation in the text box 3 Select a font and a font size 4 If necessary click the Bold box to change the font to bold 1 Click the white Label strip below the image 2 Type a label or other descriptive infor mation ...

Page 59: ...e tool for determining dimensions of features in the image To add a micron marker to the image 1 If you have not already done so create a calibrated micron marker for the micro scope objective you are using Creating a calibrated micron marker 2 Click the Add Micron Marker box 3 Choose the marker appropriate for the microscope objective from the box that ...

Page 60: ...meter horizontally under the microscope objective and focus the microscope to obtain a sharp image of the scale in the DMC 2 main window 2 Click the Add Micron Marker box 3 Click the popup menu next to Add 1 Select Calibrate Objectives from the Tools menu The Calibrate Objectives Tips box opens 2 Place a micrometer horizontally under the microscope objective and focus the microscope to obtain a ...

Page 61: ... from one micrometer scale mark to another The measured distance appears as a green line To correct the distance draw a new line 3 In the Calibrate Objectives Tips box click the Snap button The Calibrate Objective window opens and displays the micrometer image 4 Define a measured distance in the image by clicking on one micrometer mark then clicking on a second micrometer mark An x appears at each...

Page 62: ...lick Save Marker End of Macintosh procedure To remove the second mark click the Clear button To remove the second and the first marks click the Clear button twice 6 Type the distance between the two marks in the Distance box and choose the units of measure for the distance from the Measurement Unit box 7 Type a description for this objective 30 characters max in the objective ...

Page 63: ...ated Micron Marker continued Macintosh Windows no further steps for Macintosh systems 8 If you want this calibrated marker to be the default marker in the Enhance window click the Use as default objective box 9 Click OK ...

Page 64: ...low the Add micro marker box 1 Click Add micron marker at the bottom of the Enhance window 2 Click Delete Micron Marker from the list of micron markers 3 In the Micron Marker box click the name of the micron marker you want to delete 4 Click Delete 5 To remove additional micron markers repeat steps 3 and 4 6 Click Close ...

Page 65: ... you can save them to a file then reload them when necessary If necessary see Program settings you can save To save the current program settings in the current settings file Macintosh Windows 1 In the Enhancement window click Settings 2 Select Save Settings In the Enhancement window click Save Settings on the File menu ...

Page 66: ...name for the new settings file 3 Select Save 1 If the main DMC 2 window is open click Settings on the File menu to open the Settings window Then click New to dis play the Add New Settings box If the Enhance window is open click Save Settings As on the File menu to display the Add New Settings box 2 In the Settings Name box type a descriptive name for the new settings ...

Page 67: ...you saved earlier Macintosh Windows 1 In the Enhancement window click Settings 2 Select the name of the settings to be applied 1 In the main DMC 2 window click Settings on the File menu to display the Settings window 2 Click the name of the settings you want to load 3 Click OK ...

Page 68: ... Windows 1 In the Enhancement window click Settings 2 Select the name of the settings to be applied 3 Click Delete 1 In the main DMC 2 window click Settings on the File menu to display the Settings window 2 Click the name of the settings you want to delete 3 Click Delete 4 Click OK ...

Page 69: ...gram settings for reuse see Saving and Reusing Program Settings the following settings are saved Main DMC Window Settings Enhance Window Settings Color checkbox state Exposure Sensitivity Color Temperature Image Type Image Size Red Green Brightness Gamma ...

Page 70: ...ckbox and the num ber of dark and light frames are not saved On each image capture Cast found on color balance Show preview on startup Use flash Save Viewfinder ROI settings on exit ing the application Overwrite images during Rapid Fire Number of Light Frames Number of Dark Frames Launch External Application External Application Path ...

Page 71: ...emote Capture Transfer to Enhance Window Transfer to Application Save Directly to File Remote Capture Files Folder Remote Capture Files Base Name Remote Capture Files Image Type Highlight property Specular or Non Specular Color Channel Mixture Color Output Scale Grayscale Channel Mixture Grayscale Output Scale ...

