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4.0 USING THE MICROSCOPE
4.1
Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then
lock the lock-screw.
4.2
Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the clamps. Ensure that the specimen is cen
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tred over the stage opening.
4.3
Lamp settings
The microscope is fitted with power white LED illuminator. Before turning on the light, read the section
6.3 about electrical safety precautions. Insert the plug of the power supply into the jack on the rear.
Turn the brightness adjustment knob in order to switch the light on and to reach a brightness suitable
for observation.
4.4
Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary distan
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ce by turning the two parts until one circle of light can be seen.
4.5
Focus and diopter adjustment
Rotate the coarse focusing knob to bring the slide into focus using an objective with a low magnifi
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cation. Adjust the fine focusing knob to get the image sharp and clear while observing with your right
eye, then turn the dioptric adjustment ring on the left eyepiece until a sharp and clear image can be
observed also with the other eye. Turn the tension-adjust-knob to get a suitable tension for the focus
system. When the image is in focus, turn the nosepiece to choose the objective you need.
4.6
Make a polarization analysis
Insert both the swing-out polarizer filter and the analyzer filter (which is placed under the observation
head). Rotate now the polarizer filter until you have a dark background (“extinction condition”). By ro
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tating the stage it’s possible now to observe the polarization effects of your sample. You can lock the
position of the rotating stage through the screw on the back of the stage.
4.7
Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus control
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ling image contrast and resolution.
INSERT THE POLARIZER
FILTER
