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17 

 

   PRECAUTIONS FOR CULTURES 

 

 Leave space between culture containers. 

Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate 
spacing may result in uneven temperature distribution and CO

2

 gas density. 

 

 Do not place harmful materials in the chamber. 

Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause 
damage resulting from discoloration or corrosion. 

 

 Close the inner door. 

Always close the inner door before closing the outer door. Failure to close the inner door will adversely 
affect performance even if the outer door is closed. 

 

 Open and close the doors gently. 

Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture 
medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the 
glass to confirm that the UV lamp is OFF. 

 

 Be careful when closing the outer door. 

Use the handle when closing the outer door. Holding the door in other places may cause injury by getting 
fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door 
coming loose or the incubator falling over, or it may cause current leakage or electric shock. 

 

 Be careful of the inside of the outer door. 

The inside of the outer door may become hot. 

 

 Avoid using excessive force on the inner door. 

Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result 
in injury from breaking the glass.

 

 

 Check the cause of any alarm buzzer. 

If an alarm buzzer sounds while the incubator is in use, immediately check the cause of the alarm. For 
details on what may cause an alarm buzzer to sound, refer to page 33. 

 

 Thermal conductivity CO

2

 sensor 

It is not abnormal that the thermal conductivity CO

sensor displays CO

2

 density higher than the actual 

density when the chamber humidity temporarily goes down. And take care of the level of humidifying 
water because the lack of water affects the CO

2

 density. 

 

Summary of Contents for MCO-18ACL

Page 1: ...arefully before using this product and keep the operating instructions for future use See page 43 for model number Operating Instructions CO2 Incubator This product is for research use only Not for cl...

Page 2: ......

Page 3: ...NG PAN 22 WATER LEVEL SENSOR MCO 18ACL with MCO 18UVS3 24 ROUTINE MAINTENANCE 25 CORRECT OPERATION 25 SETTING CHAMBER TEMPERATURE AND CO2 DENSITY 26 SETTING OF ALARM RESUME TIME 27 Operation after pow...

Page 4: ...Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect Contact our sales representative or agent if any point in the operating inst...

Page 5: ...lure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property Symbol shows This symbol means caution This symbol means an action is prohibited This s...

Page 6: ...vec mercure For more information on safe handling procedures the measures to be taken in case of accidental breakage and safe disposal options visit ec gc ca mercure mercury Dispose of or recycle in a...

Page 7: ...t is not grounded it will be necessary to install a ground by qualified engineers Never ground the unit through a gas pipe water main telephone line or lightning rod Such grounding may cause electric...

Page 8: ...may cause electric shock or fire When removing the power supply plug from the power supply outlet grip the power supply plug not the cord Pulling the cord may result in electric shock or fire by shor...

Page 9: ...d or alkali in this unit if the container cannot be sealed These may cause corrosion of inner components or electric parts Check the setting when starting up of operation after power failure or turnin...

Page 10: ...ce This symbol indicates an earth This symbol means ON for a power switch This symbol means OFF for a power switch ENVIRONMENTAL CONDITIONS This equipment is designed to be safe at least under the fol...

Page 11: ...3 4 5 6 7 9 inside 10 11 13 19 20 inside 14 15 Rear Right Side When an optional UV system set MCO 18UVS3 is installed 12 16 21 Rear Left Side When an optional UV system set MCO 18UVS3 is installed 18...

Page 12: ...le If MCO 21GC is not used only A is available Refer to page 15 for gas cylinder connection Ensure that the gas pressure is set at 0 03 MPa G 0 3 kgf cm2 G 4 4 psi G Refer to page 42 for gas auto chan...

Page 13: ...ts when the chamber temperature reaches the high limit temperature 8 CO2 inject lamp INJECT This lamp lights when CO2 gas is being injected 9 Digital CO2 density indicator CO2 Normally this indicator...

Page 14: ...setting mode causes the numerical value to shift In key lock mode pressing this key makes key lock ON or OFF See page 28 for details 17 Digit shift key Pressing this key in the setting mode causes th...

Page 15: ...he floor is uneven or the installation is not level the incubator will be unstable and this may cause accident or injury To avoid vibration and noise always make sure that the installation is stable A...

Page 16: ...ith qualified service personnel WARNING To prevent electric shock always ground the incubator If grounding is not possible then have a ground installed by qualified service personnel If the incubator...

Page 17: ...operate and there may an explosive sound When the MCO 21GC is not mounted Using the gas tube that is provided connect the gas regulator for the CO2 gas cylinder to connecting port A for CO2 gas pipe o...

Page 18: ...water spills from the humidifying pan or if the doors are left open for a long time condensation may form on the inside of the doors If that occurs wipe off the condensation with a dry sterile gauze I...

Page 19: ...en closing the outer door Use the handle when closing the outer door Holding the door in other places may cause injury by getting fingers caught in the door Do not lean on the outer door Doing so may...

Page 20: ...on corrosion or rusting Be careful to keep the diluted detergent or water out of the temperature sensor the CO2 gas injection hole and the fan motor shaft bearing Fig 2 Also do not wash the temperatur...