Page 72: ...nces dialog box Windows let you choose these categories of settings for the DMC 2 software To display the Preferences box for viewing or changing preferences General Remote Output Cameras Macintosh Windows Click the Preferences button in the main DMC 2 window Click Set Preferences on the Tools menu ...

Page 73: ...tory defaults after you capture a picture Use existing settings Leaves all controls in the Enhance window unchanged after you capture a picture Do auto color balance Automatically analyzes the image and sets the Dark Levels and Brightness sliders in the Enhance window to their optimum values each time you click the Capture Enhance button or the Capture Transfer button in the Main window Also sets ...

Page 74: ...each image capture Remove it Removes any overall color cast tint from the image when per forming auto color balance Keep it Retains any overall color cast in the image when performing auto color balance Always ask If an overall color cast is detected during auto color balance dis plays a dialog box for removing or keeping it ...

Page 75: ...startup Expands the Main window to show a preview image when the DMC 2 program starts Use flash Activates the x sync flash cable connector on the DMC camera when the image is captured The default setting is off disabled X sync flash cable connector ...

Page 76: ... During Rapid Fire When camera buffer becomes full during a rapid fire sequence begins overwriting the first file in the buffer instead of stopping rapid fire Multi frame Averaging Dark Subtract Enables multi frame averaging and dark frame subtract for reducing grain in long exposures longer than 500 milliseconds After enabling this feature select the number of light frames and dark frames Higher ...

Page 77: ... Preferences Continued Launch External Application Starts the image editing application specified in the External Application Path box when an image is saved to disk Displays the saved image in the application ...

Page 78: ...e Remote Capture Captures images using to remote trigger Select if you want to capture multiple images remotely Transfer to Enhance Window Captures images and automatically transfers them to the Enhance window where you can manually adjust color balance dark levels or brightness gamma or sharpness Transfer to Application Captures images and automatically transfers the image to an image editing pro...

Page 79: ...them directly to a file for viewing and enhancing at a later date Remote Capture Files Change Allows you to specify a location for your image files if you select Save Directly to a File Also allows you to select the file format TIFF BMP or JPEG and the base name of the saved files ...

Page 80: ...cular Usually provides best image results with reflected light microscopy specimens containing specular highlights reflective or metallic areas or points much brighter than most of the image Non specular Usually provides best results for reflected light microscopy without specular highlights and for all transmitted light microscopy ...

Page 81: ...put scale for your specimen and the way the image will be used Color Channel Mixture CRT color Optimizes the output image color for display on a monitor Identity Leaves the output image color as detected by the camera Portrait Optimizes the output image color for studio portrait photogra phy software programs ...

Page 82: ...ras or MATRIXIEUSER MTX for DMC Ie cameras Color Output Scale Tone Scale Optimizes the output image tone values for display on a monitor Linear Sets the tone value of each output pixel in proportion to the actual transmittance or reflectance measured by the camera Logarithmic Sets the tone value of each output pixel in proportion to the ...

Page 83: ...Mixture uses the following RGB channels to produce a grayscale image Luminance Digitally provides the effect of a luminance filter for gray scale imaging Red Provides the effect of a red filter Green Provides the effect of a green filter Blue Provides the effect of a blue filter ...

Page 84: ...Grayscale Channel Mixture continued Cyan DMC Ie cameras only Provides the effect of a cyan filter Magenta DMC Ie cameras only Provides the effect of a magenta filter Yellow DMC Ie cameras only Provides the effect of a yellow filter ...

Page 85: ... for display on a monitor Linear Sets the tone value of each output pixel in proportion to the actual transmittance or reflectance measured by the camera Logarithmic Sets the tone value of each output pixel in proportion to the logarithm of the actual transmittance or reflectance measured by the camera ...

Page 86: ...to select the active DMC camera when multiple DMC cam eras are connected to the computer Cameras are identified by their Device IDs SCSI addresses and their Adapter IDs To select a camera click the appropriate line in the window and then click OK ...

