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Glossary of Terms Used in the Optical Characteristics Table

Working distance (WD)

: The distance from the top of specimen and the front lens of objective.

Number of aperture (NA) : Important figure determining the objective characteristics (resolution, focal depth and bright-

ness).
Resolution ............. Increases in proportion with the NA.
Focal depth ......... Decreases in proportion with the NA.
Brightness ............. Proportional with the square of NA (comparison under the same magnification).

Resolution

: The limit that an objective can identify the images of two points that are close to each other,

expressed as the distance between the two points on the specimen.

Depth of focus

: The maximum depth of the specimen at which the entire specimen can be brought into focus

simultaneously. This value increases when the aperture iris diaphragm is narrowed and de-
creases when the objective NA is increased.

Field number

: The diameter of the image area that can be observed through the eyepieces, expressed in mm.

Field of view

: The diameter of the area observable on the specimen, expressed in mm.

Summary of Contents for BX-RLA2

Page 1: ...ons under the reflected light when it is installed on the BX51RF BX51TRF system microscope frame together with the BXFM focusing unit To ensure the safety obtain optimum performance and to familiarize...

Page 2: ......

Page 3: ...e sure to read this chapter for safe use of the equipment 1 2 1 SYSTEM DIAGRAM 3 2 NOMENCLATURE OF BX RLA2 4 3 REFLECTED LIGHT BRIGHTFIELD DARKFIELD OBSERVATION PROCEDURE 5 6 4 USING THE CONTROLS OF B...

Page 4: ...stened with commercially available absolute alcohol Since the absolute alcohol is highly flammable it must be handled carefully Be sure to keep it away from open flames or potential sources of electri...

Page 5: ...ruction manual The following symbols are used to set off text in this instruction manual Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and or...

Page 6: ...e for metallurgic specimens or the specimen holder with the stage plate for edsier operation Reflected Light Lamp Housing Module Reflected Light Brightfield Darkfield Illuminator BX RLA2 Stage for Met...

Page 7: ...insertion slot Page 14 Mirror selector lever Page 7 ND filter knob Page 10 FS centering screws Page 7 Field iris diaphragm FS knob Page 7 Filter insertion slot Page 11 Always insert filters from the...

Page 8: ...P 10 Select the light path trinocular tube only Light path selector knob Place the specimen on the stage Stage plate X Y axis knobs Engage the 10X objective in the light path Revolving nosepiece Bring...

Page 9: ...BX RLA2 6 Make a photocopy of the observation procedure page and post it near your microscope Insertion from the left...

Page 10: ...bservations other than DIC be sure to push in the dummy slider in order to prevent flare Fig 2 2 Centering the Field Iris Diaphragm FS Fig 2 1 Slide the mirror selector lever to BF 2 Engage the 10X ob...

Page 11: ...is diaphragm adjusts the illuminated area to obtain an image with high contrast According to the objective in use adjust the FS knob of the reflected light illuminator until the iris image circumscrib...

Page 12: ...two AS centering screws using the Allen screwdriver Using the Aperture Iris Diaphragm In reflected light brightfield observation optimum observation is gener ally possible by setting the aperture to b...

Page 13: ...ghtfield BF Releasing Interlocking The ND filter knob has been interlocked at the factory If brightness is not enough during brightfield DIC or other observation the interlocking can be released 1 Loo...

Page 14: ...ast in monochrome obser vation Used in monochrome photography U 25Y48 Yellow filter Contrast filter for observation of semicon ductor wafers U 25ND25 2 Light intensity adjustment filter Adjusts the br...

Page 15: ...should be parallel and planar Otherwise the reflected light cannot reach the objective rendering observation impossible When observing very large specimens remove the slide plate and place the specim...

Page 16: ...arent specimen and observe it 2 Using the Y Axis Lock Lever Fig 9 This mechanism is provided only with the U SIC4R2 SIC4L2 stage 1 When the Y axis movement lock lever is locked by setting the lever in...

Page 17: ...prevent the polarizer from being burnt Fig 10 1 Setting the Analyzer and Polarizer Fig 10 In the current step do not engage the DIC slider in the light path 1 Bring the specimen in approximate focusin...

Page 18: ...g 12 1 Loosen the mounting knob on the front of the DIC revolving nosepiece insert the DIC slider so that the surface with indication faces up and clamp by tightening the mounting knob 2 With the U DI...

Page 19: ...r the applicable objectives are as follows DIC Slider Applicable Objectives U DICRH MPLFLN MPLFLN BD series UMPlanFl UMPlanFl BD series MPlanFl BD series MPlanApo20X 100X U DICRHC LMPLFLN LMPLFLN BD s...

Page 20: ...the DIC slider as described below Fig 12 2 When the slider movement knob on the DIC slider is turned the interfer ence color in the background varies continuously from the gray sensitive color to mage...

Page 21: ...observation with high contrast thanks to the highest sensibility of the gray sensitive color Selecting a magenta sensitive color allows even small phase variation to be observed as a change in color...

Page 22: ...ervation For the preparation of reflected light simplified polarized light observation perform the operation in 1 Setting the Analyzer and Polarizer in section 6 2 Reflected Light Nomarski DIC Observa...

Page 23: ...stem optical system Reflected light illumination Reflected light illuminator BX RLA2 tube magnification 1X super widefield FN 26 5 compatible Available observations Reflected light brightfield Reflect...

Page 24: ...ocus m Field of view mm MPLN Plan Achromat FN22 MPLN BD Brightfield darkfield Plan Achromat FN22 5X 0 10 20 0 3 36 50X 98 4 4 10X 0 25 10 6 1 34 100X 18 2 2 20X 0 40 1 3 0 0 84 200X 6 1 1 1 50X 0 75 0...

Page 25: ...X 0 6 0 18 Marking MPlanFLN MPlanFLN BD MPLFLN BDP Reflected Polarized Light Plan Semi Apochromat FN26 5 MPlanFLN BDP LMPLFLN Long WD Plan Semi Apochromat FN26 5 LMPlanFLN LMPLFLN BD Brightfield darkf...

Page 26: ...0X 0 95 0 35 0 0 35 1000X 0 67 0 22 1000X 0 67 0 27 100X 0 90 0 31 0 0 37 1000X 0 73 0 22 1000X 0 73 0 27 Marking MPlanApo MPlanApo BD SLMPlan Superlong WD Plan Achromat FN26 5 SLMPlan Optical charact...

Page 27: ...rection with 2 wavelengths red and bleu FL Semi Apochromat or color aberration correction with visual wave lengths bluish purple to red APO Apochromat or color aberration correction with all visual do...

Page 28: ...on under the same magnification Resolution The limit that an objective can identify the images of two points that are close to each other expressed as the distance between the two points on the specim...

Page 29: ...bservation stage is replaced the back side of the stage plate may wear out after long period of use because the top side of biological observation stage is coated with ceramic Rotary wafer holder BH2...

Page 30: ...crew holes on the microscope frame For the present it is not required to align the illuminator s mounting screws with the microscope s mounting screw holes 2 Apply the illuminator toward the rear of t...

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Page 32: ...rg Germany One Corporate Drive Orangeburg NY 10962 U S A 2 8 Honduras Street London EC1Y OTX United Kingdom 31 Gilby Road Mt Waverley VIC 3149 Melbourne Australia 6100 Blue Lagoon Drive Suite 390 Miam...

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