Page 21: ...orrosion or rusting 1 Turn OFF the power switch of the incubator 2 Open the outer and inner doors and pull out all the trays Fig 1 3 Lift the front of the tray supports and pull them out Fig 2 4 When...

Page 22: ...the clamp for the side support Fig 8 10 Remove the clamp Fig 9 11 Lift the side supports off of the pins Fig 10 12 Remove the silicon caps of the access port one each from interior Fig 11 and exterio...

Page 23: ...failure or insufficient decontamination 3 To install the duct confirm 4 pins are securely installed in the 4 holes of duct Fig 2 CAUTION If the duct is fixed insecurely the intended velocity performan...

Page 24: ...low temperature water will lower the temperature and humidity in the chamber CAUTION Set the humidifying pan lengthwise at the center of chamber width At that time push the humidifying pan all the way...

Page 25: ...e to the sensor lead wire Note The humidity in the incubator chamber is adjusted to the optimum setting To prevent condensation on the surface inside chamber and the inner door there is a low temperat...

Page 26: ...ying pan The water level alarm lamp RH PAN blinks if the water level sensor does not come down completely If necessary set the humidifying pan again in the proper location The water level sensor detec...

Page 27: ...15 2 Remove the packing materials from the chamber and inner attachments Clean and disinfect the chamber and all the inner attachments referring to above ROUTINE MAINTENANCE 3 Add approximately 1 5 L...

Page 28: ...figure to 33 0 When pressed the changeable digit is shifted When pressed the figure of settable digit changes 4 Press enter key ENT Set temperature is memorized Left digit in the digital CO2 density i...

Page 29: ...digit changes 6 Press enter key ENT The setting is memorized and the current chamber temperature is displayed The settable alarm resume time are 0 10 20 30 40 50 or 60 minutes The setting is 000 010 0...

Page 30: ...etting change from key lock OFF to key lock ON Description of operation Key operated Indication after operation 1 The current chamber temperature and CO2 density are displayed 2 Press digit shift key...

Page 31: ...door is closed The default setting is for 5 minutes The period can be changed when necessary as shown in the page 31 If the outer door is not opened for at least 12 consecutive hours the UV lamp will...

Page 32: ...ble blue light can be confirmed from the front of the humidifying pan cover UV light is harmful to eyes so do not light the UV lamp when the inner door or the humidifying pan cover is open WARNING Nev...

Page 33: ...zed and the display return to normal display mode The UV timer can be set from 0 minute to 30 minutes The default setting is for 5 minutes When the UV timer is set to zero the UV lamp will not light I...

Page 34: ...ing numerical value shift key set the figure to 001 When pressed the figure of settable digit changes 5 Press enter key ENT Set value is memorized and the display returns to normal display mode This p...

Page 35: ...e temperature on the digital temperature indicator Intermittent tone Remote alarm If the ambient temperature sensor is short circuited E10 is displayed alternately with the temperature on the digital...

Page 36: ...bration key CAL 2 Press the calibration key CAL for about 5 seconds 3 The 3 on the digital temperature indicator blinks and the digital CO2 density indicator goes out 4 Adjust the set value to the act...

Page 37: ...digital CO2 density indicator blinks and the temperature indicator goes out 9 Set the present correct CO2 density with the digit shift key and numerical value shift key then press the enter key ENT 10...

Page 38: ...a week The primary pressure of less than 3 8 MPa G 38 kgf cm2 G 551 psi G means a little gas in the cylinder Replace the cylinder soon It is used near an electric product which emits an electromagneti...

Page 39: ...table enough approx 1 hour after turning ON the power switch or recovering from power failure Note If the problem still has not been solved after trying the above checks and countermeasures or for any...

Page 40: ...pen the valve of both gas cylinders 4 The CO2 gas supply line indicator of CO2 gas supply line currently used is ON Only when MCO 21GC is installed CO2 gas supply line indicator A CO2 gas supply line...

Page 41: ...cylinder before disconnecting it Following shows the procedure to change the CO2 gas supply line manually Only when MCO 21GC is installed Example Change of CO2 gas cylinder from B to A Operation Key...

Page 42: ...the bottom and attach the protective stickers to the 4 corners on the top surface of the incubator that is to be on the bottom Fig A 4 Secure stacking plates A with the screws that are provided 5 Remo...

Page 43: ...41 Fig A Front panel Stacking plate A Protective sticker Hook Stacking plate B Front side...

Page 44: ...ital display Air circulation Fan assisted Air filter 0 3 m Efficiency 99 97 or more Alarms Automatic set temperature alarm Automatic set CO2 density alarm High limit temperature alarm CO2 gas various...

Page 45: ...o C CO2 5 no load CO2 control range 0 to 20 CO2 variation 0 15 ambient temperature 25 o C setting 37 o C CO2 5 no load Chamber humidity 95 R H 5 R H Heat emission Max 1116 kJ h Applicable environment...

Page 46: ...n of the unit Unit interior No contamination Decontaminated Contaminated Others 3 Instructions for safe repair maintenance disposal of the unit a The unit is safe to work on b There is some danger see...

Page 47: ......

Page 48: ...Printed in Japan LDCL039700 0 S0418 0 1 1 1 Sakada Oizumi machi Ora gun Gunma 370 0596 Japan PHC Corporation 2018...

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