Page 87: ... process the fluorochromes in red green and blue channels and view the result in a single composite image Creating a Composite Image Macintosh Windows 1 Use the DMC 2 camera and software to capture and save an image for each of the three color channels You must save the images in the TIFF format 1 Use the DMC 2 camera and software to capture and save an image for each of the three color channels Y...

Page 88: ... appropriate TIFF file 4 Click Create Composite select a folder and file name and save the composite image 2 Open the Color Stacker utility click the Windows Start button point to Polaroid Digital Microscope Camera 2 on the Programs menu and click Polaroid Color Stacker 3 Select an image for each color channel by clicking each color button Red Green and Blue and selecting the appropriate TIFF file...

Page 89: ...review of the composite image as you select files for each color channel By clicking the Options button at the top of the Color Stacker window you can select from one of these composite preview options Show Original Image Show Relevant Channel Add Channel Progressively ...

Page 90: ...Camera Maintenance DMC 2 camera maintenance consists of cleaning the camera when necessary Click on either topic below for more information Cleaning the camera optics Cleaning the camera body ...

Page 91: ...ehind the shutter and the CCD sensor by placing the cap on the camera c mount opening whenever the camera is removed from the micro scope This will also minimize the need to clean the camera optics Caution The DMC 2 camera is a precision optical instrument Carefully follow the instructions in this section when you clean it Failure to do so could damage the shut ter or the anti aliasing filter and ...

Page 92: ...pe optics by comparing two images as follows 1 Take a picture with the camera in its normal position 2 Rotate the camera 30 or 40 degrees and take a second picture of the same speci men If the spot moves in relation to other elements in the picture it is caused by dust on the camera anti aliasing filter or the c mount adapter lens In this case skip to step 3 to determine the location If the dust r...

Page 93: ...t adapter lens a Separate the camera from the c mount b Install the lens cap on the DMC 2 camera c Clean the c mount adapter lens according to the manufacturer s instructions d Reattach the c mount and the DMC 2 camera to the microscope and take a pic ture e If the spot remains in the image follow the camera optics cleaning steps ...

Page 94: ...ter as follows This software opens the shutter allowing you to clean the cam era optics Macintosh Windows a Copy the file DMC 2 SHUTTER OPENER INSTALLER from the DMC 2 CD provided with the camera to the desktop b Double click DMC 2 Shutter No special installation is necessary The shutter opening software is installed during DMC 2 software installation ...

Page 95: ... camera with a lens remove the lens 4 If the camera is not connected to the computer turn off the camera and the com puter Then connect the camera to the computer Clean camel hair brush small Can of clean room grade com pressed air Bulb type manual blower Lint free disposable cleaning wipe such as e wipe or a high grade lens tissue non abrasive and lint free Distilled water or methanol ...

Page 96: ...the computer and turn the camera power off The shutter remains open Macintosh Windows a Double click DMC 2 Shutter Opener b Click Open DMC 2 Shutter c Click OK a Select Programs from the Windows Start menu b Point to Polaroid Digital Micro scope Camera 2 and select Shutter Cleaning Tool ...

Page 97: ...ades and filter are easily damaged If using canned air be sure to hold the can upright to avoid spraying liquid propellant into the camera Also avoid spraying canned air directly at the shutter 9 If the filter is clean skip to step 10 If dust or dirt remains after you use the air and the brush follow these steps a Moisten a lint free wipe with distilled water or methanol b Lightly wipe across the ...

Page 98: ...dust or dirt remains after the first attempt to wipe it away repeat the proce dure with a new clean wipe 10 After the filter is clean close the shutter by turning the camera power switch on and then off 11 Reattach the camera to the microscope ...

Page 99: ...aning the Camera Body If the DMC 2 camera body requires cleaning use a clean soft cloth dampened with water Do not use cleaners of any kind and do not spray water or other liquids directly onto the camera ...

Page 100: ...Obtaining Software Updates For the latest information about DMC 2 software updates visit the Polaroid Web site www polaroid com ...

