Series
16
4-4
Assembling the Phase Contrast Device
Fig.36
4-4-1
Assembling and Operation of Phase
Contrast Flapper
(1)
Keep the phase contrast flapper
①
face up
(upward the face with word), insert it from left to
right into the condenser flapper socket as the
direction of the arrow pointed (see Fig. 36).
(2)
Every
diaphragm
or
hole
has
its
corresponding position, one diaphragm or hole is
inserted into the center of optical path when it
sounds “ka-da” during phase contrast flapper
rotating.
(3)
When observing phase contrast, keep the
adjusting ring of the aperture diaphragm
②
indicator to “PH” position.
★
Each
magnification
phase
contrast
objective
is
used
with
matched
ring
diaphragm (Like 10X phase contrast objective
corresponding to 10X ring diaphragm).
★
As the ring diaphragm is pre-centered, it
doesn’t need to be adjusted in operation in
normal cases.
Fig.37
4-4-2
Assembling and Operation of Disc Phase
Contrast Condenser
(1)
Assembling of Condenser please refer to
2-2-1
,
change the objective on the nosepiece to phase
contrast objective, Center the phase contrast
condenser before use.
(2)
Rotate the phase contrast ring
②
to “BF”
position in bright field observation. When it
sounds “ka-da” in rotating, it indicates that one
diaphragm or hole is rotated into the center of
optical path (see Fig. 37).
4-4-2-1
Centering Halo
In phase contrast microscopy observation, slide
the aperture diaphragm lever
①
to the most left
as the direction of the arrow pointed (always
keep the aperture diaphragm at maximum). (See
Fig. 37)
(1)
Put specimen on the stage and focus.
(2)
Take out one observation eyepiece and insert
one CT (centering telescope) into the tube
(3)
Confirm the corresponding phase ring (in
phase contrast objective) and halo (in phase
contrast disc
②
) are moved into optical path.
