PerkinElmer RamanMicro 300 Series, Getting Started Manual

Page: 74 / 102

74 . RamanMicro 300 Getting Started Guide
13. If higher magnification is required, select the next most powerful objective lens on the
microscope.
NOTE: Do not touch the glass of the objective lenses when you select the appropriate
magnification.
14. On the Hardware tab select the appropriate objective lens from the Beam path drop-
down list.
15. Focus on the sample and again move the sample in x and y directions.
The crosshair indicates the spot where analysis takes place.
16. When the sample is correctly positioned, switch off the illumination source.
17. Select the Dark Field (DF) switch position on the Olympus illuminator module.
You are now ready to acquire data. For more information see SOP-6 and SOP-7.
Remember, there is no “best” objective. High-magnification objective lenses give the most
efficient Raman collection from thin samples, like films or wafers, but low magnification
objectives are better for macro samples such as tablets, bulk plastics and tissue samples.
NOTE: Raman spectra are measured in the visible and near-infrared spectrum. Room lights
and sunlight may contaminate your spectra. Take care not to misinterpret room lights
or sunlight features as Raman features.
SOP-5: Sample Positioning using a Motorized Stage
NOTE: The RamanMicro 300 system can be purchased with either a manual stage or with a
motorized stage.
WARNING
When you are using a motorized stage, do not place your fingers
between the moving and fixed parts of the stage. The motors driving
the stage are powerful and do not stall easily.
1. Follow SOP-1 to turn the instrument on.
2. Load the appropriate sample holder onto the sample stage.