Page 101: ...0x1200 pixels High resolution 800x600 Normal resolution 400x300 pixels Windows version only Image formats 48 bit color 68 7 billion colors 24 bit color 16 million colors 16 bit grayscale 65 000 gray shades 8 bit grayscale 256 gray shades Image transfer speed Greater than 2 MB second SCSI data transfer ...

Page 102: ... Note Specifications subject to change without notice Dimensions 2 1x7 2x5 1 in 5 3x18 3x13 0cm Power supply 100 250V 0 38A 50 60Hz surge protected input 6 VDC 2 0A output Safety approvals SA CE DVE Limited warranty 1 year parts and labor ...

Page 103: ...re to preview capture and enhance pictures requires a minimum level of computer hardware and software If your system does not meet these requirements contact your Polaroid dealer or your computer dealer PC system requirements Macintosh system requirements ...

Page 104: ...r a TWAIN compliant image processing program such as Adobe Photoshop or Image Pro Microsoft Windows 95 98 Me 2000 or NT 4 0 or later operating system ASPI compliant SCSI adapter and the appropriate Windows drivers 24 bit color display adapter Light microscope with a 63x 1 1 or larger C mount adapter ...

Page 105: ...B of RAM 96MB of RAM for a Photoshop Plug In compliant image pro cessing program such as Adobe Photoshop or Image Pro Apple system software version 8 6 or later QuickTime software version 4 0 3 or later 80MB of available hard disk space 24 bit color display adapter Light microscope with a 63x 1 1 or larger C mount adapter ...

Page 106: ...oftware and Setting Up the Camera 1 Unpack 2 Install the DMC 2 software 3 Attach the camera to the microscope 4 Connect the camera to the computer 5 Connect the camera to AC power and turn on 6 Take a test picture ...

Page 107: ...llustration for some locations 3 SCSI host adapter installation kit for PCs optional con tact your dealer 4 SCSI cable adapter HD50 to DB25 5 SCSI cable 2 meter 6 Polaroid DMC 2 Software CD with TWAIN driver and Polaroid DMC 2 software for Windows 95 98 Me 2000 and NT Adobe Photoshop plug in and Polaroid DMC 2 software for Macintosh systems online user s guide 1 2 ...

Page 108: ...lications that may be running 3 Insert the Polaroid DMC 2 software CD into the drive 4 Start the installation program Macintosh Windows Double click the Polaroid DMC 2 Installer Wait for the DMC 2 install program to start If it does not select Run from the Windows Start menu Click Browse ...

Page 109: ... need a 0 63 or 1 1 c mount adapter not provided compatible with your micro scope Do not use a 0 5 c mount adapter Refer to your microscope manual for more information about c mounts Caution Verify that the threaded end of the c mount adapter does not exceed 0 25 in 6 3mm Longer adapters can damage the camera ...

Page 110: ...croscope 1 Screw the c mount adapter into the camera Note Finger tighten the adapter Do not use tools 2 Insert the c mount adapter into the microscope The camera indicator LEDs should face the front of the microscope Indicator LEDs ...

Page 111: ... host adapter in your computer If your computer does not have an adapter you must install one see Installing a SCSI Host Adapter If your computer is a PC compatible with a SCSI adapter but the adapter is connected to a hard drive installing a second adapter is recommended ...

Page 112: ... addresses of any other SCSI devices Macintosh Windows 1 Run Apple System Profiles usually on the Apple menu If it is not it may be available from www apple com swupdates 2 Click Devices and Volumes to view the SCSI device addresses Look at the SCSI address switch on each SCSI device connected to your system If necessary see the instructions provided with the devices ...

Page 113: ...mera SCSI Address Continued Turn the camera power switch to off 0 and set the SCSI address switch to a value not used by any other device SCSI address switch Do not use 7 8 or 9 not valid addresses Power switch off 0 ...

Page 114: ... and other SCSI devices Use the shortest possible cables Note Turn the computer camera and all other SCSI devices off before connecting or disconnecting any cables Do not connect the camera to the parallel connector on your computer Doing so could damage the camera or computer DMC camera termination on 1 Computer ...

