Chapter 3. Operating Features
11
Application Examples
Example 1
"I have a CoolSNAP
ES
and want to operate the camera in the most sensitive
setting for taking high-resolution, single images."
For this application, the camera should be operated in "alternate normal"
mode to provide the best quantum efficiency and the camera gain should be
set to 2. These settings will operate the camera in its most sensitive mode.
Example 2
"I would like to acquire sequences of images with a CoolSNAP
ES
to study
time-correlated phenomena. My light level is fairly high and I want to
optimize the acquisition rate of the camera."
First, the camera speed should be set to 20 MHz. In addition, the camera
should be put into "normal" mode to take advantage of the overlapping of the
readout with the integration time. Finally, the "clearing" mode of the camera
should be set to "clear pre-sequence" to remove the clearing overhead
between frames. Of course, reducing the region of interest and increasing
binning will always increase the frame rate further.
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