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Olympus FV1000                                                                         -2-                                                                  Short instructions 
 

1. Laser safety 

 

Safety notes for operating the laser scanning microscope:  

During scanning, the laser radiation is freely accessible after exiting the objective in 
the specimen area of the laser scanning microscope. This circumstance demands 
special attention and caution. If the laser radiation comes in contact with the eyes, it 
may cause serious eye injuries. For this reason, prudent handling is absolutely 
necessary as soon as one or several laser emission warning indicators are lit. If used as 
prescribed and observing the safety notes during the operation of a laser scanning 
microscope, there are no dangers to the user.  

1.

 

According to IEC/EN 60825

1, this laser scanning microscope is a laser 

device of Class 3B. 

2.

 

Never look directly into a laser beam or a reflection of the laser beam. Avoid 
all contact with the laser beam. Otherwise, your eyesight may be permanently 
damaged. A reflected laser beam is just as dangerous as a direct beam. 

3.

 

Never deactivate the laser protection devices. Read the “Safety Guide” in the 
Olympus User’s Manual to familiarize yourself with the safety devices of the 
laser scanning microscope. If you find any of the laser protection devices 
inactivated, please contact the facility staff immediately. 

4.

 

Note that objects (such as micromanipulators) in the specimen area may cause 
laser light to exit the safe beam path during the scanning in an uncontrolled 
manner by means of reflection or scattering and endanger the environment. 

5.

 

Do not change a specimen during scanning.  

6.

 

Do not change any objectives during scanning.  

7.

 

All unused positions of the objective turret should be closed with a cap. 

8.

 

Do not change any filter cubes or beam splitters during scanning. Never 
disconnect an optical waveguide. 

9.

 

Never remove the scan head from the microscope stand during operation. 
Before removing the scan head, the system must be completely switched off.  

10.

 

Do not remove the microscope condenser. 

 

Summary of Contents for Fluoview FV1000

Page 1: ...the microscope system For a detailed instruction in system operation refer to the Olympus user manual This instruction also contains important information on laser safety and general guidelines Conten...

Page 2: ...r eyesight may be permanently damaged A reflected laser beam is just as dangerous as a direct beam 3 Never deactivate the laser protection devices Read the Safety Guide in the Olympus User s Manual to...

Page 3: ...hese hours pick up a key in advance When you are leaving lock the microscope room and after 17 00 also look the main door of the facility Return the key to Wolfgang Posselt or to the mail box of the L...

Page 4: ...7 Lasers Lasers for the lines 491 532 and 559 nm have to be switched on manually Read chapter 3 for details 8 Data transfer Never store any data on the microscope computer There are three ways to tra...

Page 5: ...nm are controlled entirely through the Olympus software These lasers are switched on by selecting an acquisition protocol from the dye list in the Image Acquisition window or by directly clicking on...

Page 6: ...able to avoid condensation which will damage the microscope equipment 5 If you are using the CO2 open the gas bottle at the wall behind the microscope table Turn the main valve counter clockwise not m...

Page 7: ...microscope 1 Exit the control software 2 Shut down Windows 3 Turn the key switch of the 491 532 nm laser to off no 4 4 Turn the key switch of the 559 nm laser to off no 5 5 Switch off all buttons of...

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