Page 115: ...r computer or the last device in a SCSI chain set camera termination to on 1 Otherwise set termination to off 0 Termination instructions for your other SCSI devices are provided with the devices Note Do not use an external passive terminator to terminate the camera If another device is at the end of the SCSI chain that device must be terminated with an active terminator for proper camera operation...

Page 116: ...C Power and Turning On 1 Connect the camera to AC power and turn the power switch on 2 Verify that the green and yellow LEDs are on indicating AC and termination power are on 1 To AC power Power switch on 1 Green LED Yellow LED ...

Page 117: ...3 Turn the computer on 4 Wait for the system to install the camera driver and display the normal desktop ...

Page 118: ...am 2 Place a specimen under the microscope and focus it through the microscope eye piece Macintosh Windows Double click Polaroid DMC 2 1 Select Programs from the Start menu 2 Point to Polaroid Digital Micro scope Camera 2 and select Polaroid DMC 2 ...

Page 119: ... Image Type box for the test 6 Click Capture Save to capture and save the image to disk 7 Open the saved image with an image analysis application and verify that it was captured satisfactorily Note The color balance of this test image may not be satisfactory because of vari ations in light source color temperatures You can correct this problem by creating ...

Page 120: ...it with the exception of software prove to be defective at any time during the warranty period Polaroid Corporation will at its discretion either replace or repair this item without charge This warranty does not cover damage caused by accident incorrect installation unauthorized modification and misuse or abuse A charge will be made for repair of such damage This warranty excludes all consequentia...

Page 121: ...ipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful inter ference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful inter ference to radio or television reception which can be determined by turning the eq...

Page 122: ...dio TV technician for help Note If your camera SCSI cable has a ferrite core on one end that end must be con nected to the camera FCC Caution To assure continued compliance any changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Product The Polaroid DMC 2 Microscope Camera ...

Page 123: ...and 2 this device must accept any interference received including interference that may cause undesired operation EMI Notice Canada This equipment is a Class A digital apparatus which complies with the Radio Interfer ence Regulations C108 8 M1983 Cet équipment est un appareil numérique de la classe A qui se conforme aux Règle ments sur la brouillage radioélectrique C108 8 M1983 ...

Page 124: ...terference in which case the user may be required to take adequate mea sures Polaroid Corporation certifies that this product conforms to the following standards Low Voltage Directive 73 23 EEC EN60950 IEC 950 EMC directive 89 336 EEC EN 55022 EN 50082 1 IEC 1000 4 2 EN 61000 4 2 IEC 1000 4 3 EN 61000 4 3 ...

Page 125: ...demark Notices Copyright 2001 Polaroid Corporation Cambridge MA 02139 USA Polaroid is a registered trademark Polaroid Corporation All other product names may be trademarks of their respective owners All rights reserved ...

Page 126: ...npark Road Calle Orense 16 2a planta P O Box 163 28020 Madrid North Ryde NSW 2113 Tno 913778103 Tel 02 950 7000 Fax 02 887 2209 Belgique België France Polaroid Belgium N V S A Polaroid France S A Rue Colonel Bourg 111 B P 7 Kolonel Bourgstraat 111 78996 Elancourt Cédex 1140 Bruxelles 1140 Brussel Tel 0141913810 ...

Page 127: ...r 32 F Windsor House Cerqueira Cesar 311 Gloucester Road São Paulo Capital Causeway Bay 01310 921 Tel 852 2894 0333 Tel 55 11 285 6411 Fax 55 11 283 2625 Fax 55 11 288 6521 Fax 55 11 287 5393 Canada Ireland Polaroid Canada Inc Tel 014026897 250 Carlingview Drive Etobicoke Ontario M9W 5G6 ...

