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Olympus FV1000                                                                         -3-                                                                  Short instructions 
 

2. General guidelines 

 

1.

 

Lab rules: 

This room is a S2 laboratory.  

At times when S2 experiments are performed in this room, you are not 
allowed to enter the room. S2 experiments will be indicated by a sign at the 
outside of the door and in the online booking system. 

When no S2 experiments are performed everyone who is authorized by the 
facility staff can enter the room.  

You always have to wear a lab coat. Lab coats can be left in the cupboard, 
first door left from the entrance.  

2.

 

Booking and keys: 

Before you can use a microscope you have to make a reservation in the online 
booking system.  

Always pick up a key from Wolfgang Posselt in room 1052. Office hours: Monday 
to Friday. If you are planning to use a microscope outside these hours, pick up a 
key in advance. 

When you are leaving lock the microscope room and after 17:00 also look the 
main door of the facility. Return the key to Wolfgang Posselt or to the mail box of 
the Laser Microscopy Facility, level S, in the hallway left from the stair case, 
second row, in the center.  

3.

 

Climatisation control: 

Please note in the online reservation the desired temperature. 

Wolfgang Posselt will switch on the temperature control early in the morning to 
ensure that the incubation chamber has reached the temperature when you start 
your experiment. 

But you are responsible for setting CO

2

 and humidity as well as for switching off 

the climatisation control. Read chapter 4. “Using the climatic chamber”. 

4.

 

Immersion oil: 

Always remove old immersion oil, before you add new ones. 

Only use the lens tissues provided by the Laser Microscopy Facility staff.  

In case there is oil on no-oil objectives, don’t try to clean them but leave a note 
in the log book. 

Summary of Contents for Fluoview FV1000

Page 1: ...the microscope system For a detailed instruction in system operation refer to the Olympus user manual This instruction also contains important information on laser safety and general guidelines Conten...

Page 2: ...r eyesight may be permanently damaged A reflected laser beam is just as dangerous as a direct beam 3 Never deactivate the laser protection devices Read the Safety Guide in the Olympus User s Manual to...

Page 3: ...hese hours pick up a key in advance When you are leaving lock the microscope room and after 17 00 also look the main door of the facility Return the key to Wolfgang Posselt or to the mail box of the L...

Page 4: ...7 Lasers Lasers for the lines 491 532 and 559 nm have to be switched on manually Read chapter 3 for details 8 Data transfer Never store any data on the microscope computer There are three ways to tra...

Page 5: ...nm are controlled entirely through the Olympus software These lasers are switched on by selecting an acquisition protocol from the dye list in the Image Acquisition window or by directly clicking on...

Page 6: ...able to avoid condensation which will damage the microscope equipment 5 If you are using the CO2 open the gas bottle at the wall behind the microscope table Turn the main valve counter clockwise not m...

Page 7: ...microscope 1 Exit the control software 2 Shut down Windows 3 Turn the key switch of the 491 532 nm laser to off no 4 4 Turn the key switch of the 559 nm laser to off no 5 5 Switch off all buttons of...

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