Using the RamanMicro 300 . 73
SOP-4: Sample Positioning using a Manual Stage
NOTE: The RamanMicro 300 system can be purchased with either a manual stage or with a
motorized stage.
1.
Lower the microscope sample stage.
2.
Place the sample on a microscope slide and place it on the stage.
Figure 25 Positioning the sample under the objective lens
3.
Select the 5x objective lens on the microscope.
4.
Move the sample so that it is approximately lined up below the objective lens.
5.
Select the Bright Field (BF) switch position on the Olympus illuminator module.
6.
Switch on the illumination source.
The switch is located on the right side of the microscope frame.
7.
Set the required light intensity using the adjustment knob on the right side of the
microscope frame base.
8.
In Spectrum software, click
to open the Scan and Instrument Setup dialog.
9.
On the Hardware tab select Microscope 5x from the Beam path drop-down list.
10.
Click
to open the Alignment Camera window.
11.
Focus on the sample using the microscope z-axis control knob.
Take care not to crush the sample. The working distances of the objective lenses
supplied as standard are 20 mm (5x), 1.3 mm (20x) and 0.38 mm (50x).
12.
Accurately position the sample in the x and y directions using the stage control knobs.
Place your
sample here
Summary of Contents for RamanMicro 300 Series
Page 1: ...RamanMicro 300 Series Getting Started Guide MOLECULAR SPECTROSCOPY...
Page 5: ...Introduction...
Page 10: ...10 RamanMicro 300 Getting Started Guide...
Page 11: ...Safety Practices...
Page 21: ...Raman History Theory and Instrumentation...
Page 29: ...Preparing Your Facility...
Page 35: ...Installing Your RamanMicro 300...
Page 68: ...68 RamanMicro 300 Getting Started Guide...
Page 69: ...Using the RamanMicro 300...
Page 90: ...90 RamanMicro 300 Getting Started Guide...