Page 128: ...rkerød 21051 Arcisate Varese Tlf 80011887 Tel 0269633253 Deutschland Japan Polaroid GmbH Nippon Polaroid Sprendlinger Landstrasse 109 Kabushiki Kaisha 63069 Offenbach Mori Building No 30 Tel 06995096056 2 2 Toranomon 3 chome Minato ku Tokyo 105 Tel 81 3 3438 8811 Fax 81 3 3433 3537 ...

Page 129: ... P O Box 1219 3600 BE Maarssen Tel 0204874534 New Zealand México Polaroid New Zealand Ltd Polaroid de México S A de C V Camera Services Paseo de la Reforma 195 Piso 17 24 26 Anzac Avenue C P 06500 México D F Auckland Tel 703 1111 Tel 09 377 3773 Fax 566 0505 Del interior al 91 800 70 727 ...

Page 130: ...1473 Skårer Hardturmstrasse 133 Postboks 80 1471 Skårer 8037 Zürich Tlf 80011887 Tel 018385329 Deutsch Tel 018385348 Français Tel 018385349 Italiano Österreich Suomi Polaroid Ges m b H Polaroid Oy Eitnergasse 5A Sinikalliontie 10 1231 Wein 02630 Esposo Tel 06607433 Puh 0800117008 ...

Page 131: ...tória 71 A 4 B 127 27 Skärholmen 1050 Lisboa Tel 084451231 Tel 0505446017 Puerto Rico U K Polaroid Caribbean Corporation Polaroid U K Ltd Centro de Seguros Polaroid Export Operations Ave Ponce de León 701 Wheathampstead House Miramar Santurce 00907 Codicote Road Tel 809 725 6240 Wheathampstead Fax 809 725 5462 Hertfordshire AL4 8SF ...

Page 132: ...U S A Polaroid Corporation Technical Support Toll free 1 800 432 5355 ...

Page 133: ...nd Service Centers Polaroid offers a variety of customer support services Call for details and applicable fees You may also write to Digital Imaging Technical Support 201 Burlington Road 1 2A Bedford MA 01730 Internet Support Technical support is also available over the Internet Connect to the Polaroid Web site at www polaroid com Application Support ...

Page 134: ...Solving SCSI Communication Problems SCSI device not recognized Horizontal lines sections missing from image System stops responding during image transfer ...

Page 135: ... on Turn the power switch on See instructions for turning the device on Cables loose or disconnected Check all cable connections See instructions for connecting the SCSI cables Device turned on after computer Make sure the device is turned on then restart the computer Incorrect SCSI address Make sure the device has a unique SCSI address See ...

Page 136: ... instructions provided with the adapter For Windows NT miniport drivers have the extension SYS Also make sure your system has the latest ASPI layer files in the WINDOWS SYSTEMS folder Updated drivers are often available from your adapter manufacturer s site on the World Wide Web DOS drivers in CONFIG SYS Windows systems only Edit the CONFIG SYS file to disable any SCSI drivers Add REM to beginning...

Page 137: ...proper SCSI chain termination See instructions for setting termination Low quality cables Use only high quality SCSI cables Cables are too long Use shorter SCSI cables Standard SCSI chains are limited to a total length of 6 meters Fast SCSI chains are limited to 3 meters Faulty device termination power fuse Return device for service see technical support ...

Page 138: ...nsfer rate or other settings for Adaptec 2940 and 3940 host adapters only Change the maximum sync transfer rate to its lowest set ting 5 0 or 10 0 depending on the adapter model See Changing Sync Transfer Rate on Some Adaptec SCSI Adapters Device on same SCSI bus with hard drive or CD ROM drive Install a second SCSI host adapter for connecting the device See Installing a SCSI Host Adapter ...

Page 139: ...ync transfer rate or other values are incorrectly set To change the minimum sync transfer rate and other important values for these SCSI adapters 1 Restart your computer and wait for the following message to appear Press Crtl A for SCSISelect TM Utility 2 Immediately press the Control key and A to display the SCSISelect menu 3 Select Configure View Host Adapter Settings ...

Page 140: ...e not recognized Leave all other settings unchanged 5 Press Esc until you are prompted to save the changes 6 Select Yes then press the space bar to restart the computer Setting Optimum Value Initiate Sync Negotiation No Maximum Sync Transfer Rate Minimum 5 0 or 10 0 Enable Disconnection No Initiate Wide Negotiation No ...

Page 141: ...er turn the computer off and follow instructions pro vided by the adapter manufacturer The procedure usually includes software installa tion If your PC uses the Windows NT operating system log in as an administrator before installation Note A SCSI host adapter may have been provided as an option with your hardware device If necessary obtain an adapter from your computer dealer Adapters are not ava...

Page 142: ...ra to 115 area of interest choosing 17 attaching camera to microscope 108 B blackpoint adjusting color balance by selecting 47 brightness adjusting manually 50 C camera body cleaning 98 camera optics cleaning 90 camera preferences 85 ...

Page 143: ... image 33 Cast found on auto color balance 73 color balance adjusting automatically 44 color balance adjusting by selecting gray point 46 color balance adjusting by selecting whitepoint 45 color balance adjusting manually 48 color balance adusting by selecting blackpoint 47 Color Channel Mixture 80 Color Output Scale 81 ...

Page 144: ...blems SCSI solving 133 copyright notice 124 D dark levels adjusting manually 49 dark subtract using to reduce grain 56 device not recognized solving 134 DMC Ie cameras software compatibility 10 Do auto color balance 72 E EMI notice Canada 122 EMI notice Europe 123 ...

Page 145: ...ng manually 24 F FCC notice 120 features DMC 2 camera 9 file sizes based on type and image sizes 31 flash using 74 fluorochromes combining with Color Stacker utility 86 focus verifying digitally 32 focusing and framing preview 16 frame smaller choosing for capture 17 framing and focusing preview 16 ...

Page 146: ...ain reducing in long exposures 56 gray point selecting to adjust color balance 46 Grayscale Channel Mixture 82 Grayscale Output Scale 84 grayscale preview choosing 19 H Highlight Property 79 histogram viewing 52 horizontal lines solving 136 ...

Page 147: ...ge type selecting 29 installing software and setting up camera 105 interference radio and TV 120 Internet obtaining technical support via 132 ISO value setting 25 L label adding to image 57 Launch External Application 76 launching DMC 2 software 14 lines horizontal solving 136 ...

Page 148: ...ge 58 micron marker calibrated adding 58 micron marker calibrated creating 59 micron marker deleting 63 missing image sections solving 136 multi frame averaging using to reduce grain 56 Multi frame Averaging Dark Subtract 75 N navigating in this document 7 Non specular 79 ...

Page 149: ...ts 80 output preferences 79 Overwrite Images During Rapid Fire 75 P package contents 106 picture taking general steps 12 picture taking sequence typical 12 power switch camera turning on 13 preferences general 72 preferences setting 71 preview choosing color or grayscale 19 ...

Page 150: ...es capturing 40 Remote Capture Files 78 remote preferences 77 Reset to defaults 72 responding system stops solving 137 rotating the image 20 S Save Directly to a File 78 Save Viewfinder ROI settings 75 scale calibrated adding to image 58 SCSI address camera setting 111 ...

Page 151: ...a 15 sensitivity setting 25 sequence of images capturing 40 sequence picture taking 12 service centers and offices Polaroid 125 settings program saved in settings file 68 settings program saving and reusing 64 sharpness adjusting 54 Show preview on startup 74 size in pixels image selecting 30 software updates obtaining 99 ...

Page 152: ...are 14 sync transfer rate changing on SCSI adapters 138 system requirements 102 T technical support obtaining 132 termination SCSI setting 114 text label adding to image 57 trademark notice 124 Transfer to Application 77 Transfer to Enhance Window 77 ...

Page 153: ... software 99 Use existing settings 72 Use flash 74 Use Remote Capture 77 W warranty camera limited 119 whitepoint selecting to adjust color balance 45 World Wide Web obtaining technical support via 132 Z zooming the preview 21 ...

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